2018
Regulation of Yes-Associated Protein by Laminar Flow
Chitragari G, Shalaby SY, Sumpio BJ, Kurita J, Sumpio BE. Regulation of Yes-Associated Protein by Laminar Flow. Annals Of Vascular Surgery 2018, 52: 183-191. PMID: 29758328, DOI: 10.1016/j.avsg.2018.03.002.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingCell ShapeCells, CulturedCytoskeletonDown-RegulationHuman Umbilical Vein Endothelial CellsHumansMechanotransduction, CellularPhosphoproteinsPhosphorylationProteolysisPulsatile FlowRegional Blood FlowStress, MechanicalTime FactorsTranscription FactorsYAP-Signaling ProteinsConceptsHuman umbilical vein endothelial cellsTotal YAPExposure of HUVECsRelease of mediatorsYAP levelsSignificant decreaseUbiquitin-proteasome system inhibitorsUmbilical vein endothelial cellsEndothelial cell proliferationVein endothelial cellsVascular healthSystem inhibitorsYes-Associated ProteinDisease modulationIntimal hyperplasiaDisturbed flowEndothelial cellsBlood vesselsDisease statesPotential targetCell proliferationProteasome-independent mechanismExposureKey regulatorPYAP
2012
Olive Oil Polyphenols Differentially Inhibit Smooth Muscle Cell Proliferation through a G1/S Cell Cycle Block Regulated by ERK1/2
Abe R, Beckett J, Abe R, Nixon A, Rochier A, Yamashita N, Sumpio B. Olive Oil Polyphenols Differentially Inhibit Smooth Muscle Cell Proliferation through a G1/S Cell Cycle Block Regulated by ERK1/2. International Journal Of Angiology 2012, 21: 069-076. PMID: 23730132, PMCID: PMC3444010, DOI: 10.1055/s-0032-1315630.Peer-Reviewed Original ResearchSmooth muscle cell proliferationMuscle cell proliferationCell cycle blockOlive oil polyphenolsCell cycle analysisDay 1SMC proliferationG1/S cell cycle blockCycle blockOil polyphenolsRisk of atherosclerosisCell proliferationOLE groupControl groupFlow cytometryVascular SMCsWestern blottingDays of exposureS phaseCycle analysisERK1/2 activationNumber of cellsCell populationsOlive oilG1 phase
2011
Olive Oil Polyphenol Oleuropein Inhibits Smooth Muscle Cell Proliferation
Abe R, Beckett J, Abe R, Nixon A, Rochier A, Yamashita N, Sumpio B. Olive Oil Polyphenol Oleuropein Inhibits Smooth Muscle Cell Proliferation. European Journal Of Vascular And Endovascular Surgery 2011, 41: 814-820. PMID: 21333557, DOI: 10.1016/j.ejvs.2010.12.021.Peer-Reviewed Original ResearchConceptsSmooth muscle cell proliferationMuscle cell proliferationCell cycle analysisSMC proliferationCell proliferationInhibits Smooth Muscle Cell ProliferationVascular smooth muscle cell proliferationCoronary artery diseaseNon-treated groupExtracellular signal-regulated kinase 1/2 activationCardiovascular mortalityArtery diseaseKinase 1/2 activationCell cycle blockG1-S phaseMediterranean dietDay 1Oleuropein treatmentGrowth of SMCsG1 phase regulatorsFlow cytometryVascular SMCsPresence of oleuropeinBeneficial effectsCycle analysis
2007
Effect of different frequencies of tensile strain on human dermal fibroblast proliferation and survival
Nishimura K, Blume P, Ohgi S, Sumpio BE. Effect of different frequencies of tensile strain on human dermal fibroblast proliferation and survival. Wound Repair And Regeneration 2007, 15: 646-656. PMID: 17971010, DOI: 10.1111/j.1524-475x.2007.00295.x.Peer-Reviewed Original ResearchConceptsMitogen-activated protein kinaseP38 mitogen-activated protein kinaseMAPK/ERK kinaseExtracellular signal-regulated kinaseDominant-negative AktHuman dermal fibroblastsSignal-regulated kinaseCell numberERK kinaseProtein kinaseTransduction pathwaysDermal fibroblastsRelevant transduction pathwaysRelevant signal pathwaysDermal fibroblast proliferationHuman dermal fibroblast proliferationCultured human dermal fibroblastsSurvival of fibroblastsSignal pathwayKinaseNecrotic fibroblastsDNA synthesisCell proliferationAktTransferase-mediated dUTP nick-end labeling stainingPhosphatase PTEN is inactivated in bovine aortic endothelial cells exposed to cyclic strain
