2003
Regulation of the intestinal epithelial response to cyclic strain by extracellular matrix proteins
Zhang J, Li W, Sanders MA, Sumpio BE, Asit P, Basson MD. Regulation of the intestinal epithelial response to cyclic strain by extracellular matrix proteins. The FASEB Journal 2003, 17: 1-22. PMID: 12626437, DOI: 10.1096/fj.02-0663fje.Peer-Reviewed Original ResearchMeSH KeywordsCaco-2 CellsCell AdhesionCell DivisionCell LineCollagen Type ICollagen Type IVEnzyme ActivationEpithelial CellsExtracellular Matrix ProteinsFibronectinsFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesHumansIntegrin alpha5Integrin alphaVIntestinal MucosaLamininMitogen-Activated Protein KinasesPhosphorylationProtein-Tyrosine KinasesStress, MechanicalConceptsExtracellular signal-regulated protein kinaseMatrix proteinsIntestinal epithelial responsesHuman intestinal epithelial proliferationSignal-regulated protein kinaseJun N-terminal kinaseFocal adhesion kinaseIntestinal epithelial biologyMatrix-dependent mannerN-terminal kinaseExtracellular matrix proteinsEpithelial responseCaco-2 proliferationCaco-2BBe cellsIntestinal epithelial proliferationAdhesion kinaseEpidermal growth factorProtein kinaseERK activationEpithelial biologyEpithelial cell proliferationIntegrin subunitsKinaseAnti-integrin antibodiesMEK blockade
2002
SPHINGOSINE-1-PHOSPHATE STIMULATES HUMAN CACO-2 INTESTINAL EPITHELIAL PROLIFERATION VIA p38 ACTIVATION AND ACTIVATES ERK BY AN INDEPENDENT MECHANISM
THAMILSELVAN V, LI W, SUMPIO BE, BASSON MD. SPHINGOSINE-1-PHOSPHATE STIMULATES HUMAN CACO-2 INTESTINAL EPITHELIAL PROLIFERATION VIA p38 ACTIVATION AND ACTIVATES ERK BY AN INDEPENDENT MECHANISM. In Vitro Cellular & Developmental Biology - Animal 2002, 38: 246-253. PMID: 12197778, DOI: 10.1290/1071-2690(2002)038<0246:spshci>2.0.co;2.Peer-Reviewed Original ResearchConceptsExtracellular signal-regulated kinases 1Mitogen-activated protein kinaseMAP kinase kinaseCaco-2 proliferationMAPK activationHuman intestinal epithelial proliferationP38 activationCell typesSignal-regulated kinases 1Role of ERKMitogenic effectCaco-2 intestinal epithelial cellsIntracellular second messengerMEK inhibitionP38 MAPK activationCancer cell invasionKinase kinaseHuman Caco-2 intestinal epithelial cellsProtein kinaseStimulation of proliferationCell motilityIntestinal epithelial cell proliferationInhibitor PD98059ERK2ERK activation
2001
Integrin and FAK-mediated MAPK activation is required for cyclic strain mitogenic effects in Caco-2 cells
Li W, Duzgun A, Sumpio B, Basson M. Integrin and FAK-mediated MAPK activation is required for cyclic strain mitogenic effects in Caco-2 cells. AJP Gastrointestinal And Liver Physiology 2001, 280: g75-g87. PMID: 11123200, DOI: 10.1152/ajpgi.2001.280.1.g75.Peer-Reviewed Original ResearchMeSH KeywordsCaco-2 CellsCell DivisionCell MovementCytoskeletal ProteinsEnzyme InhibitorsExtracellular MatrixFlavonoidsFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGene Expression Regulation, EnzymologicHumansImidazolesIntegrinsIntestinesMAP Kinase Signaling SystemMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein Kinase 8Mitogen-Activated Protein Kinase 9Mitogen-Activated Protein KinasesNaphthalenesP38 Mitogen-Activated Protein KinasesPaxillinPhosphoproteinsPhosphorylationProtein-Tyrosine KinasesPyridinesStress, MechanicalTransfectionConceptsFocal adhesion kinaseMAPK extracellular signal-regulated kinaseCaco-2 cellsMitogen-activated protein kinase activationVitro kinase assaysProtein kinase activationSignal-regulated kinaseMitogenic effectProtein kinase C inhibitionBeta1 integrin subunitsCaco-2 proliferationKinase assaysAdhesion kinaseFAK activationERK signalsJNK1 activationKinase activationTerminal kinaseMAPK activationUpstream signalsC-JunFAK inhibitionP38 inhibitionIntegrin subunitsKinase
2000
Effects of glutamine isomers on human (Caco-2) intestinal epithelial proliferation, strain-responsiveness, and differentiation
Murnin M, Kumar A, di Li G, Brown M, Sumpio B, Basson M. Effects of glutamine isomers on human (Caco-2) intestinal epithelial proliferation, strain-responsiveness, and differentiation. Journal Of Gastrointestinal Surgery 2000, 4: 435-442. PMID: 11058864, DOI: 10.1016/s1091-255x(00)80025-6.Peer-Reviewed Original ResearchMeSH KeywordsCaco-2 CellsCell CountCell DifferentiationCell DivisionDietary SupplementsDipeptidyl-Peptidases and Tripeptidyl-PeptidasesEnteral NutritionEnterocytesEnzyme InhibitorsEpithelial CellsFastingGamma-GlutamyltransferaseGastrointestinal MotilityGlutamineHumansIntestinal MucosaIsomerismIsoxazolesPeristalsisStatistics, NonparametricStress, MechanicalConceptsGlutamine supplementationIntestinal epithelial cellsBowel motilityGut mucosaCaco-2 proliferationEpithelial cellsCaco-2L-glutamine supplementationGlutamine metabolismCaco-2 intestinal epithelial cellsIntestinal epithelial proliferationFrequency-dependent mannerHuman Caco-2 intestinal epithelial cellsCaco-2 cellsEnteral feedingL-glutamineHT-29 cellsVillous motilitySelective antagonistEpithelial proliferationLess potencyVivo frequencyMalignant colonocytesHuman colonocytesMaximal stimulation