2011
The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion
Chin A, Svejda B, Gustafsson B, Granlund A, Sandvik A, Timberlake A, Sumpio B, Pfragner R, Modlin I, Kidd M. The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion. AJP Gastrointestinal And Liver Physiology 2011, 302: g397-g405. PMID: 22038827, PMCID: PMC3287403, DOI: 10.1152/ajpgi.00087.2011.Peer-Reviewed Original ResearchMeSH KeywordsAcetamidesAdenosineAdenosine A2 Receptor AgonistsAdenosine A2 Receptor AntagonistsAdenosine-5'-(N-ethylcarboxamide)AdultAgedCell Line, TumorCells, CulturedColonCrohn DiseaseCyclic AMPCyclic AMP Response Element-Binding ProteinCyclic AMP-Dependent Protein KinasesEnterochromaffin CellsFemaleGene ExpressionHumansMaleMAP Kinase Kinase 1MAP Kinase Signaling SystemMechanotransduction, CellularMiddle AgedPhosphorylationProto-Oncogene Proteins c-aktPurinesReceptor, Adenosine A1Receptor, Adenosine A2AReceptor, Adenosine A2BReceptor, Adenosine A3SerotoninSignal TransductionStress, MechanicalTryptophan HydroxylaseVesicular Monoamine Transport ProteinsConceptsInflammatory bowel diseaseEC cellsCell functionEC cell functionMechanical stimulationDevelopment of agentsSecrete serotoninBowel diseaseIntracellular cAMP levelsGut motilityEnterochromaffin cellsAdenosine responsivenessReceptor agonistReceptor expressionHuman EC cellsSerotonin secretionAdenosine receptorsMRS1754CAMP productionSecretionCAMP levelsNECANeoplasiaMechanosensory cellsDisease
1998
Antiproliferative effect of elevated glucose in human microvascular endothelial cells
Kamal K, Du W, Mills I, Sumpio B. Antiproliferative effect of elevated glucose in human microvascular endothelial cells. Journal Of Cellular Biochemistry 1998, 71: 491-501. PMID: 9827695, DOI: 10.1002/(sici)1097-4644(19981215)71:4<491::aid-jcb4>3.0.co;2-p.Peer-Reviewed Original ResearchMeSH KeywordsCell DivisionCell LineCyclic AMPCyclic AMP Response Element-Binding ProteinCyclic AMP-Dependent Protein KinasesEndothelium, VascularEnzyme InhibitorsGlucoseHumansProtein Kinase CThionucleotidesTranscription Factor AP-1ConceptsMicrovascular endothelial cellsElevated glucoseHMEC-1Endothelial cellsAntiproliferative effectsDiabetic microangiopathyDiabetic foot ulcerationHuman dermal microvascular endothelial cellsCyclic AMP accumulationMicrovascular endothelial cell lineDermal microvascular endothelial cellsHuman microvascular endothelial cellsImmortalized human dermal microvascular endothelial cell lineHigh D-glucoseDeleterious effectsHuman dermal microvascular endothelial cell linePrimary cultured cellsEndothelial cell lineFoot ulcerationHMEC-1 proliferationProliferative responseAMP accumulationPathological complicationsMolecular pathophysiologyRp-cAMPS
1997
Regulation of tPA in endothelial cells exposed to cyclic strain: role of CRE, AP-2, and SSRE binding sites
Sumpio B, Chang R, Xu W, Wang X, Du W. Regulation of tPA in endothelial cells exposed to cyclic strain: role of CRE, AP-2, and SSRE binding sites. American Journal Of Physiology 1997, 273: c1441-c1448. PMID: 9374627, DOI: 10.1152/ajpcell.1997.273.5.c1441.Peer-Reviewed Original ResearchAnimalsAorta, ThoracicBase SequenceBinding SitesCattleCell CycleCell NucleusCells, CulturedChloramphenicol O-AcetyltransferaseCyclic AMP Response Element-Binding ProteinDNA-Binding ProteinsEndothelium, VascularGene Expression Regulation, EnzymologicHumansKineticsMutagenesis, Site-DirectedOligodeoxyribonucleotidesPromoter Regions, GeneticRecombinant Fusion ProteinsStress, MechanicalTime FactorsTissue Plasminogen ActivatorTranscription Factor AP-2Transcription FactorsTransfectionStrain activation of bovine aortic smooth muscle cell proliferation and alignment: Study of strain dependency and the role of protein kinase A and C signaling pathways