Hoshino Y, Nishimura K, Sumpio BE. Phosphatase PTEN is inactivated in bovine aortic endothelial cells exposed to cyclic strain. Journal Of Cellular Biochemistry 2007, 100: 515-526. PMID: 16927376, DOI: 10.1002/jcb.21085.Peer-Reviewed Original ResearchConceptsCasein kinase 2Transfection of ECsPTEN plasmidVascular cell morphologyEndothelial cellsPI3K activitySuppression of apoptosisPI3K-Akt pathwayLipid phosphataseMaximal activityPhosphatase PTENPhospho-PTENPTEN activityBovine aortic endothelial cellsIntracellular phosphatidylinositolUpstream regulatorAkt activityKinase 2Phospho-AKT activityK activityTime-dependent mannerAortic endothelial cellsCell morphologyCell proliferationPTEN
2006
Role of AKT in cyclic strain-induced endothelial cell proliferation and survival
Nishimura K, Li W, Hoshino Y, Kadohama T, Asada H, Ohgi S, Sumpio BE. Role of AKT in cyclic strain-induced endothelial cell proliferation and survival. American Journal Of Physiology - Cell Physiology 2006, 290: c812-c821. PMID: 16469863, DOI: 10.1152/ajpcell.00347.2005.Peer-Reviewed Original ResearchConceptsEndothelial cellsCycles/minRole of AktEC proliferationCultured bovine aortic endothelial cellsSurvival of ECsAortic endothelial cellsApoptotic endothelial cellsGSK-3betaBovine aortic endothelial cellsEndothelial cell proliferationFrequency of strainsPositive cellsGlycogen synthase kinaseKinase-dead AktCell proliferationCaspase-3AktSurvivalCell numberSynthase kinaseProliferationEarly phosphorylationBad phosphorylation
2005
Resveratrol inhibits vascular smooth muscle cell proliferation and induces apoptosis
Poussier B, Cordova AC, Becquemin JP, Sumpio BE. Resveratrol inhibits vascular smooth muscle cell proliferation and induces apoptosis. Journal Of Vascular Surgery 2005, 42: 1190-1190.e14. PMID: 16376213, DOI: 10.1016/j.jvs.2005.08.014.Peer-Reviewed Original ResearchMeSH KeywordsAngiogenesis InhibitorsAnimalsAnimals, NewbornAntioxidantsAorta, ThoracicApoptosisBlotting, WesternCattleCell ProliferationCells, CulturedDNADose-Response Relationship, DrugFlow CytometryIn Situ Nick-End LabelingIn Vitro TechniquesMuscle, Smooth, VascularProliferating Cell Nuclear AntigenResveratrolStilbenesConceptsSmooth muscle cell proliferationMuscle cell proliferationVascular smooth muscle cell proliferationDose-dependent mannerSMC proliferationCell proliferationG1-S phaseBeneficial effectsFrench paradoxDose-dependent apoptotic effectTerminal deoxynucleotidyl transferase-mediated dUTP-biotin nickTransferase-mediated dUTP-biotin nickCardiovascular death ratesRed wine intakeRed wine consumptionEffects of resveratrolAortic SMC proliferationEnd labeling stainingVascular SMC proliferationDUTP-biotin nickMajor polyphenol componentHealthy life styleCell cycle analysisCardiovascular mortalityFlow-activated cell sortingDifferential responsiveness of early- and late-passage endothelial cells to shear stress
Kudo FA, Warycha B, Juran PJ, Asada H, Teso D, Aziz F, Frattini J, Sumpio BE, Nishibe T, Cha C, Dardik A. Differential responsiveness of early- and late-passage endothelial cells to shear stress. The American Journal Of Surgery 2005, 190: 763-769. PMID: 16226955, DOI: 10.1016/j.amjsurg.2005.07.017.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAortaApoptosisBlotting, WesternCattleCell CountCell DivisionCell ProliferationCells, CulturedEndothelium, VascularIn Vitro TechniquesMuscle, Smooth, VascularPhosphorylationProliferating Cell Nuclear AntigenProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktShear StrengthStress, MechanicalTumor Suppressor Protein p53ConceptsLate passage endothelial cellsOrbital shear stressEarly passage cellsSmooth muscle cell migrationMuscle cell migrationEndothelial cellsSenescence modelAkt phosphorylationCell migrationProtein kinase B activationPassage cellsKinase B activationCell proliferationVascular disease increasesLate passage cellsBovine aortic endothelial cellsNuclear antigen reactivityAortic endothelial cellsEndothelial cell proliferationNeointimal hyperplasiaAntigen reactivityTotal AktBoyden chamberB activationWestern blottingSustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway
