2017
Shear Stress Induces Change in Extracellular Signal-Regulated Kinase 5 Levels with Sustained Activation under Disturbed and Continuous Laminar Flow
Shalaby SY, Chitragari G, Sumpio BJ, Sumpio BE. Shear Stress Induces Change in Extracellular Signal-Regulated Kinase 5 Levels with Sustained Activation under Disturbed and Continuous Laminar Flow. International Journal Of Angiology 2017, 26: 109-115. PMID: 28566937, PMCID: PMC5446257, DOI: 10.1055/s-0037-1599057.Peer-Reviewed Original ResearchExtracellular signal-regulated kinase 5Pathogenesis of atherosclerosisExposure of HUVECERK5 activationVascular dysfunctionPattern of activationFro flowVascular remodelingConfluent HUVECsHours of exposurePhospho-ERK5Endothelial integrityActivation of ERK5Kinase 5Sustained activationEnd of experimentationERK5 phosphorylationHUVECActivationHoursExposureTransient phosphorylationCell lysatesDisturbed flow conditionsParallel plate flow chamber
2014
Effect of Pulsatile and Continuous Flow on Yes-Associated Protein
Chitragari G, Shalaby SY, Sumpio BJ, Sumpio BE. Effect of Pulsatile and Continuous Flow on Yes-Associated Protein. International Journal Of Angiology 2014, 23: 183-186. PMID: 25317030, PMCID: PMC4169103, DOI: 10.1055/s-0034-1376865.Peer-Reviewed Original ResearchHuman umbilical vein endothelial cellsContinuous forward flowTotal YAPPhosphorylated YAPEndothelial cellsPathogenesis of atherosclerosisEffects of pulsatileRole of YAPUmbilical vein endothelial cellsVein endothelial cellsYes-Associated ProteinMild decreaseFlow chamber systemFibronectin-coated glass slidesParallel plate flow chamber systemSignificant decreaseLonger durationSignificant differencesAtherosclerosisDifferential effectsLevel of phosphorylationForward flowMinutesYAPPFF
2003
Shear stress stimulated endothelial cell derived PDGF and IL-1 alpha both stimulate SMC chemotaxis via the MAPK pathway
Dardik A, Yamashita A, Aziz F, Paszkowiak J, Asada H, Sumpio B. Shear stress stimulated endothelial cell derived PDGF and IL-1 alpha both stimulate SMC chemotaxis via the MAPK pathway. Journal Of Surgical Research 2003, 114: 249. DOI: 10.1016/j.jss.2003.08.159.Peer-Reviewed Original ResearchPlatelet-derived growth factor-BBIL-1SMC migrationEndothelial cellsMAPK inhibitor PD98059Pathogenesis of atherosclerosisSMC chemotaxisSmooth muscle cell migrationIL-1 alphaInterleukin-1 alphaSimilar degreeInhibitor PD98059Muscle cell migrationMAPK pathwayHemodynamic forcesGrowth factor-BBAortic endothelial cellsSS stimulationBovine aortic endothelial cellsArterial levelsNeointimal hyperplasiaParacrine mechanismsSMC mitogenBoyden chamberSoluble factors