2004
Paracellular Cl- permeability is regulated by WNK4 kinase: Insight into normal physiology and hypertension
Kahle KT, MacGregor GG, Wilson FH, Van Hoek AN, Brown D, Ardito T, Kashgarian M, Giebisch G, Hebert SC, Boulpaep EL, Lifton RP. Paracellular Cl- permeability is regulated by WNK4 kinase: Insight into normal physiology and hypertension. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 14877-14882. PMID: 15465913, PMCID: PMC522037, DOI: 10.1073/pnas.0406172101.Peer-Reviewed Original ResearchConceptsPseudohypoaldosteronism type IIPHAII-mutant WNK4Paracellular fluxPotent antihypertensive agentTight junction proteinsTight junctionsAntihypertensive agentsParacellular ion fluxPharmacologic propertiesTight junction structureTranscellular transportersWild-type WNK4Normal physiologyHypertensionTransepithelial resistanceWNK signalingKidney epitheliumTight junction formationParacellular pathwayWNK4Effect of WNK4EpitheliumType IIWNK4 kinaseHomeostasis
2003
Active K+ secretion through multiple KCa-type channels and regulation by IKCa channels in rat proximal colon
Joiner WJ, Basavappa S, Vidyasagar S, Nehrke K, Krishnan S, Binder HJ, Boulpaep EL, Rajendran VM. Active K+ secretion through multiple KCa-type channels and regulation by IKCa channels in rat proximal colon. AJP Gastrointestinal And Liver Physiology 2003, 285: g185-g196. PMID: 12606302, DOI: 10.1152/ajpgi.00337.2002.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsApaminCarbacholChloridesCHO CellsCholinergic AgonistsClotrimazoleColonCricetinaeGene ExpressionGrowth InhibitorsIntermediate-Conductance Calcium-Activated Potassium ChannelsMaleMembrane PotentialsMolecular Sequence DataPatch-Clamp TechniquesPeptidesPotassiumPotassium ChannelsPotassium Channels, Calcium-ActivatedPotassium, DietaryRatsRats, Sprague-DawleyRubidium RadioisotopesConceptsChannel inhibitors clotrimazoleRat proximal colonProximal colonSK channel inhibitorsAddition of thapsigarginExpression of Ca2Muscarinic receptorsCholinergic agentsMuscarinic agonistsEpithelial stripsIKCa channelsMucosal additionSerosal surfaceColonic epitheliumIK channelsIntracellular Ca2Channel inhibitorsRSK4 mRNAColonWestern blottingIK proteinSecretionColonic cryptsPrecise mechanismConfocal immunofluorescence
2000
An electroneutral sodium/bicarbonate cotransporter NBCn1 and associated sodium channel
Choi I, Aalkjaer C, Boulpaep E, Boron W. An electroneutral sodium/bicarbonate cotransporter NBCn1 and associated sodium channel. Nature 2000, 405: 571-575. PMID: 10850716, DOI: 10.1038/35014615.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsBicarbonatesBiological TransportCarrier ProteinsChloridesCloning, OrganismDNA, ComplementaryElectrophysiologyHumansMolecular Sequence DataRatsRecombinant ProteinsReverse Transcriptase Polymerase Chain ReactionRNA, ComplementarySequence Homology, Amino AcidSodiumSodium ChannelsSodium-Bicarbonate SymportersXenopus
1998
Transcellular Chloride Pathways in Ambystoma Proximal Tubule
Abdulnour-Nakhoul S, Boulpaep E. Transcellular Chloride Pathways in Ambystoma Proximal Tubule. The Journal Of Membrane Biology 1998, 166: 15-35. PMID: 9784583, DOI: 10.1007/s002329900444.Peer-Reviewed Original ResearchMeSH KeywordsAmbystomaAnimalsCell MembraneChloride ChannelsChloridesIon TransportMembrane PotentialsSodiumConceptsProximal tubulesChloride channel blockerContribution of transportersAmbystoma proximal tubuleTransepithelial potential differenceDisulfonic stilbeneCl-HCO3 exchangeFree RingerLines of evidenceTubulesBasolateral cell membraneLumenNa-independent ClMembrane potentialBasolateral membraneApical membrane potentialAiClBlockers
1993
Whole-cell currents in single and confluent M-1 mouse cortical collecting duct cells.
Korbmacher C, Segal A, Fejes-Tóth G, Giebisch G, Boulpaep E. Whole-cell currents in single and confluent M-1 mouse cortical collecting duct cells. The Journal Of General Physiology 1993, 102: 761-793. PMID: 8270912, PMCID: PMC2229168, DOI: 10.1085/jgp.102.4.761.Peer-Reviewed Original ResearchConceptsWhole-cell currentsWhole-cell conductanceConfluent cellsWhole-cell membrane currentsBasolateral K channelsCells 24 hK conductancePA/cellMicroM glibenclamideAmiloride-sensitive conductanceTransgenic miceReabsorb sodiumOutward currentsM amilorideGlibenclamide inhibitionDuct cellsPrincipal cellsMembrane currentsBath KLarge depolarizationChloride conductanceK channelsLipophilic drugsPermeable supportsMice
1990
Bicarbonate transport mechanisms in the Ambystoma kidney proximal tubule: transepithelial potential measurements.
