2010
Substrate specificity of Rhbg: ammonium and methyl ammonium transport
Nakhoul N, Abdulnour-Nakhoul S, Boulpaep E, Rabon E, Schmidt E, Hamm L. Substrate specificity of Rhbg: ammonium and methyl ammonium transport. American Journal Of Physiology - Cell Physiology 2010, 299: c695-c705. PMID: 20592240, PMCID: PMC2944323, DOI: 10.1152/ajpcell.00019.2010.Peer-Reviewed Original ResearchMouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct
Lu M, Dong K, Egan ME, Giebisch GH, Boulpaep EL, Hebert SC. Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 6082-6087. PMID: 20231442, PMCID: PMC2851921, DOI: 10.1073/pnas.0902661107.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzoatesChloride ChannelsCyclic AMPCyclic AMP-Dependent Protein KinasesCystic Fibrosis Transmembrane Conductance RegulatorFemaleIn Vitro TechniquesKidney CortexKidney Tubules, CollectingKineticsMiceMice, Inbred C57BLMice, Inbred CFTRMice, KnockoutMice, TransgenicMutationOocytesPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingRecombinant ProteinsThiazolidinesXenopus laevisConceptsCystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorCl- channel activityConductance regulatorCl- channelsApical membrane proteinsExpression of CFTRChannel activityCFTR Cl- channelApical chloride channelApical cell membraneDeltaF508 CFTR mutationMembrane proteinsCatalytic subunitXenopus laevis oocytesForm proteinPrincipal cellsCFTR channelsROMK null miceApical patchesApical membraneSingle-channel conductanceChloride channelsCell membrane
2006
CFTR is required for PKA-regulated ATP sensitivity of Kir1.1 potassium channels in mouse kidney
Lu M, Leng Q, Egan ME, Caplan MJ, Boulpaep EL, Giebisch GH, Hebert SC. CFTR is required for PKA-regulated ATP sensitivity of Kir1.1 potassium channels in mouse kidney. Journal Of Clinical Investigation 2006, 116: 797-807. PMID: 16470247, PMCID: PMC1361349, DOI: 10.1172/jci26961.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCurcuminCyclic AMP-Dependent Protein KinasesCystic Fibrosis Transmembrane Conductance RegulatorHydrogen-Ion ConcentrationKidneyMiceMice, Inbred C57BLMice, Inbred CFTRMice, TransgenicMutationOocytesPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingXenopus laevisConceptsFunctional switchCystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channelATP sensitivityEffects of CFTRThick ascending limbPotential physiological rolePKA activityRenal K channelsCystic fibrosisPhysiological roleSecretory channelsK channelsRenal tubule epithelial cellsApical membraneCFTRDeltaF508 mutationDistal nephron segmentsCl- channelsK homeostasisTubule epithelial cellsEpithelial cellsTAL cellsPotassium channelsK handlingGlibenclamide sensitivity
2005
Requirement of Voltage-Gated Calcium Channel ß4 Subunit for T Lymphocyte Functions
Badou A, Basavappa S, Desai R, Peng YQ, Matza D, Mehal WZ, Kaczmarek LK, Boulpaep EL, Flavell RA. Requirement of Voltage-Gated Calcium Channel ß4 Subunit for T Lymphocyte Functions. Science 2005, 307: 117-121. PMID: 15637280, DOI: 10.1126/science.1100582.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium Channels, L-TypeCalcium SignalingCD4-Positive T-LymphocytesCytokinesDNA-Binding ProteinsIon Channel GatingLymphocyte ActivationMembrane PotentialsMiceMice, Inbred C3HMice, Inbred C57BLMutationNFATC Transcription FactorsNuclear ProteinsPatch-Clamp TechniquesPhosphorylationProtein SubunitsReceptors, Antigen, T-CellT-LymphocytesTranscription FactorsConceptsT lymphocytesCalcium channelsVoltage-gated calcium channelsT lymphocyte functionT cell receptor stimulationCell receptor stimulationCytokine productionLymphocyte functionCalcium influxReceptor stimulationCalcium responseCalcium entryTranscription factor NFATCav1 channelsLymphocytesAlpha1 subunitCav channelsNormal functionNonexcitable cellsDisplay impairmentsExcitable cellsChannel openingMolecular identityDiverse physiological processesPhysiological processes
