2017
Shear Stress Induces Change in Extracellular Signal-Regulated Kinase 5 Levels with Sustained Activation under Disturbed and Continuous Laminar Flow
Shalaby SY, Chitragari G, Sumpio BJ, Sumpio BE. Shear Stress Induces Change in Extracellular Signal-Regulated Kinase 5 Levels with Sustained Activation under Disturbed and Continuous Laminar Flow. International Journal Of Angiology 2017, 26: 109-115. PMID: 28566937, PMCID: PMC5446257, DOI: 10.1055/s-0037-1599057.Peer-Reviewed Original ResearchExtracellular signal-regulated kinase 5Pathogenesis of atherosclerosisExposure of HUVECERK5 activationVascular dysfunctionPattern of activationFro flowVascular remodelingConfluent HUVECsHours of exposurePhospho-ERK5Endothelial integrityActivation of ERK5Kinase 5Sustained activationEnd of experimentationERK5 phosphorylationHUVECActivationHoursExposureTransient phosphorylationCell lysatesDisturbed flow conditionsParallel plate flow chamber
2007
Role of Ligand-Specific Integrins in Endothelial Cell Alignment and Elongation Induced by Cyclic Strain
Hirayama Y, Sumpio BE. Role of Ligand-Specific Integrins in Endothelial Cell Alignment and Elongation Induced by Cyclic Strain. Endothelium 2007, 14: 275-283. PMID: 18080865, DOI: 10.1080/10623320701746248.Peer-Reviewed Original ResearchConceptsEndothelial cellsP38 MAPKAnti-alpha2 integrin antibodyMorphological changesP38 mitogen-activated protein kinase (MAPK) pathwayCycles/minIntegrin-blocking antibodiesP38 MAPK pathwayMitogen-activated protein kinase pathwayAlpha 5Integrin antibodyProtein kinase pathwayMAPK pathwayAntibodiesKinase pathwayMAPKMultiple biological processesActivationIntegrinsCritical roleCyclic strainPathway
2006
p38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress
Kadohama T, Akasaka N, Nishimura K, Hoshino Y, Sasajima T, Sumpio BE. p38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress. Endothelium 2006, 13: 43-50. PMID: 16885066, DOI: 10.1080/10623320600660219.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesAtherosclerosisBlood PressureBlood VesselsCattleCell PolarityCell ShapeCells, CulturedDose-Response Relationship, DrugEndothelial CellsEnzyme ActivationEnzyme InhibitorsHemodynamicsMAP Kinase Signaling SystemP38 Mitogen-Activated Protein KinasesPhosphorylationRegional Blood FlowStress, MechanicalTime FactorsConceptsStatic endothelial cellsFluid shear stressP38 MAPKP38 Mitogen-Activated Protein Kinase ActivationMitogen-Activated Protein Kinase ActivationEndothelial cellsProtein kinase activationP38 MAPK inhibitor SB-203580Inhibitor SB 203580Cell alignmentProtein kinaseBovine aortic endothelial cellsLaminar shear stressKinase activationSB 203580P38 mitogenCultured bovine aortic endothelial cellsEC elongationAortic endothelial cellsMAPKCellsElongationActivationMorphometric analysisKinase
2003
Fibronectin blocks p38 and jnk activation by cyclic strain in Caco-2 cells
Zhang J, Li W, Sumpio BE, Basson MD. Fibronectin blocks p38 and jnk activation by cyclic strain in Caco-2 cells. Biochemical And Biophysical Research Communications 2003, 306: 746-749. PMID: 12810082, DOI: 10.1016/s0006-291x(03)01044-1.Peer-Reviewed Original ResearchConceptsIntestinal epithelial responsesIntestinal epithelial proliferationCaco-2 intestinal epithelial cellsIntestinal epithelial cellsHuman Caco-2 intestinal epithelial cellsCaco-2 cellsPlasma fibronectinInfectious conditionsEpithelial responseFibronectin levelsEpithelial proliferationIntestinal epitheliumEpithelial cellsRepetitive forcesBasement membraneCollagen ICollagen IVIntracellular signalingP38JNK activationActivationCellsStrain activationTissueFibronectin
