2021
A human apolipoprotein L with detergent-like activity kills intracellular pathogens
Gaudet RG, Zhu S, Halder A, Kim BH, Bradfield CJ, Huang S, Xu D, Mamiñska A, Nguyen TN, Lazarou M, Karatekin E, Gupta K, MacMicking JD. A human apolipoprotein L with detergent-like activity kills intracellular pathogens. Science 2021, 373 PMID: 34437126, PMCID: PMC8422858, DOI: 10.1126/science.abf8113.Peer-Reviewed Original ResearchMeSH KeywordsApolipoproteins LBacterial Outer MembraneBacteriolysisCell MembraneCell Membrane PermeabilityCells, CulturedCRISPR-Cas SystemsCytosolDetergentsGene EditingGram-Negative BacteriaGTP-Binding ProteinsHumansImmunity, InnateInterferon-gammaLipoproteinsMicrobial ViabilityO AntigensProtein DomainsSalmonella typhimuriumSolubilityConceptsSingle-particle cryo-electron microscopyCell-autonomous defenseCytosol-invasive bacteriaExpression of hundredsNative mass spectrometryCryo-electron microscopyHuman genesDetergent-like activityHost proteinsLipoprotein nanodiscsMammalian lipidsExtracellular transportImmune cytokine interferonCell typesDetergent-like propertiesApolipoprotein LLife-threatening infectionsPotent bactericidal agentsAnionic membranesProteinCytokine interferonNonimmune cellsMass spectrometryCellsMutagenesis
2000
Neuromelanin biosynthesis is driven by excess cytosolic catecholamines not accumulated by synaptic vesicles
Sulzer D, Bogulavsky J, Larsen K, Behr G, Karatekin E, Kleinman M, Turro N, Krantz D, Edwards R, Greene L, Zecca L. Neuromelanin biosynthesis is driven by excess cytosolic catecholamines not accumulated by synaptic vesicles. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 11869-11874. PMID: 11050221, PMCID: PMC17261, DOI: 10.1073/pnas.97.22.11869.Peer-Reviewed Original ResearchConceptsCytosolic catecholaminesSubstantia nigraDisease neurodegenerationCytosolic dopamineRat substantia nigraSynaptic vesiclesParkinson's disease neurodegenerationHuman substantia nigraIron chelator desferrioxamineCatecholamine neuronsFunction of neuromelaninDeep brain regionsParkinson's diseaseAdenoviral-mediated overexpressionBrain regionsDouble-membrane autophagic vacuolesVesicular accumulationPC12 cell culturesChelator desferrioxamineDopamineNM granulesAntioxidant mechanismsPC12 cellsNeuromelaninNeurite outgrowth