2024
Chronic lung inflammation disrupts the quiescent state of hematopoietic stem cells in a cystic fibrosis mouse model
Braga C, Mancuso R, Thompson E, Oez H, Gudneppanavar R, Zhang P, Huang P, Murray T, Egan M, Krause D, Bruscia E. Chronic lung inflammation disrupts the quiescent state of hematopoietic stem cells in a cystic fibrosis mouse model. The Journal Of Immunology 2024, 212: 0062_6002-0062_6002. DOI: 10.4049/jimmunol.212.supp.0062.6002.Peer-Reviewed Original ResearchHematopoietic stem cellsChronic lung inflammationLung inflammationCystic fibrosisBone marrowQuiescent state of HSCsProgression of CF lung diseaseResponse to airway infectionWT hematopoietic stem cellsExpansion of HSCsMultipotent progenitorsCystic fibrosis mouse modelStem cellsCF lung diseasePathways associated with proliferationNeutrophilic lung inflammationPro-inflammatory signatureFibrosis mouse modelATAC-sequencing analysisAirway infectionBM cellsMyeloid lineageLung diseaseMouse modelInflammation
2019
Adult bone marrow progenitors become decidual cells and contribute to embryo implantation and pregnancy
Tal R, Shaikh S, Pallavi P, Tal A, López-Giráldez F, Lyu F, Fang YY, Chinchanikar S, Liu Y, Kliman HJ, Alderman M, Pluchino N, Kayani J, Mamillapalli R, Krause DS, Taylor HS. Adult bone marrow progenitors become decidual cells and contribute to embryo implantation and pregnancy. PLOS Biology 2019, 17: e3000421. PMID: 31513564, PMCID: PMC6742226, DOI: 10.1371/journal.pbio.3000421.Peer-Reviewed Original ResearchConceptsBM transplantsDecidual cellsPregnancy lossMesenchymal stem cellsAdult bone marrow progenitorsDecidualization-related genesBone marrow progenitorsAdult bone marrowWT donorsPhysiologic contributionSuccessful pregnancyBMDC recruitmentStromal expansionImmune cellsEndometrial cellsDeficient miceUterine expressionUterine tissueDecidual stromaPregnancyBone marrowNonhematopoietic cellsBMDCsHemochorial placentaMarrow progenitors
2013
Very Small Embryonic‐Like Stem Cells from the Murine Bone Marrow Differentiate into Epithelial Cells of the Lung
Kassmer SH, Jin H, Zhang PX, Bruscia EM, Heydari K, Lee JH, Kim CF, Kassmer SH, Krause DS. Very Small Embryonic‐Like Stem Cells from the Murine Bone Marrow Differentiate into Epithelial Cells of the Lung. Stem Cells 2013, 31: 2759-2766. PMID: 23681901, PMCID: PMC4536826, DOI: 10.1002/stem.1413.Peer-Reviewed Original ResearchConceptsEpithelial cellsSmall embryonic-like stem cellsLung epithelial cellsEmbryonic-like stem cellsStem/progenitor cellsStem cellsDonor miceHematopoietic stem/progenitor cellsBM cellsAdult BMBone marrowSmall embryonicNonhematopoietic cellsProgenitor cellsBroad differentiation potentialVSELsEngraftmentLungHigh rateNumerous reportsAdult stem cellsDifferentiation potentialCellsFirst reportReport
2012
Nonhematopoietic Cells are the Primary Source of Bone Marrow‐Derived Lung Epithelial Cells
Kassmer SH, Bruscia EM, Zhang P, Krause DS. Nonhematopoietic Cells are the Primary Source of Bone Marrow‐Derived Lung Epithelial Cells. Stem Cells 2012, 30: 491-499. PMID: 22162244, PMCID: PMC3725285, DOI: 10.1002/stem.1003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsBone Marrow CellsBone Marrow TransplantationCell SeparationEpithelial CellsGene ExpressionLuminescent ProteinsLungMiceMice, 129 StrainMice, Inbred C57BLMice, KnockoutMicroscopy, ConfocalPulmonary Surfactant-Associated Protein CRecombinant ProteinsSingle-Cell AnalysisConceptsLung epithelial cellsNonhematopoietic cellsBM cellsEpithelial cellsBone marrowLungs of miceType 2 pneumocytesNonhematopoietic stem cellsNonhematopoietic fractionAdult BMPrimitive stem cell populationNull miceProgenitor cellsMiceStem cell populationCell populationsMarrowStem cellsMultiple tissuesHematopoietic stemBMCellsPrevious studiesEngraftmentLung
