2012
Olive Oil Polyphenols Differentially Inhibit Smooth Muscle Cell Proliferation through a G1/S Cell Cycle Block Regulated by ERK1/2
Abe R, Beckett J, Abe R, Nixon A, Rochier A, Yamashita N, Sumpio B. Olive Oil Polyphenols Differentially Inhibit Smooth Muscle Cell Proliferation through a G1/S Cell Cycle Block Regulated by ERK1/2. International Journal Of Angiology 2012, 21: 069-076. PMID: 23730132, PMCID: PMC3444010, DOI: 10.1055/s-0032-1315630.Peer-Reviewed Original ResearchSmooth muscle cell proliferationMuscle cell proliferationCell cycle blockOlive oil polyphenolsCell cycle analysisDay 1SMC proliferationG1/S cell cycle blockCycle blockOil polyphenolsRisk of atherosclerosisCell proliferationOLE groupControl groupFlow cytometryVascular SMCsWestern blottingDays of exposureS phaseCycle analysisERK1/2 activationNumber of cellsCell populationsOlive oilG1 phase
2011
Olive Oil Polyphenol Oleuropein Inhibits Smooth Muscle Cell Proliferation
Abe R, Beckett J, Abe R, Nixon A, Rochier A, Yamashita N, Sumpio B. Olive Oil Polyphenol Oleuropein Inhibits Smooth Muscle Cell Proliferation. European Journal Of Vascular And Endovascular Surgery 2011, 41: 814-820. PMID: 21333557, DOI: 10.1016/j.ejvs.2010.12.021.Peer-Reviewed Original ResearchConceptsSmooth muscle cell proliferationMuscle cell proliferationCell cycle analysisSMC proliferationCell proliferationInhibits Smooth Muscle Cell ProliferationVascular smooth muscle cell proliferationCoronary artery diseaseNon-treated groupExtracellular signal-regulated kinase 1/2 activationCardiovascular mortalityArtery diseaseKinase 1/2 activationCell cycle blockG1-S phaseMediterranean dietDay 1Oleuropein treatmentGrowth of SMCsG1 phase regulatorsFlow cytometryVascular SMCsPresence of oleuropeinBeneficial effectsCycle analysis
2005
Resveratrol inhibits vascular smooth muscle cell proliferation and induces apoptosis
Poussier B, Cordova AC, Becquemin JP, Sumpio BE. Resveratrol inhibits vascular smooth muscle cell proliferation and induces apoptosis. Journal Of Vascular Surgery 2005, 42: 1190-1190.e14. PMID: 16376213, DOI: 10.1016/j.jvs.2005.08.014.Peer-Reviewed Original ResearchMeSH KeywordsAngiogenesis InhibitorsAnimalsAnimals, NewbornAntioxidantsAorta, ThoracicApoptosisBlotting, WesternCattleCell ProliferationCells, CulturedDNADose-Response Relationship, DrugFlow CytometryIn Situ Nick-End LabelingIn Vitro TechniquesMuscle, Smooth, VascularProliferating Cell Nuclear AntigenResveratrolStilbenesConceptsSmooth muscle cell proliferationMuscle cell proliferationVascular smooth muscle cell proliferationDose-dependent mannerSMC proliferationCell proliferationG1-S phaseBeneficial effectsFrench paradoxDose-dependent apoptotic effectTerminal deoxynucleotidyl transferase-mediated dUTP-biotin nickTransferase-mediated dUTP-biotin nickCardiovascular death ratesRed wine intakeRed wine consumptionEffects of resveratrolAortic SMC proliferationEnd labeling stainingVascular SMC proliferationDUTP-biotin nickMajor polyphenol componentHealthy life styleCell cycle analysisCardiovascular mortalityFlow-activated cell sortingSustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway
Asada H, Paszkowiak J, Teso D, Alvi K, Thorisson A, Frattini JC, Kudo FA, Sumpio BE, Dardik A. Sustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway. Journal Of Vascular Surgery 2005, 42: 772-780. PMID: 16242567, DOI: 10.1016/j.jvs.2005.05.046.Peer-Reviewed Original ResearchConceptsOrbital shear stressPresence of PD98059Synthetic phenotypeERK1/2 pathwayExtracellular signal-regulated proteinSMC proliferationSmooth muscle cell proliferationMuscle cell proliferationPathway inhibitor PD98059Cell proliferationExtracellular signal-regulated protein kinase 1/2 (ERK1/2) phosphorylationKinase 1/2 phosphorylationPresence of SB203580Bovine SMCsSMC phenotypeInhibitor PD98059Inhibitor SB203580Contractile phenotypePD98059Long-term culturePhenotypeCell nuclear antigenExpression of markersPathwaySB203580Homocysteine promotes p38-dependent chemotaxis in bovine aortic smooth muscle cells
