1981
Partial purification of RNase P from Schizosaccharomyces pombe.
Kline L, Nishikawa S, Söll D. Partial purification of RNase P from Schizosaccharomyces pombe. Journal Of Biological Chemistry 1981, 256: 5058-5063. PMID: 6262315, DOI: 10.1016/s0021-9258(19)69366-x.Peer-Reviewed Original ResearchConceptsSimple assay procedureNucleic acid componentsColumn chromatographyReaction productsEnzyme purificationPhosphocellulose column chromatographyAcid componentsSynthetic substratesAssay procedurePurificationAcid solubilityPartial purificationSubstrateYeast RNase PSolubilityChromatographyProductsSeparationPhosphateTrichloroacetic acid solubility
1979
The nucleotide sequence of a cloned Drosophila arginine tRNA gene and its in vitro transcription in Xenopus germinal vesicle extracts.
Silverman S, Schmidt O, Söll D, Hovemann B. The nucleotide sequence of a cloned Drosophila arginine tRNA gene and its in vitro transcription in Xenopus germinal vesicle extracts. Journal Of Biological Chemistry 1979, 254: 10290-10294. PMID: 114522, DOI: 10.1016/s0021-9258(19)86707-8.Peer-Reviewed Original ResearchConceptsMature tRNAPrecursor RNANucleotide sequenceDrosophila tRNAArg geneGerminal vesicle extractsArginine tRNA geneXenopus germinal vesicleTRNA genesTRNAArg genePrimary transcriptDNA sequencesLeader sequenceVesicle extractsT residuesGenesTranscriptionGerminal vesicleTerminusVitro systemTRNAA sequenceRNASequenceNucleotidesTRNAArgThe nucleotide sequence of the major glutamate transfer RNA from Schizosaccharomyces pombe
Wong T, McCutchan T, Kohli J, Söll D. The nucleotide sequence of the major glutamate transfer RNA from Schizosaccharomyces pombe. Nucleic Acids Research 1979, 6: 2057-2068. PMID: 379816, PMCID: PMC327836, DOI: 10.1093/nar/6.6.2057.Peer-Reviewed Original ResearchThe nucleotide sequence of a UGA suppressor serine tRNA from Schizosaccharomyces pombe
Rafalski A, Kohli J, Agris P, Söll D. The nucleotide sequence of a UGA suppressor serine tRNA from Schizosaccharomyces pombe. Nucleic Acids Research 1979, 6: 2683-2695. PMID: 461200, PMCID: PMC327885, DOI: 10.1093/nar/6.8.2683.Peer-Reviewed Original Research
1977
Isolation of Escherichia coli precursor tRNAs containing modified nucleoside Q.
Vögeli G, Stewart T, McCutchan T, Söll D. Isolation of Escherichia coli precursor tRNAs containing modified nucleoside Q. Journal Of Biological Chemistry 1977, 252: 2311-2318. PMID: 321455, DOI: 10.1016/s0021-9258(17)40556-4.Peer-Reviewed Original ResearchA rapid method for preparation of calf spleen exonuclease *
Silverman S, Sōll D. A rapid method for preparation of calf spleen exonuclease *. Nucleic Acids Research 1977, 4: 3511-3517. PMID: 200894, PMCID: PMC342668, DOI: 10.1093/nar/4.10.3511.Peer-Reviewed Original Research
1975
A method for the isolation of specific tRNA precursors.
Vögeli G, Grosjean H, Söll D. A method for the isolation of specific tRNA precursors. Proceedings Of The National Academy Of Sciences Of The United States Of America 1975, 72: 4790-4794. PMID: 1108001, PMCID: PMC388817, DOI: 10.1073/pnas.72.12.4790.Peer-Reviewed Original Research
1974
New aspects in tRNA biosynthesis
Schäfer K, Söll D. New aspects in tRNA biosynthesis. Biochimie 1974, 56: 795-804. PMID: 4614860, DOI: 10.1016/s0300-9084(74)80500-6.Peer-Reviewed Original ResearchCovalent attachment of fluorescent groups to transfer ribonucleic acid. Reactions with 4-bromomethyl-7-methoxy-2-oxo-2H-benzopyran.