2011
Targeted Gene Modification of Hematopoietic Progenitor Cells in Mice Following Systemic Administration of a PNA-peptide Conjugate
Rogers FA, Lin SS, Hegan DC, Krause DS, Glazer PM. Targeted Gene Modification of Hematopoietic Progenitor Cells in Mice Following Systemic Administration of a PNA-peptide Conjugate. Molecular Therapy 2011, 20: 109-118. PMID: 21829173, PMCID: PMC3255600, DOI: 10.1038/mt.2011.163.Peer-Reviewed Original ResearchConceptsGene modificationGene therapyHematopoietic stem cell gene therapyStem cell gene therapyGenomic modificationsVivo gene therapyCell gene therapyTargeted gene modificationVivo gene modificationHematopoietic progenitor cellsPeptide nucleic acidSystemic administrationBone marrowGene-targeting strategiesProgenitor cellsPrimary recipient miceStem cell mobilizationEx vivo manipulationSickle cell anemiaLymphoid cell lineagesDonor miceRecipient miceHematologic disordersInvasive alternativeCell mobilizationTissue‐engineered vascular grafts form neovessels that arise from regeneration of the adjacent blood vessel
Hibino N, Villalona G, Pietris N, Duncan DR, Schoffner A, Roh JD, Yi T, Dobrucki LW, Mejias D, Sawh‐Martinez R, Harrington JK, Sinusas A, Krause DS, Kyriakides T, Saltzman WM, Pober JS, Shin'oka T, Breuer CK. Tissue‐engineered vascular grafts form neovessels that arise from regeneration of the adjacent blood vessel. The FASEB Journal 2011, 25: 2731-2739. PMID: 21566209, PMCID: PMC3136337, DOI: 10.1096/fj.11-182246.Peer-Reviewed Original ResearchConceptsBone marrow-derived mononuclear cellsSmooth muscle cellsAutologous bone marrow-derived mononuclear cellsMarrow-derived mononuclear cellsMuscle cellsAnalogous mouse modelsAdjacent blood vesselsHuman bone marrow-derived mononuclear cellsMononuclear cellsClinical trialsMouse recipientsImmunodeficient miceComposite graftMouse modelBone marrowMacrophage invasionCell originChimeric hostGraftBlood vesselsHost cell originHost macrophagesNeovessel formationVessel wallNeovessels
2008
Hepatocyte Nuclear Factor‐1 as Marker of Epithelial Phenotype Reveals Marrow‐Derived Hepatocytes, but Not Duct Cells, After Liver Injury in Mice
Swenson ES, Guest I, Ilic Z, Mazzeo‐Helgevold M, Lizardi P, Hardiman C, Sell S, Krause DS. Hepatocyte Nuclear Factor‐1 as Marker of Epithelial Phenotype Reveals Marrow‐Derived Hepatocytes, but Not Duct Cells, After Liver Injury in Mice. Stem Cells 2008, 26: 1768-1777. PMID: 18467658, PMCID: PMC2846397, DOI: 10.1634/stemcells.2008-0148.Peer-Reviewed Original ResearchConceptsMarrow-derived epithelial cellsHepatocyte nuclear factor 1Y chromosomeNuclear factor 1Ductal progenitor cellsLiver injuryInflammatory cellsFemale miceProgenitor cellsEpithelial cellsFactor 1Male bone marrowStable hematopoietic engraftmentBone marrow originColocalization of GFPNuclear markersBone marrow cellsDuctal progenitorsHematopoietic engraftmentChromosomesBone marrowMarrow originPancytokeratin stainingCholangiocyte phenotypeMarrow cellsBone Marrow–derived Cells and Stem Cells in Lung Repair
Krause DS. Bone Marrow–derived Cells and Stem Cells in Lung Repair. Annals Of The American Thoracic Society 2008, 5: 323-327. PMID: 18403327, PMCID: PMC2645242, DOI: 10.1513/pats.200712-169dr.Peer-Reviewed Original ResearchConceptsMarrow-derived epithelial cellsEpithelial cellsBM cellsTissue injuryBone marrow-derived cellsBM-derived cellsMarrow-derived cellsPotential clinical utilityBronchiolar epithelial cellsType II pneumocytesLung damageTracheal epithelial cellsLung repairClinical utilityGI tractBone marrowTissue damagePeer-reviewed studiesNonhematopoietic cell typesBeneficial effectsPotential mechanismsTissue repairLungInjuryTissue microenvironment