Asada H, Paszkowiak J, Teso D, Alvi K, Thorisson A, Frattini JC, Kudo FA, Sumpio BE, Dardik A. Sustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway. Journal Of Vascular Surgery 2005, 42: 772-780. PMID: 16242567, DOI: 10.1016/j.jvs.2005.05.046.Peer-Reviewed Original ResearchConceptsOrbital shear stressPresence of PD98059Synthetic phenotypeERK1/2 pathwayExtracellular signal-regulated proteinSMC proliferationSmooth muscle cell proliferationMuscle cell proliferationPathway inhibitor PD98059Cell proliferationExtracellular signal-regulated protein kinase 1/2 (ERK1/2) phosphorylationKinase 1/2 phosphorylationPresence of SB203580Bovine SMCsSMC phenotypeInhibitor PD98059Inhibitor SB203580Contractile phenotypePD98059Long-term culturePhenotypeCell nuclear antigenExpression of markersPathwaySB203580
2004
Strain-induced vascular endothelial cell proliferation requires PI3K-dependent mTOR-4E-BP1 signal pathway
Li W, Sumpio BE. Strain-induced vascular endothelial cell proliferation requires PI3K-dependent mTOR-4E-BP1 signal pathway. AJP Heart And Circulatory Physiology 2004, 288: h1591-h1597. PMID: 15591103, DOI: 10.1152/ajpheart.00382.2004.Peer-Reviewed Original ResearchMeSH Keywords3-Phosphoinositide-Dependent Protein KinasesAndrostadienesAnimalsAntibiotics, AntineoplasticAortaCarrier ProteinsCattleCell DivisionCells, CulturedChromonesEndothelium, VascularEnzyme InhibitorsFlavonoidsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3MorpholinesPhosphatidylinositol 3-KinasesPhosphodiesterase InhibitorsPhosphoinositide-3 Kinase InhibitorsPhosphoproteinsPhosphorylationProtein KinasesProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktRibosomal Protein S6 KinasesSignal TransductionSirolimusTOR Serine-Threonine KinasesWortmanninConceptsVascular endothelial cell proliferationEndothelial cell proliferationStrain-induced activationSignal pathwayEC proliferationPD 98059Cell proliferationPI3K inhibitor wortmanninPI3K inhibitorsCycles/minExtracellular signal-regulated kinases 1Inhibitor PD 98059MTOR pathwaySignal-regulated kinases 1Bovine aortic ECsMammalian targetMTOR-4EK inhibitorsEukaryotic initiation factor 4EAortic ECsInitiation factor 4EMEK1 inhibitor PD 98059K activationProliferationRapamycinEffects of Cyclic Strain on Vascular Cells
Kakisis JD, Liapis CD, Sumpio BE. Effects of Cyclic Strain on Vascular Cells. Endothelium 2004, 11: 17-28. PMID: 15203876, DOI: 10.1080/10623320490432452.Peer-Reviewed Original ResearchConceptsCAMP-responsive elementVascular cell morphologyExtracellular mechanical signalsMitogen-activated protein kinaseProtein kinase CPlatelet-derived growth factor receptorEarly growth responseCells senseNumerous genesActivator proteinTranscription factorsProtein kinaseGrowth factor receptorAP-2Chemical signalsSecond messengerKinase CG proteinsMechanical signalsCell nucleiGrowth responseIon channelsCell morphologyFactor receptorCell proliferation
2003
Oscillatory shear stress increases smooth muscle cell proliferation and akt phosphorylation
Haga M, Yamashita A, Paszkowiak J, Sumpio BE, Dardik A. Oscillatory shear stress increases smooth muscle cell proliferation and akt phosphorylation. Journal Of Vascular Surgery 2003, 37: 1277-1284. PMID: 12764276, DOI: 10.1016/s0741-5214(03)00329-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell Physiological PhenomenaDisease Models, AnimalHemodynamicsIn Vitro TechniquesMyocytes, Smooth MuscleOscillometryPhosphatidylinositol 3-KinasesPhosphorylationProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktShear StrengthSignal TransductionStress, PhysiologicalVascular DiseasesConceptsSignal transduction pathwaysAkt phosphorylationTransduction pathwaysPI3K-Akt signal transduction pathwaySmooth muscle cell proliferationMuscle cell proliferationCell proliferationControl cellsOscillatory shear stressMaximal phosphorylationPI3K inhibitorsCell numberSurvival pathwaysAkt activationBovine aortic SMCSMC survivalInhibitor LY294002Akt pathwayPhosphorylationWestern blot techniqueControl survivalDNA synthesisK inhibitorsAortic SMCsPathway
