2024
Mutation-induced shift of the photosystem II active site reveals insight into conserved water channels
Flesher D, Liu J, Wang J, Gisriel C, Yang K, Batista V, Debus R, Brudvig G. Mutation-induced shift of the photosystem II active site reveals insight into conserved water channels. Journal Of Biological Chemistry 2024, 300: 107475. PMID: 38879008, PMCID: PMC11294709, DOI: 10.1016/j.jbc.2024.107475.Peer-Reviewed Original ResearchOxygen-evolving complexPhotosystem II active sitePhotosystem IIJahn-Teller distortionPhotosystem II complexD1-Asp170Jahn-TellerResolution cryo-EM structureMutation-induced structural changesCryo-EM structureMagnetic propertiesD1 subunitActive siteOxygenic photosynthesisMutagenesis studiesLight-driven water oxidationSpectroscopic propertiesStructural basisSpectroscopic dataAmino acidsWater oxidation mechanismPhotosystemMutationsMutation-induced shiftWater oxidation
1997
The Tetranuclear Manganese Cluster in Photosystem II: Location and Magnetic Properties of the S2 State As Determined by Saturation−Recovery EPR Spectroscopy †
Koulougliotis D, Schweitzer R, Brudvig G. The Tetranuclear Manganese Cluster in Photosystem II: Location and Magnetic Properties of the S2 State As Determined by Saturation−Recovery EPR Spectroscopy †. Biochemistry 1997, 36: 9735-9746. PMID: 9245405, DOI: 10.1021/bi970326t.Peer-Reviewed Original ResearchConceptsFirst excited spin stateSpin-lattice relaxationSpin statesMagnetic propertiesExcited spin statesS2-state multiline EPRNuclear spin-lattice relaxationSpin-lattice relaxation propertiesEffective magnetic momentLine shapeUntreated photosystem IIMagnetic momentDifferent spin statesDipolar modelNear resonanceGround spin stateSaturation-recovery EPR spectroscopyNuclear relaxation processesLarmor frequencyOrbach mechanismGreater relaxation enhancementTemperature-dependent enhancementDipole-dipole interactionTetranuclear manganese clusterS2-state multiline EPR signal
1995
High-valent oxomanganese clusters: structural and mechanistic work relevant to the oxygen-evolving center in photosystem II
Manchanda R, Brudvig G, Crabtree R. High-valent oxomanganese clusters: structural and mechanistic work relevant to the oxygen-evolving center in photosystem II. Coordination Chemistry Reviews 1995, 144: 1-38. DOI: 10.1016/0010-8545(95)01147-h.Peer-Reviewed Original ResearchO2-evolving centerActive siteOxygen-evolving centerPhotosystem IIOxomanganese clusterBioinorganic chemistryInorganic chemistsWater oxidationComplex chemistryMechanistic aspectsBiophysical studiesChemistryMagnetic propertiesNative enzymeMechanistic workPS IINuclearityChemistsMetalloproteinsSuch clustersConsiderable interestOxidationRecent effortsChemicalsTetramer
1990
Electron Spin-Lattice Relaxation of the Stable Tyrosine Radical D+ in Photosystem II
Beck W, Innes J, Brudvig G. Electron Spin-Lattice Relaxation of the Stable Tyrosine Radical D+ in Photosystem II. 1990, 817-820. DOI: 10.1007/978-94-009-0511-5_188.Peer-Reviewed Original ResearchMn complexesO2-evolving centerDark stable S1 statePhotosystem IIPhotosynthetic H2O oxidationElectron paramagnetic resonance spectraRedox-active sitesWeak dipolar couplingsParamagnetic resonance spectraH2O oxidationOxidation stateTyrosine radicalsDipolar couplingsResonance spectraS0 stateS1 stateTyr-160D2 polypeptidesRelaxation propertiesSpin-lattice relaxation rateMagnetic propertiesElectron spin-lattice relaxation rateSpin-lattice relaxationComplexesRelaxation rate
1987
The tetranuclear manganese complex of Photosystem II
Brudvig G. The tetranuclear manganese complex of Photosystem II. Journal Of Bioenergetics And Biomembranes 1987, 19: 91-104. PMID: 3034873, DOI: 10.1007/bf00762719.Peer-Reviewed Original ResearchConceptsManganese complexesElectron paramagnetic resonance (EPR) spectroscopic studiesManganese ionsS2 stateTetranuclear manganese complexPhotosystem IIFour-electron oxidationIntermediate oxidation statesDisplacement of O2Coordination chemistryOxidation stateO bondSpectroscopic studiesS4 stateMagnetic propertiesComplexesIonsLike structureManganeseMn4O4ChemistryStructureBondsOxidationPropertiesFormation of the S2 state and structure of the Mn complex in photosystem II lacking the extrinsic 33 kilodalton polypeptide
Miller A, de Paula J, Brudvig G. Formation of the S2 state and structure of the Mn complex in photosystem II lacking the extrinsic 33 kilodalton polypeptide. Photosynthesis Research 1987, 12: 205-218. PMID: 24435688, DOI: 10.1007/bf00055121.Peer-Reviewed Original ResearchS2-state multiline EPR signalState multiline EPR signalStable charge separationPSII membranesMultiline EPR signalEPR signalCharge separationManganese sitesElectron paramagnetic resonance spectroscopyUntreated PSII membranesElectron transfer eventsParamagnetic resonance spectroscopyPhotosystem II membranesMn complexesElectron donorResonance spectroscopyS2 stateReaction centersExtrinsic polypeptidesHigh yieldsMagnetic propertiesPhotosystem IISeparationTemperature rangeSpectroscopy