Yang C, Soell D. Covalent attachment of fluorescent groups to transfer ribonucleic acid. Reactions with 4-bromomethyl-7-methoxy-2-oxo-2H-benzopyran. Biochemistry 1974, 13: 3615-21. PMID: 4367729, DOI: 10.1021/bi00714a033.Peer-Reviewed Original ResearchAlkaline PhosphataseAmino Acyl-tRNA SynthetasesBenzopyransBromineCarbon RadioisotopesEscherichia coliFluorescenceFormatesGlutamatesMethionineMethyl EthersModels, ChemicalNucleosidesPhosphoric Diester HydrolasesPseudouridineRibonucleasesRNA, BacterialRNA, TransferSpectrometry, FluorescenceStructure-Activity RelationshipTransfer RNA AminoacylationUridineN‐(purin‐6‐ylcarbamoyl)threonine: Biosynthesis in vitro in transfer RNA by an enzyme purified from Escherichia coli
Körner A, Söll D. N‐(purin‐6‐ylcarbamoyl)threonine: Biosynthesis in vitro in transfer RNA by an enzyme purified from Escherichia coli. FEBS Letters 1974, 39: 301-306. PMID: 4604806, DOI: 10.1016/0014-5793(74)80135-3.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAmino Acyl-tRNA SynthetasesCarbamatesCarbon RadioisotopesChromatography, DEAE-CelluloseChromatography, Ion ExchangeChromatography, PaperChromatography, Thin LayerElectrophoresis, PaperEscherichia coliIsotope LabelingMutationNitrosoguanidinesPhosphorus RadioisotopesPurine NucleosidesRibonucleasesRNA, BacterialRNA, TransferSpectrophotometry, UltravioletThreonineTime FactorsTritiumFingerprinting nonradioactive ribonucleic acid with the aid of polynucleotide phosphorylase
Szeto K, Söll D. Fingerprinting nonradioactive ribonucleic acid with the aid of polynucleotide phosphorylase. Nucleic Acids Research 1974, 1: 171-182. PMID: 10793669, PMCID: PMC343333, DOI: 10.1093/nar/1.1.171.Peer-Reviewed Original Research
1973
Nucleotide Modification In Vitro of the Precursor of Transfer RNATyr of Escherichia coli
Schaefer K, Altman S, Söll D. Nucleotide Modification In Vitro of the Precursor of Transfer RNATyr of Escherichia coli. Proceedings Of The National Academy Of Sciences Of The United States Of America 1973, 70: 3626-3630. PMID: 4587257, PMCID: PMC427294, DOI: 10.1073/pnas.70.12.3626.Peer-Reviewed Original ResearchThe effect of growth temperatures on the in vivo ribose methylation of Bacillus stearothermophilus transfer RNA
Agris P, Koh H, Söll D. The effect of growth temperatures on the in vivo ribose methylation of Bacillus stearothermophilus transfer RNA. Archives Of Biochemistry And Biophysics 1973, 154: 277-282. PMID: 4689778, DOI: 10.1016/0003-9861(73)90058-1.Peer-Reviewed Original Research
1971
Enzymatic Modification of Transfer RNA
Söll D. Enzymatic Modification of Transfer RNA. Science 1971, 173: 293-299. PMID: 4934576, DOI: 10.1126/science.173.3994.293.Peer-Reviewed Original ResearchConceptsTRNA-modifying enzymesTRNA moleculesTRNA genesTransfer RNAAmino acid biosynthesisParticular tRNA speciesNucleic acid-protein interactionsAminoacyl-tRNA synthetaseSimple selection procedureAmino acid acceptorPrecursor tRNAsActive tRNAMature tRNACellular processesTRNA speciesRegulatory mutantsAcid biosynthesisRNA precursorsProtein factorsIsoacceptor tRNAsDifferent amino acidsNucleotide sequencePolynucleotide levelBiological functionsTRNAThe nucleotide sequence of two leucine tRNA species from Escherichia coli K12
Blank H, Sőll D. The nucleotide sequence of two leucine tRNA species from Escherichia coli K12. Biochemical And Biophysical Research Communications 1971, 43: 1192-1197. PMID: 4936129, DOI: 10.1016/0006-291x(71)90589-4.Peer-Reviewed Original Research