Mills I, Cohen C, Kamal K, Li G, Shin T, Du W, Sumpio B. Strain activation of bovine aortic smooth muscle cell proliferation and alignment: Study of strain dependency and the role of protein kinase A and C signaling pathways. Journal Of Cellular Physiology 1997, 170: 228-234. PMID: 9066778, DOI: 10.1002/(sici)1097-4652(199703)170:3<228::aid-jcp2>3.0.co;2-q.Peer-Reviewed Original ResearchMeSH KeywordsActivating Transcription Factor 2Adenylyl CyclasesAnimalsAortaCattleCell DivisionCells, CulturedCyclic AMPCyclic AMP Response Element-Binding ProteinCyclic AMP-Dependent Protein KinasesEndothelium, VascularLeucine ZippersMuscle, Smooth, VascularProtein Kinase CSignal TransductionStress, MechanicalTranscription FactorsConceptsBovine aortic SMCSMC proliferationCAMP response elementAortic SMCsAC/cAMP/PKAAortic smooth muscle cell proliferationSmooth muscle cell proliferationProtein levelsCyclic AMP accumulationMuscle cell proliferationSmooth muscle cell phenotypeExposure of SMCAdenylate cyclase activityMuscle cell phenotypeAMP accumulationInhibition of PKACAMP/PKACyclic AMP pathwayMultifactorial natureProtein kinase C activityCyclase activityProtein kinase A (PKA) activityLack of involvementEnhanced proliferationCell proliferationActivation of the Adenylyl Cyclase/Cyclic AMP/Protein Kinase A Pathway in Endothelial Cells Exposed to Cyclic Strain
Cohen C, Mills I, Du W, Kamal K, Sumpio B. Activation of the Adenylyl Cyclase/Cyclic AMP/Protein Kinase A Pathway in Endothelial Cells Exposed to Cyclic Strain. Experimental Cell Research 1997, 231: 184-189. PMID: 9056425, DOI: 10.1006/excr.1996.3450.Peer-Reviewed Original ResearchAdenylyl CyclasesAnimalsCattleCyclic AMPCyclic AMP Response Element-Binding ProteinCyclic AMP-Dependent Protein KinasesEndothelium, VascularEnzyme ActivationStress, Mechanical
1995
Cyclic strain causes heterogeneous induction of transcription factors, AP-1, CRE binding protein and NF-kB, in endothelial cells: Species and vascular bed diversity
Du W, Mills I, Sumpio B. Cyclic strain causes heterogeneous induction of transcription factors, AP-1, CRE binding protein and NF-kB, in endothelial cells: Species and vascular bed diversity. Journal Of Biomechanics 1995, 28: 1485-1491. PMID: 8666588, DOI: 10.1016/0021-9290(95)00096-8.Peer-Reviewed Original ResearchMeSH KeywordsActivating Transcription Factor 2AnimalsAortaCattleCell DivisionCyclic AMP Response Element-Binding ProteinEndothelium, VascularEpoprostenolGene Expression RegulationGenes, Immediate-EarlyHumansNF-kappa BNuclear ProteinsPhenotypeProtein Kinase CSpecies SpecificityStress, MechanicalTime FactorsTranscription Factor AP-1Transcription FactorsUmbilical VeinsConceptsHuman umbilical vein endothelial cellsBovine aortic endothelial cellsActivator protein-1NF-kBEndothelial cellsVascular bedDifferent vascular bedsUmbilical vein endothelial cellsAortic endothelial cellsCultured endothelial cellsTranscription factor activator protein-1Vein endothelial cellsProstacyclin secretionStretch-induced activationImmediate early genesEC exposureNuclear factorTime pointsHeterogeneous inductionCAMP response elementProtein 1Biphasic mannerSignificant inductionDivergent effectsHuman aorta