1995
Cyclic strain upregulates nitric oxide synthase in cultured bovine aortic endothelial cells.
Awolesi MA, Sessa WC, Sumpio BE. Cyclic strain upregulates nitric oxide synthase in cultured bovine aortic endothelial cells. Journal Of Clinical Investigation 1995, 96: 1449-1454. PMID: 7544806, PMCID: PMC185768, DOI: 10.1172/jci118181.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseAortic endothelial cellsNitric oxide synthaseBovine aortic endothelial cellsCultured bovine aortic endothelial cellsEndothelial cellsOxide synthaseENOS transcriptsRegulation of eNOSENOS gene expressionBlot analysisHemodynamic forcesWestern blot analysisENOS levelsCycles/minImmunohistochemical stainingNitric oxideProtein levelsNorthern blot analysisNuclear runoff transcription assaysVivoCellsRunoff transcription assaysCytosolic RNAExpression
1994
Cyclic strain increases endothelial nitric oxide synthase activity.
Awolesi MA, Widmann MD, Sessa WC, Sumpio BE. Cyclic strain increases endothelial nitric oxide synthase activity. Surgery 1994, 116: 439-44; discussion 444-5. PMID: 7519368.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseRegulation of eNOSCultured bovine aortic endothelial cellsENOS activityAortic endothelial cellsEndothelial cellsBovine aortic endothelial cellsEndothelial nitric oxide synthase activityNitric oxideNitric oxide synthase activityOxide synthase activityNitric oxide synthaseNitric oxide productionHemodynamic forcesRelaxing factorsGreiss reactionCycles/minOxide synthaseENOS geneMedium of ECOxide productionCycles/minuteFunctional activityStrain regimensSynthase activity