2002
Inhibition of vascular smooth muscle cell proliferation with red wine and red wine polyphenols
Araim O, a J, Waterhouse AL, Sumpio BE. Inhibition of vascular smooth muscle cell proliferation with red wine and red wine polyphenols. Journal Of Vascular Surgery 2002, 35: 1226-1232. PMID: 12042735, DOI: 10.1067/mva.2002.124358.Peer-Reviewed Original ResearchConceptsRed wine polyphenol extractVascular SMC proliferationRed wine polyphenolsVascular smooth muscle cell proliferationSmooth muscle cell proliferationMuscle cell proliferationSMC proliferationAtherosclerotic diseaseBeneficial effectsWine polyphenolsVascular SMCsTrypan blue exclusion studiesDealcoholized red wineRed wine consumptionCell proliferationPolyphenol extractDose-dependent fashionInhibits SMC proliferationPotential beneficial effectsObserved beneficial effectsBovine aortic SMCDehydrogenase cytotoxicityAortic SMCsInhibitory effectRed wine
2000
Homocysteine stimulates MAP kinase in bovine aortic smooth muscle cells
Woo D, Dudrick S, Sumpio B. Homocysteine stimulates MAP kinase in bovine aortic smooth muscle cells. Surgery 2000, 128: 59-66. PMID: 10876187, DOI: 10.1067/msy.2000.106531.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell DivisionCells, CulturedDose-Response Relationship, DrugEnzyme ActivationEnzyme InhibitorsFlavonoidsHomocysteineMAP Kinase Signaling SystemMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesMuscle, Smooth, VascularPhosphorylationConceptsMAP kinase phosphorylationMAP kinaseKinase phosphorylationBASMC proliferationBovine aortic smooth muscle cellsAortic smooth muscle cellsCell proliferationExtracellular signal-regulated protein kinases 1Mitogen-activated protein kinaseSmooth muscle cell proliferationProtein kinase 1Smooth muscle cellsMuscle cell proliferationMAP kinase activationDegree of phosphorylationL-homocysteineMuscle cellsProtein kinaseKinase studiesKinase activationKinase 1Hallmark of atherosclerosisKinasePhosphorylationActive form
1999
Extracellular signal-regulated kinases 1 and 2 activation in endothelial cells exposed to cyclic strain
Ikeda M, Takei T, Mills I, Kito H, Sumpio B. Extracellular signal-regulated kinases 1 and 2 activation in endothelial cells exposed to cyclic strain. American Journal Of Physiology 1999, 276: h614-h622. PMID: 9950863, DOI: 10.1152/ajpheart.1999.276.2.h614.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCalciumCalcium-Calmodulin-Dependent Protein KinasesCattleCell DivisionCells, CulturedEndothelium, VascularEnzyme ActivationEnzyme InhibitorsExtracellular SpaceFlavonoidsIntracellular MembranesPhosphorylationProtein Kinase CProtein-Tyrosine KinasesStress, MechanicalConceptsERK1/ERK2Bovine aortic endothelial cellsExtracellular signal-regulated kinases 1ERK1/ERK2 activationExtracellular signal-regulated kinases 1/2Signal-regulated kinases 1/2Tyrosine kinase inhibitor genisteinCell proliferationTreatment of BAECProtein kinase C activationProtein kinase C inhibitor calphostin CTime-dependent phosphorylationKinase inhibitor genisteinKinase C activationERK2 activationEndothelial cellsERK2Strain-induced activationKinase 1Kinase 1/2PD 98059Tyrosine kinaseInhibitor genisteinCalphostin CStrain-dependent activation
1998
The differential effect of contrast agents on endothelial cell and smooth muscle cell growth in vitro