Akasaka K, Akasaka N, Di Luozzo G, Sasajima T, Sumpio BE. Homocysteine promotes p38-dependent chemotaxis in bovine aortic smooth muscle cells. Journal Of Vascular Surgery 2005, 41: 517-522. PMID: 15838488, DOI: 10.1016/j.jvs.2004.12.043.Peer-Reviewed Original ResearchConceptsEffect of homocysteineMigration of SMCsLevels of homocysteineProgressive intimal thickeningAortic smooth muscle cellsSmooth muscle cell migrationBovine aortic smooth muscle cellsPotential therapeutic implicationsSmooth muscle cellsP38 activationExposure of SMCMuscle cell migrationSMC chemotaxisRisk factorsSelective blockadeIntimal thickeningTherapeutic implicationsP38-dependent pathwaySMC proliferationBoyden chamberChemotactic potentialMuscle cellsHomocysteineFetal bovine serumP38 inhibitor
2003
Involvement of S6 kinase and p38 mitogen activated protein kinase pathways in strain‐induced alignment and proliferation of bovine aortic smooth muscle cells
Li W, Chen Q, Mills I, Sumpio BE. Involvement of S6 kinase and p38 mitogen activated protein kinase pathways in strain‐induced alignment and proliferation of bovine aortic smooth muscle cells. Journal Of Cellular Physiology 2003, 195: 202-209. PMID: 12652647, DOI: 10.1002/jcp.10230.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell DivisionCells, CulturedDNA-Binding ProteinsEnzyme InhibitorsImidazolesImmunosuppressive AgentsMAP Kinase Signaling SystemMitogen-Activated Protein KinasesMuscle, Smooth, VascularMyocytes, Smooth MuscleP38 Mitogen-Activated Protein KinasesPyridinesRegional Blood FlowRibosomal Protein S6 KinasesSirolimusStress, MechanicalTranscription FactorsConceptsAortic smooth muscle cellsBovine aortic smooth muscle cellsKinase pathwaySmooth muscle cell phenotypeSmooth muscle cellsStrain-induced proliferationBovine aortic SMCS6 kinaseMuscle cell phenotypeSpecific inhibitorProliferation of SMCsActivation of p38SMC proliferationAortic SMCsMuscle cellsSMC alignment
2002
Photochemotherapy of vascular cells with 8‐methoxypsoralen and visible light: differential effects on endothelial and smooth muscle cells
Lee DM, Gasparro FP, Wang XJ, Kopec C, DeLeo K, Sumpio BE. Photochemotherapy of vascular cells with 8‐methoxypsoralen and visible light: differential effects on endothelial and smooth muscle cells. Photodermatology Photoimmunology & Photomedicine 2002, 18: 244-252. PMID: 12390666, DOI: 10.1034/j.1600-0781.2002.02770.x.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsEndothelial cellsPercutaneous transluminal coronary angioplastyMuscle cellsTransluminal coronary angioplastyLong-term efficacyAortic smooth muscle cellsBovine aortic smooth muscle cellsDose-dependent fashionReversible inhibitionCoronary angioplastyIntermediate doseLow doseHigh doseSMC proliferationCell countVascular cellsDoseProliferation of ECsEC proliferationDifferential effectsProliferationCellular migrationPresent studySignificant effectInhibition of vascular smooth muscle cell proliferation with red wine and red wine polyphenols
Araim O, a J, Waterhouse AL, Sumpio BE. Inhibition of vascular smooth muscle cell proliferation with red wine and red wine polyphenols. Journal Of Vascular Surgery 2002, 35: 1226-1232. PMID: 12042735, DOI: 10.1067/mva.2002.124358.Peer-Reviewed Original ResearchConceptsRed wine polyphenol extractVascular SMC proliferationRed wine polyphenolsVascular smooth muscle cell proliferationSmooth muscle cell proliferationMuscle cell proliferationSMC proliferationAtherosclerotic diseaseBeneficial effectsWine polyphenolsVascular SMCsTrypan blue exclusion studiesDealcoholized red wineRed wine consumptionCell proliferationPolyphenol extractDose-dependent fashionInhibits SMC proliferationPotential beneficial effectsObserved beneficial effectsBovine aortic SMCDehydrogenase cytotoxicityAortic SMCsInhibitory effectRed wine
1998
The differential effect of contrast agents on endothelial cell and smooth muscle cell growth in vitro