2004
Paracellular Cl- permeability is regulated by WNK4 kinase: Insight into normal physiology and hypertension
Kahle KT, MacGregor GG, Wilson FH, Van Hoek AN, Brown D, Ardito T, Kashgarian M, Giebisch G, Hebert SC, Boulpaep EL, Lifton RP. Paracellular Cl- permeability is regulated by WNK4 kinase: Insight into normal physiology and hypertension. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 14877-14882. PMID: 15465913, PMCID: PMC522037, DOI: 10.1073/pnas.0406172101.Peer-Reviewed Original ResearchConceptsPseudohypoaldosteronism type IIPHAII-mutant WNK4Paracellular fluxPotent antihypertensive agentTight junction proteinsTight junctionsAntihypertensive agentsParacellular ion fluxPharmacologic propertiesTight junction structureTranscellular transportersWild-type WNK4Normal physiologyHypertensionTransepithelial resistanceWNK signalingKidney epitheliumTight junction formationParacellular pathwayWNK4Effect of WNK4EpitheliumType IIWNK4 kinaseHomeostasis
2003
Calcium-dependent, swelling-activated K+ conductance in human neuroblastoma cells
Basavappa S, Mangel A, Boulpaep E. Calcium-dependent, swelling-activated K+ conductance in human neuroblastoma cells. Biochemical And Biophysical Research Communications 2003, 308: 759-763. PMID: 12927783, DOI: 10.1016/s0006-291x(03)01481-5.Peer-Reviewed Original ResearchConceptsHuman neuroblastoma cell line CHP-100CHP-100Regulatory volume decreaseCell-attached patch-clamp studiesFree extracellular solutionHuman neuroblastoma cellsSwelling-activated ClPatch-clamp studiesFura-2Extracellular osmolarityFree extracellularNeuroblastoma cellsMOsm/Hypoosmotic solutionCell swellingExtracellular solutionCl- channelsHypoosmotic stressPresent studyMost mammalian cellsEGTACellsVolume decreaseEffluxPrevious studiesActive K+ secretion through multiple KCa-type channels and regulation by IKCa channels in rat proximal colon
Joiner WJ, Basavappa S, Vidyasagar S, Nehrke K, Krishnan S, Binder HJ, Boulpaep EL, Rajendran VM. Active K+ secretion through multiple KCa-type channels and regulation by IKCa channels in rat proximal colon. AJP Gastrointestinal And Liver Physiology 2003, 285: g185-g196. PMID: 12606302, DOI: 10.1152/ajpgi.00337.2002.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsApaminCarbacholChloridesCHO CellsCholinergic AgonistsClotrimazoleColonCricetinaeGene ExpressionGrowth InhibitorsIntermediate-Conductance Calcium-Activated Potassium ChannelsMaleMembrane PotentialsMolecular Sequence DataPatch-Clamp TechniquesPeptidesPotassiumPotassium ChannelsPotassium Channels, Calcium-ActivatedPotassium, DietaryRatsRats, Sprague-DawleyRubidium RadioisotopesConceptsChannel inhibitors clotrimazoleRat proximal colonProximal colonSK channel inhibitorsAddition of thapsigarginExpression of Ca2Muscarinic receptorsCholinergic agentsMuscarinic agonistsEpithelial stripsIKCa channelsMucosal additionSerosal surfaceColonic epitheliumIK channelsIntracellular Ca2Channel inhibitorsRSK4 mRNAColonWestern blottingIK proteinSecretionColonic cryptsPrecise mechanismConfocal immunofluorescence
1998
Properties of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule
