2011
Cyclic Strain Delays the Expression of Tissue Factor Induced by Thrombin in Human Umbilical Vein Endothelial Cells
Yamashita N, Abe R, Nixon A, Rochier A, Madri J, Sumpio B. Cyclic Strain Delays the Expression of Tissue Factor Induced by Thrombin in Human Umbilical Vein Endothelial Cells. International Journal Of Angiology 2011, 20: 157-166. PMID: 22942631, PMCID: PMC3331651, DOI: 10.1055/s-0031-1284475.Peer-Reviewed Original ResearchHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsVein endothelial cellsExtracellular signal-regulated protein kinase (ERK) inhibitorsProtein kinase inhibitorsEndothelial cellsEgr-1 levelsTF expressionERK activityERK inhibitorThrRNA expressionP38Messenger RNA expressionTF mRNA expressionCyclic strainCulture conditionsExpression of TFKinase inhibitorsExpressionStationary culture conditionsTissue factor expressionFactor expressionCellsMRNA expression
2010
The Effects of Freezing versus Supercooling on Vascular Cells: Implications for Balloon Cryoplasty
Basco MT, Yiu WK, Cheng SW, Sumpio BE. The Effects of Freezing versus Supercooling on Vascular Cells: Implications for Balloon Cryoplasty. Journal Of Vascular And Interventional Radiology 2010, 21: 910-915. PMID: 20417120, PMCID: PMC2878641, DOI: 10.1016/j.jvir.2010.02.016.Peer-Reviewed Original Research
2008
QS163. Differential Effects on Endothelial Cells and Smooth Muscle Cells After Freezing and Rewarming
Basco M, Yiu W, Sumpio B. QS163. Differential Effects on Endothelial Cells and Smooth Muscle Cells After Freezing and Rewarming. Journal Of Surgical Research 2008, 144: 332. DOI: 10.1016/j.jss.2007.12.405.Peer-Reviewed Original Research
2006
p38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress
Kadohama T, Akasaka N, Nishimura K, Hoshino Y, Sasajima T, Sumpio BE. p38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress. Endothelium 2006, 13: 43-50. PMID: 16885066, DOI: 10.1080/10623320600660219.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesAtherosclerosisBlood PressureBlood VesselsCattleCell PolarityCell ShapeCells, CulturedDose-Response Relationship, DrugEndothelial CellsEnzyme ActivationEnzyme InhibitorsHemodynamicsMAP Kinase Signaling SystemP38 Mitogen-Activated Protein KinasesPhosphorylationRegional Blood FlowStress, MechanicalTime FactorsConceptsStatic endothelial cellsFluid shear stressP38 MAPKP38 Mitogen-Activated Protein Kinase ActivationMitogen-Activated Protein Kinase ActivationEndothelial cellsProtein kinase activationP38 MAPK inhibitor SB-203580Inhibitor SB 203580Cell alignmentProtein kinaseBovine aortic endothelial cellsLaminar shear stressKinase activationSB 203580P38 mitogenCultured bovine aortic endothelial cellsEC elongationAortic endothelial cellsMAPKCellsElongationActivationMorphometric analysisKinase
2005
In–Vitro Model for Evaluation of the Effects of Supercooling and Re-warming on Vascular Cells
Yiu W, Aruny J, Cheng S, Sumpio B. In–Vitro Model for Evaluation of the Effects of Supercooling and Re-warming on Vascular Cells. International Journal Of Angiology 2005, 14: 237-241. DOI: 10.1007/s00547-005-2055-5.Peer-Reviewed Original ResearchInitial technical success rateEndothelial cellsRe-warming phasePeripheral vascular diseaseTransluminal balloon angioplastyRate of restenosisTechnical success rateTUNEL-positive cellsSmooth muscle cellsBalloon angioplastyVascular diseaseEndothelial cell survivalMuscle cellsVascular cellsVitro modelVessel wallSuccess rateUnderlying mechanismRestenosisCell survivalCellsCellular responsesCritical roleAngioplastyIn–Vitro model for evaluation of the effects of supercooling and re-warming on vascular cells
