2024
Molecular Imaging of Collagen Turnover in Myocardial Infarction
Neishabouri A, Ghim M, Ahmad A, Kukreja G, Zhang Z, Li J, Salarian M, Toczek J, Gona K, Hedayatyanfard K, Zhang J, Huang H, Yu S, Sadeghi M. Molecular Imaging of Collagen Turnover in Myocardial Infarction. Journal Of Nuclear Cardiology 2024, 38: 101998. DOI: 10.1016/j.nuclcard.2024.101998.Peer-Reviewed Original Research
2023
Multimodality Imaging of Aortic Valve Calcification and Function in a Murine Model of Calcific Aortic Valve Disease and Bicuspid Aortic Valve
Ahmad A, Ghim M, Toczek J, Neishabouri A, Ojha D, Zhang Z, Gona K, Raza M, Jung J, Kukreja G, Zhang J, Guerrera N, Liu C, Sadeghi M. Multimodality Imaging of Aortic Valve Calcification and Function in a Murine Model of Calcific Aortic Valve Disease and Bicuspid Aortic Valve. Journal Of Nuclear Medicine 2023, 64: 1487-1494. PMID: 37321825, PMCID: PMC10478817, DOI: 10.2967/jnumed.123.265516.Peer-Reviewed Original ResearchConceptsF-NaF PET/CTCalcific aortic valve diseaseBicuspid aortic valvePET/CTAortic valve calcificationAortic valve diseaseAortic valveAortic stenosisValve calcificationValvular calcificationValve diseaseF-NaFSubset of miceTricuspid aortic valveDevelopment of calcificationSignificant correlationUnderwent echocardiographyMedical therapyHigh prevalencePreclinical modelsMurine modelTherapeutic interventionsAge groupsAutoradiography dataMultimodality imagingPositron Emission Tomography Imaging of Vessel Wall Matrix Metalloproteinase Activity in Abdominal Aortic Aneurysm
Toczek J, Gona K, Liu Y, Ahmad A, Ghim M, Ojha D, Kukreja G, Salarian M, Luehmann H, Heo G, Guzman R, Chaar C, Tellides G, Hassab A, Ye Y, Shoghi K, Zayed M, Gropler R, Sadeghi M. Positron Emission Tomography Imaging of Vessel Wall Matrix Metalloproteinase Activity in Abdominal Aortic Aneurysm. Circulation Cardiovascular Imaging 2023, 16: e014615. PMID: 36649454, PMCID: PMC9858355, DOI: 10.1161/circimaging.122.014615.Peer-Reviewed Original ResearchConceptsAbdominal aortic aneurysmHuman aortic tissueMMP activityAortic tissueAortic aneurysmMMP inhibitorsMurine modelMatrix metalloproteinasesPositron emission tomography/Toxicology studiesAortic MMP activityPositron emission tomography radioligandAngiotensin II infusionEmission tomography/Murine abdominal aortic aneurysmsBroad-spectrum MMP inhibitorMatrix metalloproteinase activityPositron emission tomography (PET) tracerPositron emission tomography (PET) imagingEmission Tomography ImagingPositron emission tomographyEmission tomography tracerII infusionRisk stratificationTomography/
2022
TRPC1 channels underlie stretch-modulated sarcoplasmic reticulum calcium leak in cardiomyocytes
Streiff M, Corbin A, Ahmad A, Hunter C, Sachse F. TRPC1 channels underlie stretch-modulated sarcoplasmic reticulum calcium leak in cardiomyocytes. Frontiers In Physiology 2022, 13: 1056657. PMID: 36620209, PMCID: PMC9817106, DOI: 10.3389/fphys.2022.1056657.Peer-Reviewed Original ResearchNeonatal rat ventricular myocytesTransient receptor potentialTRPC1 channelsTRPC1 expressionCardiac diseaseReceptor potentialSarcoplasmic reticulum calcium leakAdenoviral transfectionRat ventricular myocytesSarco/endoplasmic reticulum CaPermeable ion channelsPhysiological roleSR leakPharmacological therapyEndoplasmic reticulum CaCalcium leakMechanosensitive modulationVentricular myocytesWestern blotReticulum CaRT-qPCRTRPC1CardiomyocytesDisease developmentSignificant reductionDifferential BMP Signaling Mediates the Interplay Between Genetics and Leaflet Numbers in Aortic Valve Calcification
Jung JJ, Ahmad AA, Rajendran S, Wei L, Zhang J, Toczek J, Nie L, Kukreja G, Salarian M, Gona K, Ghim M, Chakraborty R, Martin KA, Tellides G, Heistad D, Sadeghi MM. Differential BMP Signaling Mediates the Interplay Between Genetics and Leaflet Numbers in Aortic Valve Calcification. JACC Basic To Translational Science 2022, 7: 333-345. PMID: 35540096, PMCID: PMC9079798, DOI: 10.1016/j.jacbts.2021.12.006.Peer-Reviewed Original ResearchBicuspid aortic valveCalcific aortic valve diseaseAortic valve calcificationValve calcificationHuman Calcific Aortic Valve DiseaseAortic valve diseaseValve diseaseAortic valveCAVD progressionTherapeutic targetBone morphogenic proteinMorphogenic proteinMiceCalcificationDCBLD2StenosisExpressionLittermatesDiseaseBMP signalingProgression
2020