Mauerer U, Boulpaep E, Segal A. Properties of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule. The Journal Of General Physiology 1998, 111: 139-160. PMID: 9417141, PMCID: PMC1887768, DOI: 10.1085/jgp.111.1.139.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmbystomaAnimalsBariumBiological TransportCationsCells, CulturedCytidine TriphosphateDiazoxideDiureticsElectric ConductivityGlyburideGuanosine TriphosphateHypoglycemic AgentsInosine TriphosphateIon Channel GatingKidney Tubules, ProximalKineticsMicrovilliPatch-Clamp TechniquesPotassium ChannelsSodium Chloride Symporter InhibitorsSodium-Potassium-Exchanging ATPaseThalliumThymine NucleotidesUridine TriphosphateConceptsProximal tubule cellsProximal tubulesTubule cellsBasolateral membraneChannel opener diazoxideChannel activityPump-leak couplingRenal proximal tubulesPivotal physiological rolesOpener diazoxideMillimolar dosesMM ATPHigh dosesPotassium conductanceCell membrane potentialInternal Mg2Cytosolic additionATPase pumpRegulation of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule
Mauerer U, Boulpaep E, Segal A. Regulation of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule. The Journal Of General Physiology 1998, 111: 161-180. PMID: 9417142, PMCID: PMC1887764, DOI: 10.1085/jgp.111.1.161.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmbystomaAnimalsBiological TransportCalciumCell MembraneColforsinCyclic AMP-Dependent Protein KinasesEnzyme ActivationEpithelial CellsHydrogen-Ion ConcentrationIon Channel GatingKidney Tubules, ProximalPatch-Clamp TechniquesPhosphorylationPotassium ChannelsProtein Kinase CSodium-Potassium-Exchanging ATPaseConceptsBasolateral membraneProtein kinase AActin cytoskeletonATPase pumpOuter membraneProtein kinaseKinase ACellular metabolismKATP channelsSecond messengerStimulation of transportTransport activityPhysiological importanceMembrane stretchPhorbol esterChannel activityMembrane patchesRegulationIntracellularO patchesFunctional couplingHypotonic swellingCell-attached patchesCell depolarizationRenal proximal tubules
1997
Tubule electrophysiology: from single channels back to the renal epithelium.
Boulpaep E. Tubule electrophysiology: from single channels back to the renal epithelium. Wiener Klinische Wochenschrift 1997, 109: 489-92. PMID: 9261991.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneHumansIon ChannelsKidney TubulesMembrane PotentialsPatch-Clamp TechniquesWater-Electrolyte BalanceConceptsMacroscopic membrane propertiesMicroscopic parametersSingle channel proteinsElectrical propertiesIon flowMacroscopic referenceSingle barrierMembrane patchesRenal epitheliumSingle channelRenal ion transportRenal ion channelsRegulatory domainExperimental errorChannel proteinsPore conductivityIon channelsWhole-cell conductancePatch-clamp technologyBasolateral membraneRenal tubule cellsCell membrane parametersIon selectivityIon transportOpen probability
1995
A Calcium-activated and nucleotide-sensitive nonselective cation channel in M-1 mouse cortical collecting duct cells
Korbmacher C, Volk T, Segal A, Boulpaep E, Frömter E. A Calcium-activated and nucleotide-sensitive nonselective cation channel in M-1 mouse cortical collecting duct cells. The Journal Of Membrane Biology 1995, 146: 29-45. PMID: 7563035, DOI: 10.1007/bf00232678.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCell LineCell MembraneIon ChannelsKidney CortexKidney Tubules, CollectingMiceNucleotidesPatch-Clamp TechniquesConceptsSensitive nonselective cation channelNSC channelNonselective cation channelsDuct cellsAmiloride-sensitive sodium absorptionCation channelsChannel activityAdenine nucleotidesVoltage-dependent blockCalcium entryNonselective cation channel activitySodium absorptionCation channel activityCytoplasmic calciumInhibitory effectSpecial physiological conditionsCytoplasmic applicationMiceCGMP-dependent protein kinaseFlufenamic acidSingle-channel current recordingsMajor routeVolume regulationCurrent recordingsCGMP