Yiu W, Aruny J, Cheng S, Sumpio B. In–Vitro model for evaluation of the effects of supercooling and re-warming on vascular cells. International Journal Of Angiology 2005, 237-241. DOI: 10.1055/s-0031-1276516.Peer-Reviewed Original ResearchInitial technical success rateEndothelial cellsRe-warming phasePeripheral vascular diseaseTransluminal balloon angioplastyRate of restenosisTechnical success rateTUNEL-positive cellsSmooth muscle cellsBalloon angioplastyVascular diseaseEndothelial cell survivalMuscle cellsVascular cellsVitro modelVessel wallSuccess rateUnderlying mechanismRestenosisCell survivalCellsCellular responsesCritical roleAngioplasty
2004
Cell signalling in vascular cells exposed to cyclic strain: the emerging role of protein phosphatases
Lee T, Sumpio BE. Cell signalling in vascular cells exposed to cyclic strain: the emerging role of protein phosphatases. Biotechnology And Applied Biochemistry 2004, 39: 129-139. PMID: 15032733, DOI: 10.1042/ba20030104.Peer-Reviewed Original ResearchConceptsPhosphorylation stateSerine/threonine residuesProtein phosphatase type 2AAbnormal phosphorylation stateCritical cellular functionsPhosphorylation of tyrosineRole of phosphatasesVascular cellsRole of proteinsThreonine residuesCellular functionsProtein kinaseIntracellular proteinsCellular growthHuman diseasesProteinPhosphataseType 2AEndothelial cellsCellsCardiac hypertrophyKinasePhosphorylationHaemodynamic forcesRole
2003
Fibronectin blocks p38 and jnk activation by cyclic strain in Caco-2 cells
Zhang J, Li W, Sumpio BE, Basson MD. Fibronectin blocks p38 and jnk activation by cyclic strain in Caco-2 cells. Biochemical And Biophysical Research Communications 2003, 306: 746-749. PMID: 12810082, DOI: 10.1016/s0006-291x(03)01044-1.Peer-Reviewed Original ResearchConceptsIntestinal epithelial responsesIntestinal epithelial proliferationCaco-2 intestinal epithelial cellsIntestinal epithelial cellsHuman Caco-2 intestinal epithelial cellsCaco-2 cellsPlasma fibronectinInfectious conditionsEpithelial responseFibronectin levelsEpithelial proliferationIntestinal epitheliumEpithelial cellsRepetitive forcesBasement membraneCollagen ICollagen IVIntracellular signalingP38JNK activationActivationCellsStrain activationTissueFibronectinRole of PP2A in the regulation of p38 MAPK activation in bovine aortic endothelial cells exposed to cyclic strain
Lee T, Kim SJ, Sumpio BE. Role of PP2A in the regulation of p38 MAPK activation in bovine aortic endothelial cells exposed to cyclic strain. Journal Of Cellular Physiology 2003, 194: 349-355. PMID: 12548554, DOI: 10.1002/jcp.10211.Peer-Reviewed Original ResearchConceptsP38 MAPK activationMAPK activationCatalytic subunitBovine aortic endothelial cellsPhospho-p38 MAPKOkadaic acidProtein phosphatase type 2AInhibitor of PP2AAortic endothelial cellsEndothelial cellsProtein kinaseRapid phosphorylationPP2AP38 mitogenRegulatory roleCell lysatesPhosphatase activityPP2AcMAPKPhosphorylationCyclic strainSubunitsType 2ACyclic strain resultsCells
2002
Cells in focus: endothelial cell
Sumpio BE, Riley JT, Dardik A. Cells in focus: endothelial cell. The International Journal Of Biochemistry & Cell Biology 2002, 34: 1508-1512. PMID: 12379270, DOI: 10.1016/s1357-2725(02)00075-4.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsMembrane-bound structuresEndothelial cellsPermeability barrierSelective permeability barrierExtracellular matrix componentsVon Willebrand factorWeibel-Palade bodiesStorage organellesGrowth regulationSplanchnopleuric mesodermMembrane functionMatrix componentsMultifunctional cellsBlood vessel toneCellsChemical stimuliWillebrand factorMesodermEndocrine organOrganellesCell injuryImmune responseVasculogenesisOrgansPathologic states
1998
