2018
Regulation of Yes-Associated Protein by Laminar Flow
Chitragari G, Shalaby SY, Sumpio BJ, Kurita J, Sumpio BE. Regulation of Yes-Associated Protein by Laminar Flow. Annals Of Vascular Surgery 2018, 52: 183-191. PMID: 29758328, DOI: 10.1016/j.avsg.2018.03.002.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingCell ShapeCells, CulturedCytoskeletonDown-RegulationHuman Umbilical Vein Endothelial CellsHumansMechanotransduction, CellularPhosphoproteinsPhosphorylationProteolysisPulsatile FlowRegional Blood FlowStress, MechanicalTime FactorsTranscription FactorsYAP-Signaling ProteinsConceptsHuman umbilical vein endothelial cellsTotal YAPExposure of HUVECsRelease of mediatorsYAP levelsSignificant decreaseUbiquitin-proteasome system inhibitorsUmbilical vein endothelial cellsEndothelial cell proliferationVein endothelial cellsVascular healthSystem inhibitorsYes-Associated ProteinDisease modulationIntimal hyperplasiaDisturbed flowEndothelial cellsBlood vesselsDisease statesPotential targetCell proliferationProteasome-independent mechanismExposureKey regulatorPYAP
2014
Effect of Pulsatile and Continuous Flow on Yes-Associated Protein
Chitragari G, Shalaby SY, Sumpio BJ, Sumpio BE. Effect of Pulsatile and Continuous Flow on Yes-Associated Protein. International Journal Of Angiology 2014, 23: 183-186. PMID: 25317030, PMCID: PMC4169103, DOI: 10.1055/s-0034-1376865.Peer-Reviewed Original ResearchHuman umbilical vein endothelial cellsContinuous forward flowTotal YAPPhosphorylated YAPEndothelial cellsPathogenesis of atherosclerosisEffects of pulsatileRole of YAPUmbilical vein endothelial cellsVein endothelial cellsYes-Associated ProteinMild decreaseFlow chamber systemFibronectin-coated glass slidesParallel plate flow chamber systemSignificant decreaseLonger durationSignificant differencesAtherosclerosisDifferential effectsLevel of phosphorylationForward flowMinutesYAPPFF