2007
Bone Marrow Contributes to Epithelial Cancers in Mice and Humans as Developmental Mimicry
Cogle CR, Theise ND, Fu D, Ucar D, Lee S, Guthrie SM, Lonergan J, Rybka W, Krause DS, Scott EW. Bone Marrow Contributes to Epithelial Cancers in Mice and Humans as Developmental Mimicry. Stem Cells 2007, 25: 1881-1887. PMID: 17478582, DOI: 10.1634/stemcells.2007-0163.Peer-Reviewed Original ResearchConceptsEpithelial cancersEpithelial neoplasiaHematopoietic stem cellsNeoplastic environmentStem cellsHematopoietic cell transplantationBone marrow cellsHuman marrowMarrow involvementMarrow cellsSmall bowelCell transplantationLung neoplasiaMouse modelBone marrowMimicryDistant organsNeoplasiaCancerMarrowStable fusionCellsPhenotypeInductionBowel
2006
Engraftment of Marrow-derived Epithelial Cells
Herzog EL, Krause DS. Engraftment of Marrow-derived Epithelial Cells. Annals Of The American Thoracic Society 2006, 3: 691-695. PMID: 17065375, PMCID: PMC2647654, DOI: 10.1513/pats.200605-109sf.BooksEngraftment of Donor‐Derived Epithelial Cells in Multiple Organs Following Bone Marrow Transplantation into Newborn Mice
Bruscia EM, Ziegler EC, Price JE, Weiner S, Egan ME, Krause DS. Engraftment of Donor‐Derived Epithelial Cells in Multiple Organs Following Bone Marrow Transplantation into Newborn Mice. Stem Cells 2006, 24: 2299-2308. PMID: 16794262, DOI: 10.1634/stemcells.2006-0166.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBone Marrow TransplantationCystic Fibrosis Transmembrane Conductance RegulatorEpithelial CellsFemaleFluorescent Antibody TechniqueHematopoietic Stem Cell TransplantationIn Situ Hybridization, FluorescenceMaleMiceMice, Inbred C57BLMice, Inbred StrainsMice, TransgenicRNA, MessengerY ChromosomeConceptsBone marrow-derived cellsMarrow-derived epithelial cellsBone marrow transplantationNewborn miceEpithelial cellsMarrow transplantationGI tractBone marrow-derived epithelial cellsDonor-derived epithelial cellsDoses of busulfanMarrow-derived cellsEngraftment of donorIrradiated adult recipientsMyeloablative regimenPreparative regimenAdult recipientsDifferent regimensEngrafted miceHematopoietic engraftmentGastrointestinal tractSurvival advantageTherapeutic benefitAdult miceMultiple organsBone marrowAssessment of cystic fibrosis transmembrane conductance regulator (CFTR) activity in CFTR-null mice after bone marrow transplantation
Bruscia EM, Price JE, Cheng EC, Weiner S, Caputo C, Ferreira EC, Egan ME, Krause DS. Assessment of cystic fibrosis transmembrane conductance regulator (CFTR) activity in CFTR-null mice after bone marrow transplantation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 2965-2970. PMID: 16481627, PMCID: PMC1413802, DOI: 10.1073/pnas.0510758103.Peer-Reviewed Original ResearchConceptsCftr-/- miceEpithelial cellsNasal epitheliumBM-derived cellsBone marrow transplantationWild-type BMAirway epithelial cellsCystic fibrosis transmembrane conductance regulator (CFTR) activityCystic fibrosis miceRare epithelial cellsCftr-null miceMarrow transplantationBM transplantationFibrosis miceRespiratory tractCFTR activityGI tractBone marrowGastrointestinalChloride secretionCFTR-dependent chloride secretionIndividual miceTransplantationDifferent dosesMice
2005
Bone marrow plasticity revisited: protection or differentiation in the kidney tubule?