Bock J, Boulpaep E. Bicarbonate transport mechanisms in the Ambystoma kidney proximal tubule: transepithelial potential measurements. The Yale Journal Of Biology And Medicine 1990, 63: 529-47. PMID: 2092412, PMCID: PMC2589409.Peer-Reviewed Original Research
1983
Chloride transport across the basolateral cell membrane of theNecturus proximal tubule: Dependence on bicarbonate and sodium
Guggino W, London R, Boulpaep E, Giebisch G. Chloride transport across the basolateral cell membrane of theNecturus proximal tubule: Dependence on bicarbonate and sodium. The Journal Of Membrane Biology 1983, 71: 227-240. PMID: 6302263, DOI: 10.1007/bf01875464.Peer-Reviewed Original ResearchConceptsBasolateral cell membraneProximal tubule cellsBasolateral solutionNH4Cl exposurePH-sensitive microelectrodesProximal tubulesTubule cellsPerfusion solutionCell membraneIntracellular chlorideTheNecturus proximal tubuleIntracellular pHApical cell membraneNH4Cl treatmentChloride transportChloride activityKidneyIntracellular pH regulation in the renal proximal tubule of the salamander. Basolateral HCO3- transport.
Boron W, Boulpaep E. Intracellular pH regulation in the renal proximal tubule of the salamander. Basolateral HCO3- transport. The Journal Of General Physiology 1983, 81: 53-94. PMID: 6833997, PMCID: PMC2215562, DOI: 10.1085/jgp.81.1.53.Peer-Reviewed Original ResearchConceptsProximal tubulesRenal proximal tubulesRapid fallBasolateral HCO3- transportBasolateral membraneTiger salamander Ambystoma tigrinumReversible fallNominal absencePresence of HCO3Removal of ClTransient depolarizationSimilar reductionBasolateral membrane potentialSalamander Ambystoma tigrinumHCO3- transportHCO3- transportersMembrane potentialOnly minor effectsAforementioned changesTubulesCl-sensitive microelectrodesDepolarizationConstant PCO2Opposite effectAmbystoma tigrinumIntracellular pH regulation in the renal proximal tubule of the salamander. Na-H exchange.
Boron W, Boulpaep E. Intracellular pH regulation in the renal proximal tubule of the salamander. Na-H exchange. The Journal Of General Physiology 1983, 81: 29-52. PMID: 6833996, PMCID: PMC2215563, DOI: 10.1085/jgp.81.1.29.Peer-Reviewed Original ResearchConceptsProximal tubulesProximal tubule cellsRenal proximal tubulesTiger salamander Ambystoma tigrinumPH-sensitive microelectrodesFree RingerMaximal effectTubule cellsTransient riseDiuretic amilorideBasolateral sideBasolateral solutionExponential time courseSalamander Ambystoma tigrinumTime courseCl removalMaximal rateBasolateral membraneNormal rateTubulesAmbystoma tigrinumPH regulationCellsActive transportRecovery
1982
Electrical properties of chloride transport across theNecturus proximal tubule
Guggino W, Boulpaep E, Giebisch G. Electrical properties of chloride transport across theNecturus proximal tubule. The Journal Of Membrane Biology 1982, 65: 185-196. PMID: 7062339, DOI: 10.1007/bf01869962.Peer-Reviewed Original Research
1981
Isolated perfused salamander proximal tubule. II. Monovalent ion replacement and rheogenic transport
Sackin H, Boulpaep E. Isolated perfused salamander proximal tubule. II. Monovalent ion replacement and rheogenic transport. American Journal Of Physiology 1981, 241: f540-f555. PMID: 7304748, DOI: 10.1152/ajprenal.1981.241.5.f540.Peer-Reviewed Original Research
1978
Factors influencing transepithelial potential difference in mammalian distal tubule
Hayslett J, Boulpaep E, Giebisch G. Factors influencing transepithelial potential difference in mammalian distal tubule. American Journal Of Physiology 1978, 234: f182-f191. PMID: 629352, DOI: 10.1152/ajprenal.1978.234.3.f182.Peer-Reviewed Original ResearchSegmental heterogeneity and effects of organic solutes on ion transport across the Nectums proximal tubule studied with electrophysiological techniques
Steels P, Boulpaep E. Segmental heterogeneity and effects of organic solutes on ion transport across the Nectums proximal tubule studied with electrophysiological techniques. Archives Of Physiology And Biochemistry 1978, 86: 688-689. PMID: 83844, DOI: 10.3109/13813457809055945.Peer-Reviewed Original Research
1975
Models for coupling of salt and water transport; Proximal tubular reabsorption in Necturus kidney.
Sackin H, Boulpaep E. Models for coupling of salt and water transport; Proximal tubular reabsorption in Necturus kidney. The Journal Of General Physiology 1975, 66: 671-733. PMID: 1104761, PMCID: PMC2226230, DOI: 10.1085/jgp.66.6.671.Peer-Reviewed Original ResearchConceptsCoupling of saltWater transportDifferent boundary conditionsChemical driving forceSolvent fluxWater fluxTransport parametersBoundary conditionsGradient modelConcentration profilesOsmotic driving forceDriving forceIsotonic transportNet transportDiamondFluxTransportForceWaterFirst modelSecond modelModel