2018
Toward a unified picture of the exocytotic fusion pore
Karatekin E. Toward a unified picture of the exocytotic fusion pore. FEBS Letters 2018, 592: 3563-3585. PMID: 30317539, PMCID: PMC6353554, DOI: 10.1002/1873-3468.13270.Peer-Reviewed Original Research
2016
Nanodisc-cell fusion: control of fusion pore nucleation and lifetimes by SNARE protein transmembrane domains
Wu Z, Auclair SM, Bello O, Vennekate W, Dudzinski NR, Krishnakumar SS, Karatekin E. Nanodisc-cell fusion: control of fusion pore nucleation and lifetimes by SNARE protein transmembrane domains. Scientific Reports 2016, 6: 27287. PMID: 27264104, PMCID: PMC4893671, DOI: 10.1038/srep27287.Peer-Reviewed Original ResearchConceptsFusion poreTransmembrane domainPore dynamicsProtein transmembrane domainNeurotransmitter-filled vesiclesT-SNAREsPlasma membraneRecycling kineticsPore lifetimePore currentsFlickering poresPore stabilityMultiple timesZipperingNanodiscsDomainProteinVesiclesMembraneCellsAssaysCognatesPore propertiesPoresCholesterol Increases the Openness of SNARE-Mediated Flickering Fusion Pores
Stratton BS, Warner JM, Wu Z, Nikolaus J, Wei G, Wagnon E, Baddeley D, Karatekin E, O’Shaughnessy B. Cholesterol Increases the Openness of SNARE-Mediated Flickering Fusion Pores. Biophysical Journal 2016, 110: 1538-1550. PMID: 27074679, PMCID: PMC4833774, DOI: 10.1016/j.bpj.2016.02.019.Peer-Reviewed Original ResearchConceptsT-SNAREsFusion poreSoluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteinsLipid bilayer propertiesFactor attachment protein receptor proteinsN-ethylmaleimide-sensitive factor attachment protein receptor proteinsTotal internal reflection fluorescence microscopyCognate t-SNAREsFusion pore dynamicsPhysiological cholesterol levelsProtein receptor proteinsFlickering fusion poresReflection fluorescence microscopyIndividual fusion eventsV-SNAREBilayer propertiesFusion eventsPore flickeringReceptor proteinFluorescence microscopySmall unilamellar vesiclesLipid compositionFusion siteSnareExocytotic release