2005
Sustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway
Asada H, Paszkowiak J, Teso D, Alvi K, Thorisson A, Frattini JC, Kudo FA, Sumpio BE, Dardik A. Sustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway. Journal Of Vascular Surgery 2005, 42: 772-780. PMID: 16242567, DOI: 10.1016/j.jvs.2005.05.046.Peer-Reviewed Original ResearchConceptsOrbital shear stressPresence of PD98059Synthetic phenotypeERK1/2 pathwayExtracellular signal-regulated proteinSMC proliferationSmooth muscle cell proliferationMuscle cell proliferationPathway inhibitor PD98059Cell proliferationExtracellular signal-regulated protein kinase 1/2 (ERK1/2) phosphorylationKinase 1/2 phosphorylationPresence of SB203580Bovine SMCsSMC phenotypeInhibitor PD98059Inhibitor SB203580Contractile phenotypePD98059Long-term culturePhenotypeCell nuclear antigenExpression of markersPathwaySB203580Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1α
Dardik A, Yamashita A, Aziz F, Asada H, Sumpio BE. Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1α. Journal Of Vascular Surgery 2005, 41: 321-331. PMID: 15768016, DOI: 10.1016/j.jvs.2004.11.016.Peer-Reviewed Original ResearchConceptsSignal transduction pathwaysPlatelet-derived growth factorSMC chemotaxisCell signal transduction pathwaysMitogen-activated protein kinase pathwaySmooth muscle cell chemotaxisERK1/2 phosphorylationPDGF-BBExtracellular signal-regulated protein kinase 1/2Pathway inhibitor PD98059Protein kinase pathwayEndothelial cellsERK1/2 signal transduction pathwayProtein kinase 1/2Vascular smooth muscle cell migrationBovine aortic endothelial cellsKinase pathwayInhibitor PD98059Smooth muscle cell migrationHemodynamic forcesKinase 1/2Platelet-derived growthMuscle cell migrationWestern blot analysisTarget pathways
2003
Shear stress stimulated endothelial cell derived PDGF and IL-1 alpha both stimulate SMC chemotaxis via the MAPK pathway
Dardik A, Yamashita A, Aziz F, Paszkowiak J, Asada H, Sumpio B. Shear stress stimulated endothelial cell derived PDGF and IL-1 alpha both stimulate SMC chemotaxis via the MAPK pathway. Journal Of Surgical Research 2003, 114: 249. DOI: 10.1016/j.jss.2003.08.159.Peer-Reviewed Original ResearchPlatelet-derived growth factor-BBIL-1SMC migrationEndothelial cellsMAPK inhibitor PD98059Pathogenesis of atherosclerosisSMC chemotaxisSmooth muscle cell migrationIL-1 alphaInterleukin-1 alphaSimilar degreeInhibitor PD98059Muscle cell migrationMAPK pathwayHemodynamic forcesGrowth factor-BBAortic endothelial cellsSS stimulationBovine aortic endothelial cellsArterial levelsNeointimal hyperplasiaParacrine mechanismsSMC mitogenBoyden chamberSoluble factors
2002
SPHINGOSINE-1-PHOSPHATE STIMULATES HUMAN CACO-2 INTESTINAL EPITHELIAL PROLIFERATION VIA p38 ACTIVATION AND ACTIVATES ERK BY AN INDEPENDENT MECHANISM
THAMILSELVAN V, LI W, SUMPIO BE, BASSON MD. SPHINGOSINE-1-PHOSPHATE STIMULATES HUMAN CACO-2 INTESTINAL EPITHELIAL PROLIFERATION VIA p38 ACTIVATION AND ACTIVATES ERK BY AN INDEPENDENT MECHANISM. In Vitro Cellular & Developmental Biology - Animal 2002, 38: 246-253. PMID: 12197778, DOI: 10.1290/1071-2690(2002)038<0246:spshci>2.0.co;2.Peer-Reviewed Original ResearchConceptsExtracellular signal-regulated kinases 1Mitogen-activated protein kinaseMAP kinase kinaseCaco-2 proliferationMAPK activationHuman intestinal epithelial proliferationP38 activationCell typesSignal-regulated kinases 1Role of ERKMitogenic effectCaco-2 intestinal epithelial cellsIntracellular second messengerMEK inhibitionP38 MAPK activationCancer cell invasionKinase kinaseHuman Caco-2 intestinal epithelial cellsProtein kinaseStimulation of proliferationCell motilityIntestinal epithelial cell proliferationInhibitor PD98059ERK2ERK activation