Sawmiller C, Powell R, Quader M, Dudrick S, Sumpio B. The differential effect of contrast agents on endothelial cell and smooth muscle cell growth in vitro. Journal Of Vascular Surgery 1998, 27: 1128-1140. PMID: 9652475, DOI: 10.1016/s0741-5214(98)70015-1.Peer-Reviewed Original ResearchConceptsNonionic contrastEndothelial cellsIonic contrastSmooth muscle cell proliferationSignificant decreaseSmooth muscle cell growthNonionic contrast agentsCultured bovine aorta endothelial cellsMuscle cell proliferationBrief exposureMuscle cell growthRole of osmolalityContrast exposureEC injuryContrast agentsAorta endothelial cellsLength of exposureDay 3SMC proliferationOsmolar effectsBovine aorta endothelial cellsEffect of contrastCell proliferationConfluent endothelial cellsOsmolar solution
1997
Strain activation of bovine aortic smooth muscle cell proliferation and alignment: Study of strain dependency and the role of protein kinase A and C signaling pathways
Mills I, Cohen C, Kamal K, Li G, Shin T, Du W, Sumpio B. Strain activation of bovine aortic smooth muscle cell proliferation and alignment: Study of strain dependency and the role of protein kinase A and C signaling pathways. Journal Of Cellular Physiology 1997, 170: 228-234. PMID: 9066778, DOI: 10.1002/(sici)1097-4652(199703)170:3<228::aid-jcp2>3.0.co;2-q.Peer-Reviewed Original ResearchMeSH KeywordsActivating Transcription Factor 2Adenylyl CyclasesAnimalsAortaCattleCell DivisionCells, CulturedCyclic AMPCyclic AMP Response Element-Binding ProteinCyclic AMP-Dependent Protein KinasesEndothelium, VascularLeucine ZippersMuscle, Smooth, VascularProtein Kinase CSignal TransductionStress, MechanicalTranscription FactorsConceptsBovine aortic SMCSMC proliferationCAMP response elementAortic SMCsAC/cAMP/PKAAortic smooth muscle cell proliferationSmooth muscle cell proliferationProtein levelsCyclic AMP accumulationMuscle cell proliferationSmooth muscle cell phenotypeExposure of SMCAdenylate cyclase activityMuscle cell phenotypeAMP accumulationInhibition of PKACAMP/PKACyclic AMP pathwayMultifactorial natureProtein kinase C activityCyclase activityProtein kinase A (PKA) activityLack of involvementEnhanced proliferationCell proliferation
1994
Inhibition of smooth muscle cell proliferation by visible light-activated psoralen.
Sumpio BE, Li G, Deckelbaum LI, Gasparro FP. Inhibition of smooth muscle cell proliferation by visible light-activated psoralen. Circulation Research 1994, 75: 208-213. PMID: 8033334, DOI: 10.1161/01.res.75.2.208.Peer-Reviewed Original Research
1993
Control of smooth muscle cell proliferation by psoralen photochemotherapy.
Sumpio B, Phan S, Gasparro F, Deckelbaum L. Control of smooth muscle cell proliferation by psoralen photochemotherapy. Journal Of Vascular Surgery 1993, 17: 1010-6; discussion 1016-8. PMID: 8505779, DOI: 10.1067/mva.1993.45747.Peer-Reviewed Original ResearchConceptsSmooth muscle cell proliferationMuscle cell proliferationSMC proliferationPsoralen photochemotherapyPeripheral vascular diseaseLong-term therapyCell proliferationInhibits SMC proliferationTrypan blue exclusionBypass graftDistal anastomosisBalloon angioplastyUVA photochemotherapyVascular diseaseIntimal hyperplasiaGrowth of SMCsBlue exclusionPhotochemotherapySMC viabilityTime courseProliferationUVADNA synthesisDaysAngioplastyControl of smooth muscle cell proliferation by psoralen photochemotherapy
Sumpio B, Phan S, Gasparro F, Deckelbaum L. Control of smooth muscle cell proliferation by psoralen photochemotherapy. Journal Of Vascular Surgery 1993, 17: 1010-1018. DOI: 10.1016/0741-5214(93)90671-8.Peer-Reviewed Original ResearchConceptsSmooth muscle cell proliferationMuscle cell proliferationSMC proliferationPsoralen photochemotherapyPeripheral vascular diseaseLong-term therapyCell proliferationInhibits SMC proliferationTrypan blue exclusionBypass graftDistal anastomosisBalloon angioplastyUVA photochemotherapyVascular diseaseIntimal hyperplasiaGrowth of SMCsBlue exclusionPhotochemotherapySMC viabilityTime courseProliferationUVADNA synthesisDaysAngioplasty