2000
Determinants for cap trimethylation of the U2 small nuclear RNA are not conserved between Trypanosoma brucei and higher eukaryotic organisms
Günzl A, Bindereif A, Ullu E, Tschudi C. Determinants for cap trimethylation of the U2 small nuclear RNA are not conserved between Trypanosoma brucei and higher eukaryotic organisms. Nucleic Acids Research 2000, 28: 3702-3709. PMID: 11000261, PMCID: PMC110770, DOI: 10.1093/nar/28.19.3702.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBinding SitesCell LineConserved SequenceGuanosineMethylationMolecular Sequence DataMutationNucleic Acid ConformationPrecipitin TestsPromoter Regions, GeneticProtein BindingRegulatory Sequences, Nucleic AcidRibonucleoproteins, Small NuclearRNA CapsRNA, ProtozoanRNA, Small NuclearTransfectionTrypanosoma brucei bruceiConceptsCap trimethylationRibonucleoprotein particleEukaryotic organismsU2 snRNATrypanosoma bruceiU2 small nuclear RNA genesCap structureProtist parasite Trypanosoma bruceiSmall nuclear RNA genesCommon proteinsU2 small nuclear RNANuclear RNA genesHigher eukaryotic organismsRNA polymerase IIRNA polymerase IIISmall nuclear RNAMutant U2 snRNAsSet of proteinsParasite Trypanosoma bruceiSpliceosomal snRNPsRNP assemblyNuclear importPolymerase IIRNA genesSequence motifs
1995
Accurate Transcription of the Trypanosoma brucei U2 Small Nuclear RNA Gene in a Homologous Extract *
Günzl A, Tschudi C, Nakaar V, Ullu E. Accurate Transcription of the Trypanosoma brucei U2 Small Nuclear RNA Gene in a Homologous Extract *. Journal Of Biological Chemistry 1995, 270: 17287-17291. PMID: 7542235, DOI: 10.1074/jbc.270.29.17287.Peer-Reviewed Original ResearchConceptsIntragenic control regionRNA polymerase IIIControl regionPolymerase IIIU2 small nuclear RNA genesTranscription systemSmall nuclear RNA genesGene expressionInsect form trypanosomesNuclear RNA genesU2 snRNA genesSnRNA gene promotersB-box elementsFull transcriptional activitySeries of mutationsSnRNA genesRNA genesInsect formsRNA polymeraseGene promoterTrypanosoma bruceiCell-free extractsTransient expressionBox elementTranscriptional activity
1994
RNA polymerase III-mediated transcription of the trypanosome U2 small nuclear RNA gene is controlled by both intragenic and extragenic regulatory elements.
Fantoni A, Dare A, Tschudi C. RNA polymerase III-mediated transcription of the trypanosome U2 small nuclear RNA gene is controlled by both intragenic and extragenic regulatory elements. Molecular And Cellular Biology 1994, 14: 2021-2028. PMID: 8114733, PMCID: PMC358562, DOI: 10.1128/mcb.14.3.2021.Peer-Reviewed Original ResearchMeSH KeywordsAmanitinsAnimalsBase SequenceConsensus SequenceDNA, ProtozoanGenes, ProtozoanMolecular Sequence DataPromoter Regions, GeneticRegulatory Sequences, Nucleic AcidRNA Polymerase IIIRNA, Small NuclearSequence AlignmentSequence DeletionSequence Homology, Nucleic AcidTranscription, GeneticTrypanosomaConceptsU2 small nuclear RNA genesSmall nuclear RNA genesNuclear RNA genesU2 snRNA genesSnRNA genesRNA genesCis-acting regulatory sequencesRNA polymerase III transcriptsInsect form trypanosomesSnRNA gene expressionRNA polymerase IIInternal control regionRNA polymerase IIIPolymerase III transcriptsTranscription start siteIII-like enzymeTRNA genesPolymerase IIRNA polymeraseRegulatory sequencesU2 snRNAControl regionPolymerase IIIStart siteRegulatory elementsRNA Polymerase III-Mediated Transcription of the Trypanosome U2 Small Nuclear RNA Gene Is Controlled by both Intragenic and Extragenic Regulatory Elements
Fantoni A, Dare A, Tschudi C. RNA Polymerase III-Mediated Transcription of the Trypanosome U2 Small Nuclear RNA Gene Is Controlled by both Intragenic and Extragenic Regulatory Elements. Molecular And Cellular Biology 1994, 14: 2021-2028. DOI: 10.1128/mcb.14.3.2021-2028.1994.Peer-Reviewed Original ResearchU2 snRNA genesSnRNA genesU2 small nuclear RNA genesSmall nuclear RNA genesRNA polymerase III transcriptionRNA polymerase III-mediated transcriptionCis-acting regulatory sequencesPolymerase III transcriptionRNA polymerase IIIII-mediated transcriptionInternal control regionInsect form trypanosomesTranscription start siteTrimethyl cap structureTRNA genesIII transcriptionPolymerase IIU2 snRNARNA genesStart sitePromoter elementsRegulatory sequencesCoding regionElement transcriptionIntragenic sequencesRNA Polymerase III-Mediated Transcription of the Trypanosome U2 Small Nuclear RNA Gene Is Controlled by both Intragenic and Extragenic Regulatory Elements
Fantoni A, Dare A, Tschudi C. RNA Polymerase III-Mediated Transcription of the Trypanosome U2 Small Nuclear RNA Gene Is Controlled by both Intragenic and Extragenic Regulatory Elements. Molecular And Cellular Biology 1994, 14: 2021-2028. DOI: 10.1128/mcb.14.3.2021-2028.1994.Peer-Reviewed Original ResearchU2 small nuclear RNA genesSmall nuclear RNA genesNuclear RNA genesU2 snRNA genesSnRNA genesRNA genesRNA polymeraseCis-acting regulatory sequencesRNA polymerase III transcriptsInsect form trypanosomesSnRNA gene expressionRNA polymerase IIInternal control regionPolymerase III transcriptsTranscription start siteIII-like enzymeTRNA genesPolymerase IIRegulatory sequencesU2 snRNAControl regionStart siteRegulatory elementsPromoter elementsMutant constructs