1998
Transcellular Chloride Pathways in Ambystoma Proximal Tubule
Abdulnour-Nakhoul S, Boulpaep E. Transcellular Chloride Pathways in Ambystoma Proximal Tubule. The Journal Of Membrane Biology 1998, 166: 15-35. PMID: 9784583, DOI: 10.1007/s002329900444.Peer-Reviewed Original ResearchConceptsProximal tubulesChloride channel blockerContribution of transportersAmbystoma proximal tubuleTransepithelial potential differenceDisulfonic stilbeneCl-HCO3 exchangeFree RingerLines of evidenceTubulesBasolateral cell membraneLumenNa-independent ClMembrane potentialBasolateral membraneApical membrane potentialAiClBlockers
1997
The electrogenic Na/HCO3 cotransporter.
Boron W, Fong P, Hediger M, Boulpaep E, Romero M. The electrogenic Na/HCO3 cotransporter. Wiener Klinische Wochenschrift 1997, 109: 445-56. PMID: 9261985.Peer-Reviewed Original ResearchConceptsElectrogenic Na/HCO3 cotransporterProximal tubulesHCO3- cotransporterRenal proximal tubulesCO2/10 mM HCO3HCO3- transportersRegulation of intracellularBasolateral membraneNumber of tissuesNative oocytesRapid hyperpolarizationXenopus laevis oocytesExtracellular ClBicarbonate cotransporterOocyte expressionCotransporterMM HCO3OocytesCell typesMost cellsMembrane potentialAmino acid homologySingle cloneWater-injected controlsTubules
1987
Electrochemical analysis of renal Na+-glucose cotransport in salamander proximal tubules
Morgunov N, Boulpaep E. Electrochemical analysis of renal Na+-glucose cotransport in salamander proximal tubules. American Journal Of Physiology 1987, 252: f154-f169. PMID: 3812699, DOI: 10.1152/ajprenal.1987.252.1.f154.Peer-Reviewed Original ResearchConceptsLuminal glucoseProximal tubulesFavorable chemical gradientFavorable electrical gradientSodium concentrationSodium-glucose cotransporterIntracellular sodium activityElectrogenic responseSodium activityMM D-glucoseMembrane potentialSalamander proximal tubuleLuminal perfusateOmega X cm2Glucose cotransportGlucose concentrationLuminal membranePhysiological rangeX cm2Luminal membrane potentialGlucoseD-glucoseTubulesLuminal membrane resistancePerfusate
1986
Regulation of single potassium ion channels from apical membrane of rabbit collecting tubule
Hunter M, Lopes A, Boulpaep E, Giebisch G. Regulation of single potassium ion channels from apical membrane of rabbit collecting tubule. American Journal Of Physiology 1986, 251: f725-f733. PMID: 2429562, DOI: 10.1152/ajprenal.1986.251.4.f725.Peer-Reviewed Original ResearchConceptsCortical collecting tubuleCollecting tubuleRabbit cortical collecting tubuleBath calcium concentrationOpen probabilityPatch-clamp techniqueOne-half maximalApical membranePotassium secretionChannel open timeIntracellular calciumPotassium ion channelsChannel open probabilityCalcium concentrationMM calciumChannel activityTubulesIon channelsBath solutionSecretionOpen timeTransmembrane potentialCalciumFunction of calciumApparent Ki
1984
Single channel recordings of calcium-activated potassium channels in the apical membrane of rabbit cortical collecting tubules.
Hunter M, Lopes A, Boulpaep E, Giebisch G. Single channel recordings of calcium-activated potassium channels in the apical membrane of rabbit cortical collecting tubules. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 4237-4239. PMID: 6330754, PMCID: PMC345404, DOI: 10.1073/pnas.81.13.4237.Peer-Reviewed Original ResearchConceptsPotassium channelsRabbit cortical collecting tubuleCalcium concentrationCalcium-activated potassium channelsDose-dependent fashionCortical collecting tubulePatch-clamp techniqueCollecting tubuleSingle potassium channelsApical membraneOpen channel probabilitySingle-channel recordingsSlope conductanceChannel activityTubulesChannel recordingsRecordings
1983
Rheogenic transport in the renal proximal tubule.