Modulation of Calcium Transients in Cardiomyocytes by Transient Receptor Potential Canonical 6 Channels
Ahmad A, Streiff M, Hunter C, Sachse F. Modulation of Calcium Transients in Cardiomyocytes by Transient Receptor Potential Canonical 6 Channels. Frontiers In Physiology 2020, 11: 44. PMID: 32116757, PMCID: PMC7033547, DOI: 10.3389/fphys.2020.00044.Peer-Reviewed Original ResearchNeonatal rat ventricular myocytesTRPC6 channelsCytosolic calcium concentrationCalcium loadTransient Receptor Potential Canonical 6 ChannelsCalcium transientsCalcium concentrationSarcoplasmic reticulum calcium loadCardiac hypertrophic remodelingActivated T cellsCalcineurin-nuclear factorEnhanced-green fluorescent proteinRat ventricular myocytesNon-selective cation channelsTransient receptor potentialCytosolic calcium transientsHypertrophic remodelingSustained elevationSR calcium loadT cellsIntracellular calciumCardiac hypertrophyCalcium dyeIndo-1Ventricular myocytesLocation and function of transient receptor potential canonical channel 1 in ventricular myocytes
Hu Q, Ahmad A, Seidel T, Hunter C, Streiff M, Nikolova L, Spitzer K, Sachse F. Location and function of transient receptor potential canonical channel 1 in ventricular myocytes. Journal Of Molecular And Cellular Cardiology 2020, 139: 113-123. PMID: 31982426, PMCID: PMC7085981, DOI: 10.1016/j.yjmcc.2020.01.008.Peer-Reviewed Original ResearchConceptsNeonatal rat ventricular myocytesRabbit ventricular myocytesVentricular myocytesSarcoplasmic reticulumChannel blocker SKF-96365TRPC1 overexpressionTransient receptor potential canonical channel 1Physiological pacing ratesTRPC1 channelsAdult rabbit ventricular myocytesRole of TRPC1Rat ventricular myocytesSarco/endoplasmic reticulum CaTransient receptor potentialMammalian ventricular myocytesIsolated rabbit cardiomyocytesEndoplasmic reticulum CaSKF-96365Free solutionCardiac hypertrophyPacing rateAction potentialsTRPC channelsTransverse tubular systemReticulum Ca
2017
Physiological and pathophysiological role of transient receptor potential canonical channels in cardiac myocytes
Ahmad A, Streiff M, Hunter C, Hu Q, Sachse F. Physiological and pathophysiological role of transient receptor potential canonical channels in cardiac myocytes. Progress In Biophysics And Molecular Biology 2017, 130: 254-263. PMID: 28629808, DOI: 10.1016/j.pbiomolbio.2017.06.005.Peer-Reviewed Original ResearchConceptsTransient receptor potential canonical (TRPC) channelsTRPC channelsCanonical channelsHypertrophic signalingCardiac pathophysiologyPhysiological rolePermeable ion channelsNon-cardiac cellsPathophysiological roleCardiac diseaseHeart rateTopic of controversyTRPC activityPharmacological toolsSuggested physiological rolesCardiac physiologyCardiac myocytesMammalian heartCardiac cellsPathophysiologyPrecise roleTRPC1HeartIon channelsCell typesSheet-Like Remodeling of the Transverse Tubular System in Human Heart Failure Impairs Excitation-Contraction Coupling and Functional Recovery by Mechanical Unloading
Seidel T, Navankasattusas S, Ahmad A, Diakos N, Xu W, Tristani-Firouzi M, Bonios M, Taleb I, Li D, Selzman C, Drakos S, Sachse F. Sheet-Like Remodeling of the Transverse Tubular System in Human Heart Failure Impairs Excitation-Contraction Coupling and Functional Recovery by Mechanical Unloading. Circulation 2017, 135: 1632-1645. PMID: 28073805, PMCID: PMC5404964, DOI: 10.1161/circulationaha.116.024470.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedBiopsyCase-Control StudiesEchocardiographyExcitation Contraction CouplingFemaleHeart FailureHeart-Assist DevicesHumansImage Processing, Computer-AssistedMaleMicroscopy, ConfocalMiddle AgedMyocardial ContractionMyocytes, CardiacProspective StudiesProsthesis DesignRecovery of FunctionRyanodine Receptor Calcium Release ChannelTime FactorsTreatment OutcomeVentricular Function, LeftVentricular RemodelingConceptsLeft ventricular assist deviceChronic heart failureHeart failureExcitation-contraction couplingCardiac recoveryMechanical unloadingLVAD implantationEjection fractionFunctional recoveryHF myocytesImplantation of LVADHallmark of HFHuman end-stage heart failureLeft ventricular ejection fractionEnd-stage heart failureTransverse tubular systemEnd-stage human heart failureEjection fraction increaseIsolated human cardiomyocytesVentricular ejection fractionSubgroup of patientsVolume/length ratioHuman heart failureFunctional cardiac recoveryVentricular assist device