1997
Expression cloning and characterization of a renal electrogenic Na+ /HCO3− cotransporter
Romero M, Hediger M, Boulpaep E, Boron W. Expression cloning and characterization of a renal electrogenic Na+ /HCO3− cotransporter. Nature 1997, 387: 409-413. PMID: 9163427, DOI: 10.1038/387409a0.Peer-Reviewed Original ResearchConceptsPotential membrane-spanning domainsMembrane-spanning domainsElectrogenic sodium bicarbonate cotransporterBinding of inhibitorsSodium bicarbonate cotransporterAnimal cellsBicarbonate transportersElectrogenic transportersSuccessful cloningExpression cloningComplementary DNAPrincipal regulatorAmino acidsBicarbonate cotransporterProtein purificationFunctional familiesXenopus oocytesMolecular informationTransportersCloningReproductive systemSalamander Ambystoma tigrinumProteinAmbystoma tigrinumIntracellular pH
1984
Quantitative ultrastructure and functional correlates in proximal tubule of Ambystoma and Necturus
Maunsbach A, Boulpaep E. Quantitative ultrastructure and functional correlates in proximal tubule of Ambystoma and Necturus. American Journal Of Physiology 1984, 246: f710-f724. PMID: 6720973, DOI: 10.1152/ajprenal.1984.246.5.f710.Peer-Reviewed Original ResearchConceptsBasal cell membraneCell membraneStructure-function correlationsIntercellular spacesBasal cell surfaceLateral cell membranesBasal extracellular labyrinthMorphometric comparative analysisVolume of cellsInterspecies variabilityCell surfaceAmbystomaQuantitative ultrastructureElaborate organizationBasolateral membraneLateral intercellular spacesSpeciesSalamander Ambystoma tigrinumExtracellular channelsMembraneUltrastructureAmbystoma tigrinumFunctional propertiesProximal tubules
1983
Intracellular pH regulation in the renal proximal tubule of the salamander. Basolateral HCO3- transport.
Boron W, Boulpaep E. Intracellular pH regulation in the renal proximal tubule of the salamander. Basolateral HCO3- transport. The Journal Of General Physiology 1983, 81: 53-94. PMID: 6833997, PMCID: PMC2215562, DOI: 10.1085/jgp.81.1.53.Peer-Reviewed Original ResearchConceptsProximal tubulesRenal proximal tubulesRapid fallBasolateral HCO3- transportBasolateral membraneTiger salamander Ambystoma tigrinumReversible fallNominal absencePresence of HCO3Removal of ClTransient depolarizationSimilar reductionBasolateral membrane potentialSalamander Ambystoma tigrinumHCO3- transportHCO3- transportersMembrane potentialOnly minor effectsAforementioned changesTubulesCl-sensitive microelectrodesDepolarizationConstant PCO2Opposite effectAmbystoma tigrinumIntracellular pH regulation in the renal proximal tubule of the salamander. Na-H exchange.
Boron W, Boulpaep E. Intracellular pH regulation in the renal proximal tubule of the salamander. Na-H exchange. The Journal Of General Physiology 1983, 81: 29-52. PMID: 6833996, PMCID: PMC2215563, DOI: 10.1085/jgp.81.1.29.Peer-Reviewed Original ResearchConceptsProximal tubulesProximal tubule cellsRenal proximal tubulesTiger salamander Ambystoma tigrinumPH-sensitive microelectrodesFree RingerMaximal effectTubule cellsTransient riseDiuretic amilorideBasolateral sideBasolateral solutionExponential time courseSalamander Ambystoma tigrinumTime courseCl removalMaximal rateBasolateral membraneNormal rateTubulesAmbystoma tigrinumPH regulationCellsActive transportRecovery