2019
Mutagenesis of the ADAM17-phosphatidylserine–binding motif leads to embryonic lethality in mice
Veit M, Ahrens B, Seidel J, Sommer A, Bhakdi S, Reiss K. Mutagenesis of the ADAM17-phosphatidylserine–binding motif leads to embryonic lethality in mice. Life Science Alliance 2019, 2: e201900430. PMID: 31455669, PMCID: PMC6712283, DOI: 10.26508/lsa.201900430.Peer-Reviewed Original ResearchConceptsEmbryonic lethalityVital cellular functionsAnionic phospholipid headgroupsTransmembrane substratesCellular functionsProtease functionBinding motifAmino acidsCell surfaceMutant proteasePrimary hepatocytesMotifMutagenesisPhosphatidylserinePhospholipid headgroupsProminent memberCentral roleEssential triggerLethalitySurface exposureOncogenesisProteaseADAM17DisintegrinMice
2018
Anoctamin-6 regulates ADAM sheddase function
Veit M, Koyro K, Ahrens B, Bleibaum F, Munz M, Rövekamp H, Andrä J, Schreiber R, Kunzelmann K, Sommer A, Bhakdi S, Reiss K. Anoctamin-6 regulates ADAM sheddase function. Biochimica Et Biophysica Acta (BBA) - Molecular Cell Research 2018, 1865: 1598-1610. PMID: 30327201, DOI: 10.1016/j.bbamcr.2018.08.011.Peer-Reviewed Original ResearchConceptsAnoctamin 6Scramblase activityHuman umbilical vein endothelial cellsTNF receptor 1Vital cellular functionsPhospholipid scramblase activityPS exposureHEK293T cellsTGF-alphaTransformation of cellsSheddase functionTransmembrane substratesCellular functionsADAM17 substratesPlasma membraneUmbilical vein endothelial cellsInhibitor experimentsSheddase activitySubstrate cleavageImportant regulatorVein endothelial cellsActivator PMAProminent memberAmphiregulinEndothelial cells
2015
Extracellular Juxtamembrane Segment of ADAM17 Interacts with Membranes and Is Essential for Its Shedding Activity
Düsterhöft S, Michalek M, Kordowski F, Oldefest M, Sommer A, Röseler J, Reiss K, Grötzinger J, Lorenzen I. Extracellular Juxtamembrane Segment of ADAM17 Interacts with Membranes and Is Essential for Its Shedding Activity. Biochemistry 2015, 54: 5791-5801. PMID: 26348730, DOI: 10.1021/acs.biochem.5b00497.Peer-Reviewed Original ResearchConceptsMembrane-proximal domainJuxtamembrane segmentSubstrate recognitionMembrane-anchored proteinsAmino acid residuesActivity of ADAM17Extracellular regionBiological processesMajor sheddasesAcid residuesCancer progressionLipid bilayersMetalloprotease 17Key mediatorInterleukin-6 receptorDisintegrinADAM17SheddasesDomainProteinInteractsResiduesDifferentiationActivityWide variety