2020
A human secretome library screen reveals a role for Peptidoglycan Recognition Protein 1 in Lyme borreliosis
Gupta A, Arora G, Rosen CE, Kloos Z, Cao Y, Cerny J, Sajid A, Hoornstra D, Golovchenko M, Rudenko N, Munderloh U, Hovius JW, Booth CJ, Jacobs-Wagner C, Palm NW, Ring AM, Fikrig E. A human secretome library screen reveals a role for Peptidoglycan Recognition Protein 1 in Lyme borreliosis. PLOS Pathogens 2020, 16: e1009030. PMID: 33175909, PMCID: PMC7657531, DOI: 10.1371/journal.ppat.1009030.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBorrelia burgdorferiCytokinesGene LibraryHumansLyme DiseaseMiceMice, Inbred BALB CConceptsPeptidoglycan recognition protein 1B. burgdorferi infectionB. burgdorferiBurgdorferi infectionHost responseLow serum IgG levelsCommon vector-borne illnessDiverse host responsesSerum IgG levelsProtein 1Vector-borne illnessImmune dysregulationIgG levelsBorreliacidal activityBurgdorferi sensu latoHuman extracellular proteinsSpirochete burdenTick biteB. burgdorferi sensu latoSpirochete Borrelia burgdorferiNervous systemLyme borreliosisLyme diseaseInfectionBorrelia burgdorferi
2003
Borrelia burgdorferi transcriptome in the central nervous system of non-human primates
Narasimhan S, Camaino M, Liang FT, Santiago F, Laskowski M, Philipp MT, Pachner AR, Radolf JD, Fikrig E. Borrelia burgdorferi transcriptome in the central nervous system of non-human primates. Proceedings Of The National Academy Of Sciences Of The United States Of America 2003, 100: 15953-15958. PMID: 14671329, PMCID: PMC307674, DOI: 10.1073/pnas.2432412100.Peer-Reviewed Original ResearchConceptsNon-human primate modelB. burgdorferiEffect of dexamethasoneCentral nervous systemHost metabolic pathwaysNon-human primatesNeurological symptomsPaucibacillary natureCNS milieuImmune statusCommon manifestationPrimate modelNervous systemExpression profilesLyme diseaseHost factorsHeart tissueGene expressionB. burgdorferi transcriptomeBorrelia burgdorferiNHPNeuroborreliosisImmunocompetentVivo gene expressionBurgdorferi
2002
A novel family of anticoagulants from the saliva of Ixodes scapularis
Narasimhan S, Koski RA, Beaulieu B, Anderson JF, Ramamoorthi N, Kantor F, Cappello M, Fikrig E. A novel family of anticoagulants from the saliva of Ixodes scapularis. Insect Molecular Biology 2002, 11: 641-650. PMID: 12421422, DOI: 10.1046/j.1365-2583.2002.00375.x.Peer-Reviewed Original ResearchConceptsN-terminal amino acid sequenceSalivary gland cDNA libraryAmino acid sequenceN-terminal sequenceCDNA libraryAcid sequenceAnticoagulant proteinsIxodes scapularisMolecular approachesTerminal sequenceIntrinsic pathwayEscherichia coliSpecific inhibitorNovel familyProteinSalp14Different functionsTick salivaSequenceBiological activityDose-dependent mannerProtease inhibitorsReversed-phase HPLCParaloguesScapularis
2001
Salp25D, an Ixodes scapularis Antioxidant, Is 1 of 14 Immunodominant Antigens in Engorged Tick Salivary Glands
Das S, Banerjee G, DePonte K, Marcantonio N, Kantor F, Fikrig E. Salp25D, an Ixodes scapularis Antioxidant, Is 1 of 14 Immunodominant Antigens in Engorged Tick Salivary Glands. The Journal Of Infectious Diseases 2001, 184: 1056-1064. PMID: 11574922, DOI: 10.1086/323351.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsAntigensAntioxidantsCattleCloning, MolecularConsensus SequenceEnzyme-Linked Immunosorbent AssayFemaleGene LibraryGlutathione PeroxidaseGuinea PigsHumansImmunoglobulin GInsect ProteinsIxodesMiceMice, Inbred C3HMolecular Sequence DataNematodaRabbitsRNA, MessengerSalivary GlandsSequence Homology, Amino AcidSpecies SpecificityTime Factors
2000
SALP16, a gene induced in Ixodes scapularis salivary glands during tick feeding.
Das S, Marcantonio N, Deponte K, Telford SR, Anderson JF, Kantor FS, Fikrig E. SALP16, a gene induced in Ixodes scapularis salivary glands during tick feeding. American Journal Of Tropical Medicine And Hygiene 2000, 62: 99-105. PMID: 10761732, DOI: 10.4269/ajtmh.2000.62.99.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsBase SequenceBlotting, WesternEnzyme-Linked Immunosorbent AssayFeeding BehaviorFemaleGene Expression RegulationGene LibraryGuinea PigsIxodesMiceMice, Inbred C3HMolecular Sequence DataPolymerase Chain ReactionRandom Amplified Polymorphic DNA TechniqueRecombinant ProteinsRNASalivary GlandsSalivary Proteins and PeptidesSequence Analysis, DNATick Infestations