Sackin H, Boulpaep E. Rheogenic transport in the renal proximal tubule. The Journal Of General Physiology 1983, 82: 819-851. PMID: 6319539, PMCID: PMC2228722, DOI: 10.1085/jgp.82.6.819.Peer-Reviewed Original ResearchIntracellular pH regulation in the renal proximal tubule of the salamander. Basolateral HCO3- transport.
Boron W, Boulpaep E. Intracellular pH regulation in the renal proximal tubule of the salamander. Basolateral HCO3- transport. The Journal Of General Physiology 1983, 81: 53-94. PMID: 6833997, PMCID: PMC2215562, DOI: 10.1085/jgp.81.1.53.Peer-Reviewed Original ResearchConceptsProximal tubulesRenal proximal tubulesRapid fallBasolateral HCO3- transportBasolateral membraneTiger salamander Ambystoma tigrinumReversible fallNominal absencePresence of HCO3Removal of ClTransient depolarizationSimilar reductionBasolateral membrane potentialSalamander Ambystoma tigrinumHCO3- transportHCO3- transportersMembrane potentialOnly minor effectsAforementioned changesTubulesCl-sensitive microelectrodesDepolarizationConstant PCO2Opposite effectAmbystoma tigrinumIntracellular pH regulation in the renal proximal tubule of the salamander. Na-H exchange.
Boron W, Boulpaep E. Intracellular pH regulation in the renal proximal tubule of the salamander. Na-H exchange. The Journal Of General Physiology 1983, 81: 29-52. PMID: 6833996, PMCID: PMC2215563, DOI: 10.1085/jgp.81.1.29.Peer-Reviewed Original ResearchConceptsProximal tubulesProximal tubule cellsRenal proximal tubulesTiger salamander Ambystoma tigrinumPH-sensitive microelectrodesFree RingerMaximal effectTubule cellsTransient riseDiuretic amilorideBasolateral sideBasolateral solutionExponential time courseSalamander Ambystoma tigrinumTime courseCl removalMaximal rateBasolateral membraneNormal rateTubulesAmbystoma tigrinumPH regulationCellsActive transportRecovery
1979
Effect of amiloride on the apical cell membrane cation channels of a sodium-absorbing, potassium-secreting renal epithelium
O'Neil R, Boulpaep E. Effect of amiloride on the apical cell membrane cation channels of a sodium-absorbing, potassium-secreting renal epithelium. The Journal Of Membrane Biology 1979, 50: 365-387. PMID: 513119, DOI: 10.1007/bf01868898.Peer-Reviewed Original ResearchConceptsLumen-negative transepithelial potential differenceActive Na absorptionApical cell membraneEffects of amilorideEquivalent short-circuit currentTransepithelial potential differenceNa absorptionMaximum inhibitory concentrationAmilorideDiuretic amilorideCation channelsCell membraneNa channelsInhibitory concentrationRenal epitheliumMembrane cation channelsRemoval of NaAbsence of NaTight junctionsPerfusateK permeabilityTubulesLumen
1978
Factors influencing transepithelial potential difference in mammalian distal tubule
Hayslett J, Boulpaep E, Giebisch G. Factors influencing transepithelial potential difference in mammalian distal tubule. American Journal Of Physiology 1978, 234: f182-f191. PMID: 629352, DOI: 10.1152/ajprenal.1978.234.3.f182.Peer-Reviewed Original ResearchElectrophysiological Measurements on the Renal Tubule
Boulpaep E, Giebisch G. Electrophysiological Measurements on the Renal Tubule. 1978, 165-193. DOI: 10.1007/978-1-4615-8894-8_7.Peer-Reviewed Original Research
1977
Electrical characteristics of the mammalian distal tubule: Comparison of Ling-Gerard and macroelectrodes
Hayslett J, Boulpaep E, Kashgarian M, Giebisch G. Electrical characteristics of the mammalian distal tubule: Comparison of Ling-Gerard and macroelectrodes. Kidney International 1977, 12: 324-331. PMID: 604621, DOI: 10.1038/ki.1977.119.Peer-Reviewed Original Research