Krause D, Cantley LG. Bone marrow plasticity revisited: protection or differentiation in the kidney tubule? Journal Of Clinical Investigation 2005, 115: 1705-1708. PMID: 16007248, PMCID: PMC1159151, DOI: 10.1172/jci25540.Peer-Reviewed Original ResearchConceptsBone marrow-derived cellsMarrow-derived cellsTubule repairBone marrowEpithelial cellsStem cellsIschemic injuryRenal interstitiumTubular cellsRenal epithelial cellsCell lossTubular segmentsRenal cellsKidney tubulesMajority of dataEndogenous cellsHigh rateRecent reportsKidneyMarrowRepairCellsTubulesProliferationStem cell nicheEngraftment of Bone Marrow‐Derived Epithelial Cells
Krause DS. Engraftment of Bone Marrow‐Derived Epithelial Cells. Annals Of The New York Academy Of Sciences 2005, 1044: 117-124. PMID: 15958704, DOI: 10.1196/annals.1349.015.Peer-Reviewed Original ResearchConceptsBone marrow-derived cellsMarrow-derived cellsEpithelial cellsMarrow-derived epithelial cellsNonhematopoietic cellsLevel of engraftmentCell plasticityStem cell plasticityAllogeneic settingAdult stem cell plasticityBM cellsBuccal mucosaGastrointestinal tractBone marrowTissue damageEngraftmentFunctional epithelial cellsTherapeutic relevanceNonhematopoietic cell typesPrecursor cellsMost reportsDiseased organsLungMarrowDifferent phenotypesEngraftment of bone marrow-derived epithelial cells
Van Arnam JS, Herzog E, Grove J, Bruscia E, Ziegler E, Swenson S, Krause DS. Engraftment of bone marrow-derived epithelial cells. Stem Cell Reviews And Reports 2005, 1: 21-27. PMID: 17132871, DOI: 10.1385/scr:1:1:021.Peer-Reviewed Original ResearchConceptsBM-derived cellsEpithelial cellsBM transplantationBone marrow-derived epithelial cellsMarrow-derived epithelial cellsPreclinical mouse modelsType II pneumocytesHematopoietic systemGene therapyFalse-positive cellsGastrointestinal tractHost epithelial cellsMouse modelFemale recipientsPositive cellsBone marrowCre-lox systemTherapeutic potentialTherapeutic useTissue-specific markersTransplantationTherapyPotential gene therapy applicationsCellsHuman diseases
2004
Bone Marrow-Derived Cells Contribute to Epithelial Engraftment during Wound Healing
Borue X, Lee S, Grove J, Herzog EL, Harris R, Diflo T, Glusac E, Hyman K, Theise ND, Krause DS. Bone Marrow-Derived Cells Contribute to Epithelial Engraftment during Wound Healing. American Journal Of Pathology 2004, 165: 1767-1772. PMID: 15509544, PMCID: PMC1618655, DOI: 10.1016/s0002-9440(10)63431-1.Peer-Reviewed Original ResearchConceptsBone marrow-derived cellsEpithelial cellsMarrow-derived epithelial cellsEngraftment of BMDCsDonor-derived cellsMarrow-derived cellsWound healingDegree of engraftmentLevel of engraftmentAbsence of injuryEarly wound healingFemale miceBone marrowCytokeratin 5Cre-lox systemEngraftmentSkin damageWound edgeKeratinocytesInjuryWound siteTransit-amplifying cellsStem cellsEpidermal stem cellsRecent findingsLack of a Fusion Requirement for Development of Bone Marrow-Derived Epithelia
