2008
Differential Effects of Shear Stress and Cyclic Strain on Sp1 Phosphorylation by Protein Kinase Cζ Modulates Membrane Type 1–Matrix Metalloproteinase in Endothelial Cells
Kim JI, Cordova AC, Hirayama Y, Madri JA, Sumpio BE. Differential Effects of Shear Stress and Cyclic Strain on Sp1 Phosphorylation by Protein Kinase Cζ Modulates Membrane Type 1–Matrix Metalloproteinase in Endothelial Cells. Endothelium 2008, 15: 33-42. PMID: 18568943, PMCID: PMC2644408, DOI: 10.1080/10623320802092260.Peer-Reviewed Original ResearchConceptsSp1 phosphorylationMT1-MMP expressionPromoter sitesPKCzeta inhibitorProtein kinase CzetaAffinity of Sp1Egr-1 bindingProtein kinase CζExtracellular matrix remodelingEndothelial cell migrationSp1Cell migrationPhosphorylationMatrix remodelingProtein expressionCyclic strainExpressionMembrane typeEndothelial cellsKey roleCzetaInhibitorsCζMetalloproteinaseAffinity
2000
Translocation of PKC isoforms in bovine aortic smooth muscle cells exposed to strain
Han O, Takei T, Basson M, Sumpio B. Translocation of PKC isoforms in bovine aortic smooth muscle cells exposed to strain. Journal Of Cellular Biochemistry 2000, 80: 367-372. PMID: 11135367, DOI: 10.1002/1097-4644(20010301)80:3<367::aid-jcb100>3.0.co;2-2.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsAortic smooth muscle cellsBovine aortic smooth muscle cellsMuscle cellsPKC-alphaPKC isoformsCyclic strain resultsCycles/minCytosolic fractionProliferation responseParticulate fractionNF-kappaBProtein kinase C activityCollagen type ICellular proliferationSignificant stimulationImmunoblotting analysisKinase C activityType IPKC activityC activityStimulationIndividual PKC isoformsIsoforms
1998
Strain induces Caco-2 intestinal epithelial proliferation and differentiation via PKC and tyrosine kinase signals
Han O, Di Li G, Sumpio B, Basson M. Strain induces Caco-2 intestinal epithelial proliferation and differentiation via PKC and tyrosine kinase signals. American Journal Of Physiology 1998, 275: g534-g541. PMID: 9724266, DOI: 10.1152/ajpgi.1998.275.3.g534.Peer-Reviewed Original ResearchMolecular Basis for Tissue Expansion: Clinical Implications for the Surgeon
Takei T, Mills I, Arai K, Sumpio B. Molecular Basis for Tissue Expansion: Clinical Implications for the Surgeon. Plastic & Reconstructive Surgery 1998, 102: 247-258. PMID: 9655439, DOI: 10.1097/00006534-199807000-00044.Peer-Reviewed Original ResearchCyclooxygenase Expression in Bovine Aortic Endothelial Cells Exposed to Cyclic Strain
Kito H, Yokoyama C, Inoue H, Tanabe T, Nakajima N, Sumpio B. Cyclooxygenase Expression in Bovine Aortic Endothelial Cells Exposed to Cyclic Strain. Endothelium 1998, 6: 107-112. PMID: 9930644, DOI: 10.3109/10623329809072197.Peer-Reviewed Original ResearchConceptsAortic endothelial cellsBovine aortic endothelial cellsEndothelial cellsInduction of COXCOX-1 expressionCOX-2 promoter activityExposure of ECsPromoter activityCycles/minCOX-2Cyclooxygenase expressionCOX-1Weak inducerMRNA expressionExtent of inductionNorthern blot analysisBlot analysisTransient transfection experimentsLipopolysaccharideMinimal effectExpressionInductionTransfection experimentsCyclic strainCells
1997
Cyclic strain stimulates isoform‐specific PKC activation and translocation in cultured human keratinocytes
Takei T, Han O, Ikeda M, Male P, Mills I, Sumpio B. Cyclic strain stimulates isoform‐specific PKC activation and translocation in cultured human keratinocytes. Journal Of Cellular Biochemistry 1997, 67: 327-337. PMID: 9361188, DOI: 10.1002/(sici)1097-4644(19971201)67:3<327::aid-jcb5>3.0.co;2-y.Peer-Reviewed Original Research
1992
Protein kinase C is a mediator of the adaptation of vascular endothelial cells to cyclic strain in vitro.
Rosales OR, Sumpio BE. Protein kinase C is a mediator of the adaptation of vascular endothelial cells to cyclic strain in vitro. Surgery 1992, 112: 459-66. PMID: 1379381.Peer-Reviewed Original ResearchConceptsProtein kinase CActivation of PKCImmunocytochemical stainingPKC activityCyclic stretchEndothelial cell activationVascular endothelial cellsCycles/minSustained elevationCell activationFlexible-bottomed culture platesKinase CBovine aortic ECsECS resultsEndothelial cellsBlood vesselsAortic ECsEvidence of translocationSpecific PKC inhibitorEC proliferationCalphostin CEC growthHemodynamic forces