Sawmiller C, Powell R, Quader M, Dudrick S, Sumpio B. The differential effect of contrast agents on endothelial cell and smooth muscle cell growth in vitro. Journal Of Vascular Surgery 1998, 27: 1128-1140. PMID: 9652475, DOI: 10.1016/s0741-5214(98)70015-1.Peer-Reviewed Original ResearchConceptsNonionic contrastEndothelial cellsIonic contrastSmooth muscle cell proliferationSignificant decreaseSmooth muscle cell growthNonionic contrast agentsCultured bovine aorta endothelial cellsMuscle cell proliferationBrief exposureMuscle cell growthRole of osmolalityContrast exposureEC injuryContrast agentsAorta endothelial cellsLength of exposureDay 3SMC proliferationOsmolar effectsBovine aorta endothelial cellsEffect of contrastCell proliferationConfluent endothelial cellsOsmolar solution
1997
Strain activation of bovine aortic smooth muscle cell proliferation and alignment: Study of strain dependency and the role of protein kinase A and C signaling pathways
Mills I, Cohen C, Kamal K, Li G, Shin T, Du W, Sumpio B. Strain activation of bovine aortic smooth muscle cell proliferation and alignment: Study of strain dependency and the role of protein kinase A and C signaling pathways. Journal Of Cellular Physiology 1997, 170: 228-234. PMID: 9066778, DOI: 10.1002/(sici)1097-4652(199703)170:3<228::aid-jcp2>3.0.co;2-q.Peer-Reviewed Original ResearchMeSH KeywordsActivating Transcription Factor 2Adenylyl CyclasesAnimalsAortaCattleCell DivisionCells, CulturedCyclic AMPCyclic AMP Response Element-Binding ProteinCyclic AMP-Dependent Protein KinasesEndothelium, VascularLeucine ZippersMuscle, Smooth, VascularProtein Kinase CSignal TransductionStress, MechanicalTranscription FactorsConceptsBovine aortic SMCSMC proliferationCAMP response elementAortic SMCsAC/cAMP/PKAAortic smooth muscle cell proliferationSmooth muscle cell proliferationProtein levelsCyclic AMP accumulationMuscle cell proliferationSmooth muscle cell phenotypeExposure of SMCAdenylate cyclase activityMuscle cell phenotypeAMP accumulationInhibition of PKACAMP/PKACyclic AMP pathwayMultifactorial natureProtein kinase C activityCyclase activityProtein kinase A (PKA) activityLack of involvementEnhanced proliferationCell proliferation
1996
Protein phosphatase 2A in stretch‐induced endothelial cell proliferation
Murata K, Mills I, Sumpio B. Protein phosphatase 2A in stretch‐induced endothelial cell proliferation. Journal Of Cellular Biochemistry 1996, 63: 311-319. PMID: 8913882, DOI: 10.1002/(sici)1097-4644(19961201)63:3<311::aid-jcb6>3.0.co;2-#.Peer-Reviewed Original ResearchConceptsProtein phosphatase 2APhosphatase 2AProtein phosphatase 2A activityProtein phosphatase 1Phosphatase 2A activityProtein kinase CPhosphatase 1Bovine aortic endothelial cellsOkadaic acidGrowth suppressorEndothelial cellsKinase CEndothelial cell proliferationCell growthFetal bovine serumCellular proliferationAortic endothelial cellsCell proliferationCyclic strainBovine serumKey mechanismProliferationCellsStrainsSuppressor
1994
Inhibition of smooth muscle cell proliferation by visible light-activated psoralen.
Sumpio BE, Li G, Deckelbaum LI, Gasparro FP. Inhibition of smooth muscle cell proliferation by visible light-activated psoralen. Circulation Research 1994, 75: 208-213. PMID: 8033334, DOI: 10.1161/01.res.75.2.208.Peer-Reviewed Original Research
1993
Control of smooth muscle cell proliferation by psoralen photochemotherapy.
Sumpio B, Phan S, Gasparro F, Deckelbaum L. Control of smooth muscle cell proliferation by psoralen photochemotherapy. Journal Of Vascular Surgery 1993, 17: 1010-6; discussion 1016-8. PMID: 8505779, DOI: 10.1067/mva.1993.45747.Peer-Reviewed Original ResearchConceptsSmooth muscle cell proliferationMuscle cell proliferationSMC proliferationPsoralen photochemotherapyPeripheral vascular diseaseLong-term therapyCell proliferationInhibits SMC proliferationTrypan blue exclusionBypass graftDistal anastomosisBalloon angioplastyUVA photochemotherapyVascular diseaseIntimal hyperplasiaGrowth of SMCsBlue exclusionPhotochemotherapySMC viabilityTime courseProliferationUVADNA synthesisDaysAngioplasty