Harris RG, Herzog EL, Bruscia EM, Grove JE, Van Arnam JS, Krause DS. Lack of a Fusion Requirement for Development of Bone Marrow-Derived Epithelia. Science 2004, 305: 90-93. PMID: 15232107, DOI: 10.1126/science.1098925.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta-GalactosidaseBone Marrow CellsBone Marrow TransplantationCell DifferentiationCell FusionCobra Cardiotoxin ProteinsElapid VenomsEpithelial CellsFemaleGreen Fluorescent ProteinsHepatocytesKeratinocytesKeratinsLuminescent ProteinsMaleMiceMice, TransgenicMuscle CellsRadiation, IonizingRecombinasesRecombination, GeneticReverse Transcriptase Polymerase Chain ReactionStem CellsX ChromosomeY ChromosomeConceptsCell-cell fusionBone marrow-derived cellsCre/lox systemGreen fluorescent protein expressionFluorescent protein expressionEpithelial cellsDevelopmental plasticityLox systemCell fusionProtein expressionMarrow-derived cellsTransgenic miceCellsBone marrowFusionFusion requirementsPlasticityExpressionPlasticity of Bone Marrow–Derived Stem Cells
Grove JE, Bruscia E, Krause DS. Plasticity of Bone Marrow–Derived Stem Cells. Stem Cells 2004, 22: 487-500. PMID: 15277695, DOI: 10.1634/stemcells.22-4-487.Peer-Reviewed Original ResearchConceptsBone marrow stem cellsHematopoietic stem cellsStem cellsCell plasticityMesenchymal stem cellsStem cell plasticityGene expression profilesAdult stem cellsAdult bone marrow cellsMature lineagesAdult bone marrow stem cellsTissue of originExpression profilesMature cellsMarrow stem cellsBone marrow cellsNonhematopoietic tissuesMature phenotypePlasticityMarrow cellsCellsLineagesBone marrowPhenotypeTissue
2003
Plasticity of marrow-derived stem cells
Herzog EL, Chai L, Krause DS. Plasticity of marrow-derived stem cells. Blood 2003, 102: 3483-3493. PMID: 12893756, DOI: 10.1182/blood-2003-05-1664.Peer-Reviewed Original ResearchConceptsHematopoietic stem cellsStem cellsAdult stem cell plasticityMesenchymal stem cellsStem cell plasticityBone marrowMature blood cellsEndothelial cell progenitorsAdult bone marrowBone marrow subpopulationsMultiple mesenchymal tissuesCell plasticityMarrow-derived stem cellsCell progenitorsMarrow subpopulationsMultiple tissuesMature cellsConditions differentiationNeural cellsDifferentiationEpithelial cellsNonhematopoietic cellsTissue injuryGastrointestinal tractSkeletal muscleBone marrow stem cells contribute to repair of the ischemically injured renal tubule
Kale S, Karihaloo A, Clark PR, Kashgarian M, Krause DS, Cantley LG. Bone marrow stem cells contribute to repair of the ischemically injured renal tubule. Journal Of Clinical Investigation 2003, 112: 42-49. PMID: 12824456, PMCID: PMC162291, DOI: 10.1172/jci17856.Peer-Reviewed Original ResearchConceptsAcute tubular necrosisBone marrow stem cellsRenal tubulesMarrow stem cellsTubular necrosisRenal ischemiaTransient renal ischemiaAcute renal failureRenal tubular epithelial cellsStem cell infusionBlood urea nitrogenStem cellsTubular epithelial cellsMouse bone marrowRenal failureCell infusionHuman trialsTubular epitheliumBone marrowUrea nitrogenLin-Sca-1Adult mouse bone marrowNecrotic tubulesGreater riseEpithelial cells