Mechanical Strain Rapidly Redistributes Tyrosine Phosphorylated Proteins in Human Intestinal Caco-2 Cells
Han O, Sumpio B, Basson M. Mechanical Strain Rapidly Redistributes Tyrosine Phosphorylated Proteins in Human Intestinal Caco-2 Cells. Biochemical And Biophysical Research Communications 1998, 250: 668-673. PMID: 9784404, DOI: 10.1006/bbrc.1998.9372.Peer-Reviewed Original ResearchConceptsCaco-2 cellsTyrosine kinase activityHuman intestinal Caco-2 cellsIntestinal Caco-2 cellsCaco-2 intestinal cellsRepetitive strainHuman Caco-2 intestinal cellsCaco-2 intestinal epithelial cellsIntestinal epithelial cellsHuman Caco-2 intestinal epithelial cellsKinase activityTyrosine phosphorylationIntestinal cellsEpithelial cellsModulates differentiationParticulate fractionTyrosine phosphorylated proteinsCellsSubcellular distributionCoculture conditions alter endothelial modulation of TGF-β1 activation and smooth muscle growth morphology
Powell R, Bhargava J, Basson M, Sumpio B. Coculture conditions alter endothelial modulation of TGF-β1 activation and smooth muscle growth morphology. American Journal Of Physiology 1998, 274: h642-h649. PMID: 9486269, DOI: 10.1152/ajpheart.1998.274.2.h642.Peer-Reviewed Original ResearchCyclooxygenase Expression in Bovine Aortic Endothelial Cells Exposed to Cyclic Strain
Kito H, Yokoyama C, Inoue H, Tanabe T, Nakajima N, Sumpio B. Cyclooxygenase Expression in Bovine Aortic Endothelial Cells Exposed to Cyclic Strain. Endothelium 1998, 6: 107-112. PMID: 9930644, DOI: 10.3109/10623329809072197.Peer-Reviewed Original ResearchConceptsAortic endothelial cellsBovine aortic endothelial cellsEndothelial cellsInduction of COXCOX-1 expressionCOX-2 promoter activityExposure of ECsPromoter activityCycles/minCOX-2Cyclooxygenase expressionCOX-1Weak inducerMRNA expressionExtent of inductionNorthern blot analysisBlot analysisTransient transfection experimentsLipopolysaccharideMinimal effectExpressionInductionTransfection experimentsCyclic strainCells
1997
RAPID ALDOSTERONE‐INDUCED CELL VOLUME INCREASE OF ENDOTHELIAL CELLS MEASURED BY THE ATOMIC FORCE MICROSCOPE
SCHNEIDER S, YANO Y, SUMPIO B, JENA B, GEIBEL J, GEKLE M, OBERLEITHNER H. RAPID ALDOSTERONE‐INDUCED CELL VOLUME INCREASE OF ENDOTHELIAL CELLS MEASURED BY THE ATOMIC FORCE MICROSCOPE. Cell Biology International 1997, 21: 759-768. PMID: 9768474, DOI: 10.1006/cbir.1997.0220.Peer-Reviewed Original ResearchConceptsEndothelial cellsAddition of aldosteroneArterial blood pressureNon-genomic effectsCultured endothelial cellsBlood pressureMineralocorticoid hormonesHormone exposureAldosteroneCell volumeHuman leukocytesIntracellular electrolytesTransient swellingTriphosphate productionIntracellular pHLeukocytesVolume increaseBovine aortaContinuous presenceCellsPlasma membraneExposure of endothelial cells to cyclic strain induces elevations of cytosolic Ca2+ concentration through mobilization of intracellular and extracellular pools
ROSALES O, ISALES C, BARRETT P, BROPHY C, SUMPIO B. Exposure of endothelial cells to cyclic strain induces elevations of cytosolic Ca2+ concentration through mobilization of intracellular and extracellular pools. Biochemical Journal 1997, 326: 385-392. PMID: 9291109, PMCID: PMC1218682, DOI: 10.1042/bj3260385.Peer-Reviewed Original ResearchConceptsMicroM GdCl3Endothelial cellsIP3 formationLate-phase componentsInitiation of stretchExtracellular poolConfluent bovine aortic endothelial cellsMobilization of intracellularAortic endothelial cellsBovine aortic endothelial cellsNM charybdotoxinCycles/minIP3 generationIntracellular Ca2Sustained phaseCytosolic Ca2Confluent endothelial cellsBenzohydroquinoneFree mediumTemporal relationshipMicroM NiCl2Ca2PreincubationCellsInitial riseGene Regulation by Mechanical Forces
