2001
Cyclic Stretch Induces the Expression of Vascular Endothelial Growth Factor in Vascular Smooth Muscle Cells
Smith JD, Davies N, Willis AI, Sumpio BE, Zilla P. Cyclic Stretch Induces the Expression of Vascular Endothelial Growth Factor in Vascular Smooth Muscle Cells. Endothelium 2001, 8: 41-48. PMID: 11409850, DOI: 10.3109/10623320109063156.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAorta, ThoracicCattleCells, CulturedCoculture TechniquesCulture MediaEndothelial Growth FactorsEndothelium, VascularGene Expression RegulationLymphokinesMuscle, Smooth, VascularPhosphorylationReceptor Protein-Tyrosine KinasesReceptors, Growth FactorReceptors, Vascular Endothelial Growth FactorStress, MechanicalVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsVasodilationConceptsVascular smooth muscle cellsVascular endothelial growth factorSmooth muscle cellsEndothelial growth factorExposure of VSMCsMuscle cellsGrowth factorMigration of ECsBovine vascular smooth muscle cellsSteady-state levelsCyclic stretchNormal endothelial cell functionEndothelial cell functionVEGF receptor KDRRelease of VEGFVEGF receptor phosphorylationVEGF secretionMRNA steady-state levelsVEGF receptorsReceptor KDRVEGF mRNACell functionReceptor phosphorylationSignificant increaseEC survival
1998
Understanding molecular biology.
Gahtan V, Peyman JA, Sumpio BE. Understanding molecular biology. Seminars In Vascular Surgery 1998, 11: 125-33. PMID: 9763112.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNAGene ExpressionGene Expression RegulationGenetic TechniquesHeart DiseasesHumansRNAVascular DiseasesInduction of interleukin (IL)‐1α and β gene expression in human keratinocytes exposed to repetitive strain: Their role in strain‐induced keratinocyte proliferation and morphological change
Takei T, Kito H, Du W, Mills I, Sumpio B. Induction of interleukin (IL)‐1α and β gene expression in human keratinocytes exposed to repetitive strain: Their role in strain‐induced keratinocyte proliferation and morphological change. Journal Of Cellular Biochemistry 1998, 69: 95-103. PMID: 9548558, DOI: 10.1002/(sici)1097-4644(19980501)69:2<95::aid-jcb1>3.0.co;2-p.Peer-Reviewed Original ResearchMeSH KeywordsAntibodiesCell DivisionCell SizeCells, CulturedGene Expression RegulationGrowth InhibitorsHumansInterleukin-1KeratinocytesMalePhenotypeStress, MechanicalConceptsIL-1 antibodyIL-1 alphaIL-1Keratinocyte proliferationIL-1 mRNA expressionKeratinocyte biologyIL-1 gene expressionInduction of interleukinIL-1 betaIL-1 expressionMorphological changesΒ gene expressionAutocrine regulationMRNA expressionBeta mRNAGene expressionBasal rateBasic cellular propertiesHuman keratinocytesAntibodiesKeratinocyte functionRepetitive strainNorthern blot analysisBlot analysisProliferation
1997
Gene Regulation by Mechanical Forces
Oluwole BO, Du W, Mills I, Sumpio BE. Gene Regulation by Mechanical Forces. Endothelium 1997, 5: 85-93. PMID: 9237042, DOI: 10.3109/10623329709079866.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell Adhesion MoleculesCell Culture TechniquesCells, CulturedEndothelinsEndothelium, VascularEpoprostenolFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGene Expression RegulationHemorheologyHumansMechanoreceptorsMembranes, ArtificialNitric OxideProtein-Tyrosine KinasesSignal TransductionStress, MechanicalTissue Plasminogen ActivatorTranscription FactorsTranscription, GeneticVacuumConceptsEndothelial cellsEndothelial cell functionCyclic strainVasoactive mediatorsSecond messenger cascadesNitric oxideBlood vesselsMechanical forcesCell functionMechanical deformationFlow of bloodMessenger cascadesLuminal surfaceSpecific response elementsPromoter geneResponse elementExternal forcesMediatorsCytoskeletal changesCellsForceProstacyclinExpressionTranscription levels
1995
Cyclic strain causes heterogeneous induction of transcription factors, AP-1, CRE binding protein and NF-kB, in endothelial cells: Species and vascular bed diversity
Du W, Mills I, Sumpio B. Cyclic strain causes heterogeneous induction of transcription factors, AP-1, CRE binding protein and NF-kB, in endothelial cells: Species and vascular bed diversity. Journal Of Biomechanics 1995, 28: 1485-1491. PMID: 8666588, DOI: 10.1016/0021-9290(95)00096-8.Peer-Reviewed Original ResearchMeSH KeywordsActivating Transcription Factor 2AnimalsAortaCattleCell DivisionCyclic AMP Response Element-Binding ProteinEndothelium, VascularEpoprostenolGene Expression RegulationGenes, Immediate-EarlyHumansNF-kappa BNuclear ProteinsPhenotypeProtein Kinase CSpecies SpecificityStress, MechanicalTime FactorsTranscription Factor AP-1Transcription FactorsUmbilical VeinsConceptsHuman umbilical vein endothelial cellsBovine aortic endothelial cellsActivator protein-1NF-kBEndothelial cellsVascular bedDifferent vascular bedsUmbilical vein endothelial cellsAortic endothelial cellsCultured endothelial cellsTranscription factor activator protein-1Vein endothelial cellsProstacyclin secretionStretch-induced activationImmediate early genesEC exposureNuclear factorTime pointsHeterogeneous inductionCAMP response elementProtein 1Biphasic mannerSignificant inductionDivergent effectsHuman aorta