1999
Phosphatidylinositol-3 Kinase Dependent MAP Kinase Activation via p21ras in Endothelial Cells Exposed to Cyclic Strain
Ikeda M, Kito H, Sumpio B. Phosphatidylinositol-3 Kinase Dependent MAP Kinase Activation via p21ras in Endothelial Cells Exposed to Cyclic Strain. Biochemical And Biophysical Research Communications 1999, 257: 668-671. PMID: 10208841, DOI: 10.1006/bbrc.1999.0532.Peer-Reviewed Original ResearchMeSH KeywordsAndrostadienesAnimalsAortaCalcium-Calmodulin-Dependent Protein KinasesCattleCells, CulturedChromonesEndothelium, VascularEnzyme ActivationGuanosine TriphosphateHemodynamicsMorpholinesOncogene Protein p21(ras)Phosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphorylationSignal TransductionStress, MechanicalTime FactorsWortmanninConceptsERK1/2 activationPhosphatidylinositol-3 kinase inhibitorMAP kinase activationExtracellular signal-regulated protein kinases 1Endothelial cellsMechanical stretchKinase inhibitorsERK1/2 phosphorylationEffect of wortmanninPossible involvementHemodynamic forcesProtein kinase 1ActivationKinase 1Upstream pathwaysRapid activationKinase activationSignal transduction pathwaysP21ras activationLY294002ERK1 activationERK2 activation
1998
Calcium-Independent Activation of Extracellular Signal-Regulated Kinases 1 and 2 by Cyclic Strain
Ikeda M, Takei T, Mills I, Sumpio B. Calcium-Independent Activation of Extracellular Signal-Regulated Kinases 1 and 2 by Cyclic Strain. Biochemical And Biophysical Research Communications 1998, 247: 462-465. PMID: 9642151, DOI: 10.1006/bbrc.1998.8811.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium Channel BlockersCalcium-Calmodulin-Dependent Protein KinasesCattleCells, CulturedChelating AgentsEgtazic AcidEndothelium, VascularEnzyme ActivationExtracellular SpaceGadoliniumHydroquinonesIntracellular FluidMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesPhosphorylationStress, MechanicalConceptsEndothelial cellsActivation of ERK1/2Baseline phosphorylationExtracellular Ca2Normal extracellular Ca2Aortic endothelial cellsKinase 1Bovine aortic endothelial cellsStrain-induced activationCycles/minChannel blockersExtracellular signal-regulated kinases 1Signal-regulated kinases 1Calcium-independent activationBenzohydroquinoneERK1/2 activationERK1/2Free mediumEffect of Ca2ActivationCa2EGTACoculture conditions alter endothelial modulation of TGF-β1 activation and smooth muscle growth morphology
Powell R, Bhargava J, Basson M, Sumpio B. Coculture conditions alter endothelial modulation of TGF-β1 activation and smooth muscle growth morphology. American Journal Of Physiology 1998, 274: h642-h649. PMID: 9486269, DOI: 10.1152/ajpheart.1998.274.2.h642.Peer-Reviewed Original Research
1993
Endothelin-Stimulated Human Monocytes Produce Prostaglandin E2 but Not Leukotriene B4
McMillen M, Huribal M, Kumar R, Sumpio B. Endothelin-Stimulated Human Monocytes Produce Prostaglandin E2 but Not Leukotriene B4. Journal Of Surgical Research 1993, 54: 331-335. PMID: 8392650, DOI: 10.1006/jsre.1993.1053.Peer-Reviewed Original ResearchConceptsProstaglandin E2Pg/Human monocytesPost-traumatic immunosuppressionMonocytes/macrophagesBacterial lipopolysaccharide (LPS) stimulationEndothelin increaseM endothelinInterleukin-6Immune functionLeukotriene B4PGE2 productionVascular endotheliumEndothelinLipopolysaccharide (LPS) stimulationInjuryMonocytesAcid peptideE2B4HrActivationImmunosuppressionIschemiaMajor role