Oluwole BO, Du W, Mills I, Sumpio BE. Gene Regulation by Mechanical Forces. Endothelium 1997, 5: 85-93. PMID: 9237042, DOI: 10.3109/10623329709079866.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell Adhesion MoleculesCell Culture TechniquesCells, CulturedEndothelinsEndothelium, VascularEpoprostenolFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGene Expression RegulationHemorheologyHumansMechanoreceptorsMembranes, ArtificialNitric OxideProtein-Tyrosine KinasesSignal TransductionStress, MechanicalTissue Plasminogen ActivatorTranscription FactorsTranscription, GeneticVacuumConceptsEndothelial cellsEndothelial cell functionCyclic strainVasoactive mediatorsSecond messenger cascadesNitric oxideBlood vesselsMechanical forcesCell functionMechanical deformationFlow of bloodMessenger cascadesLuminal surfaceSpecific response elementsPromoter geneResponse elementExternal forcesMediatorsCytoskeletal changesCellsForceProstacyclinExpressionTranscription levels
1996
Protein phosphatase 2A in stretch‐induced endothelial cell proliferation
Murata K, Mills I, Sumpio B. Protein phosphatase 2A in stretch‐induced endothelial cell proliferation. Journal Of Cellular Biochemistry 1996, 63: 311-319. PMID: 8913882, DOI: 10.1002/(sici)1097-4644(19961201)63:3<311::aid-jcb6>3.0.co;2-#.Peer-Reviewed Original ResearchConceptsProtein phosphatase 2APhosphatase 2AProtein phosphatase 2A activityProtein phosphatase 1Phosphatase 2A activityProtein kinase CPhosphatase 1Bovine aortic endothelial cellsOkadaic acidGrowth suppressorEndothelial cellsKinase CEndothelial cell proliferationCell growthFetal bovine serumCellular proliferationAortic endothelial cellsCell proliferationCyclic strainBovine serumKey mechanismProliferationCellsStrainsSuppressor
1995
Cyclic strain upregulates nitric oxide synthase in cultured bovine aortic endothelial cells.
Awolesi MA, Sessa WC, Sumpio BE. Cyclic strain upregulates nitric oxide synthase in cultured bovine aortic endothelial cells. Journal Of Clinical Investigation 1995, 96: 1449-1454. PMID: 7544806, PMCID: PMC185768, DOI: 10.1172/jci118181.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseAortic endothelial cellsNitric oxide synthaseBovine aortic endothelial cellsCultured bovine aortic endothelial cellsEndothelial cellsOxide synthaseENOS transcriptsRegulation of eNOSENOS gene expressionBlot analysisHemodynamic forcesWestern blot analysisENOS levelsCycles/minImmunohistochemical stainingNitric oxideProtein levelsNorthern blot analysisNuclear runoff transcription assaysVivoCellsRunoff transcription assaysCytosolic RNAExpression
1994
Signal transduction pathways in vascular cells exposed to cyclic strain
Sumpio B, Du W, Cohen C, Evans L, Isales C, Rosales O, Mills I. Signal transduction pathways in vascular cells exposed to cyclic strain. 1994, 3-22. DOI: 10.1017/cbo9780511629068.001.Peer-Reviewed Original ResearchIncreased ambient pressure stimulates proliferation and morphologic changes in cultured endothelial cells
Sumpio B, Widmann M, Ricotta J, Awolesi M, Watase M. Increased ambient pressure stimulates proliferation and morphologic changes in cultured endothelial cells. Journal Of Cellular Physiology 1994, 158: 133-139. PMID: 8263020, DOI: 10.1002/jcp.1041580117.Peer-Reviewed Original ResearchConceptsSystemic blood pressureCultured endothelial cellsBlood pressureSystemic pressureCell responsesEndothelial cell responsesBovine aortic ECsMorphologic changesEndothelial cellsAortic ECsRat type IEC phenotypeSignificant increaseCell numberEC proliferationVascular systemSignificant regulatory influenceCalf serumEndotheliumType IDigital monitorRegulatory influenceProliferationHg pressureCells