2010
Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct
Lu M, Dong K, Egan ME, Giebisch GH, Boulpaep EL, Hebert SC. Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 6082-6087. PMID: 20231442, PMCID: PMC2851921, DOI: 10.1073/pnas.0902661107.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzoatesChloride ChannelsCyclic AMPCyclic AMP-Dependent Protein KinasesCystic Fibrosis Transmembrane Conductance RegulatorFemaleIn Vitro TechniquesKidney CortexKidney Tubules, CollectingKineticsMiceMice, Inbred C57BLMice, Inbred CFTRMice, KnockoutMice, TransgenicMutationOocytesPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingRecombinant ProteinsThiazolidinesXenopus laevisConceptsCystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorCl- channel activityConductance regulatorCl- channelsApical membrane proteinsExpression of CFTRChannel activityCFTR Cl- channelApical chloride channelApical cell membraneDeltaF508 CFTR mutationMembrane proteinsCatalytic subunitXenopus laevis oocytesForm proteinPrincipal cellsCFTR channelsROMK null miceApical patchesApical membraneSingle-channel conductanceChloride channelsCell membrane
1984
Quantitative ultrastructure and functional correlates in proximal tubule of Ambystoma and Necturus
Maunsbach A, Boulpaep E. Quantitative ultrastructure and functional correlates in proximal tubule of Ambystoma and Necturus. American Journal Of Physiology 1984, 246: f710-f724. PMID: 6720973, DOI: 10.1152/ajprenal.1984.246.5.f710.Peer-Reviewed Original ResearchConceptsBasal cell membraneCell membraneStructure-function correlationsIntercellular spacesBasal cell surfaceLateral cell membranesBasal extracellular labyrinthMorphometric comparative analysisVolume of cellsInterspecies variabilityCell surfaceAmbystomaQuantitative ultrastructureElaborate organizationBasolateral membraneLateral intercellular spacesSpeciesSalamander Ambystoma tigrinumExtracellular channelsMembraneUltrastructureAmbystoma tigrinumFunctional propertiesProximal tubules
1983
Rheogenic transport in the renal proximal tubule.
Sackin H, Boulpaep E. Rheogenic transport in the renal proximal tubule. The Journal Of General Physiology 1983, 82: 819-851. PMID: 6319539, PMCID: PMC2228722, DOI: 10.1085/jgp.82.6.819.Peer-Reviewed Original ResearchChloride transport across the basolateral cell membrane of theNecturus proximal tubule: Dependence on bicarbonate and sodium
Guggino W, London R, Boulpaep E, Giebisch G. Chloride transport across the basolateral cell membrane of theNecturus proximal tubule: Dependence on bicarbonate and sodium. The Journal Of Membrane Biology 1983, 71: 227-240. PMID: 6302263, DOI: 10.1007/bf01875464.Peer-Reviewed Original ResearchConceptsBasolateral cell membraneProximal tubule cellsBasolateral solutionNH4Cl exposurePH-sensitive microelectrodesProximal tubulesTubule cellsPerfusion solutionCell membraneIntracellular chlorideTheNecturus proximal tubuleIntracellular pHApical cell membraneNH4Cl treatmentChloride transportChloride activityKidney
1979
Effect of amiloride on the apical cell membrane cation channels of a sodium-absorbing, potassium-secreting renal epithelium
O'Neil R, Boulpaep E. Effect of amiloride on the apical cell membrane cation channels of a sodium-absorbing, potassium-secreting renal epithelium. The Journal Of Membrane Biology 1979, 50: 365-387. PMID: 513119, DOI: 10.1007/bf01868898.Peer-Reviewed Original ResearchConceptsLumen-negative transepithelial potential differenceActive Na absorptionApical cell membraneEffects of amilorideEquivalent short-circuit currentTransepithelial potential differenceNa absorptionMaximum inhibitory concentrationAmilorideDiuretic amilorideCation channelsCell membraneNa channelsInhibitory concentrationRenal epitheliumMembrane cation channelsRemoval of NaAbsence of NaTight junctionsPerfusateK permeabilityTubulesLumen
1978
Factors influencing transepithelial potential difference in mammalian distal tubule
Hayslett J, Boulpaep E, Giebisch G. Factors influencing transepithelial potential difference in mammalian distal tubule. American Journal Of Physiology 1978, 234: f182-f191. PMID: 629352, DOI: 10.1152/ajprenal.1978.234.3.f182.Peer-Reviewed Original Research