2021
Suppression of Kv3.3 channels by antisense oligonucleotides reverses biochemical effects and motor impairment in spinocerebellar ataxia type 13 mice
Zhang Y, Quraishi IH, McClure H, Williams LA, Cheng Y, Kale S, Dempsey GT, Agrawal S, Gerber DJ, McManus OB, Kaczmarek LK. Suppression of Kv3.3 channels by antisense oligonucleotides reverses biochemical effects and motor impairment in spinocerebellar ataxia type 13 mice. The FASEB Journal 2021, 35: e22053. PMID: 34820911, PMCID: PMC8630780, DOI: 10.1096/fj.202101356r.Peer-Reviewed Original ResearchConceptsHAX-1Wild-type animalsMultivesicular bodiesKv3.3 channelsLate endosomes/multivesicular bodiesTank Binding Kinase 1Type animalsCell survival proteinsDisease-causing mutationsVoltage-dependent potassium channelsSpinocerebellar ataxia type 13Survival proteinsKinase 1Mature intact animalsTBK1 activationAge-matched wild-type animalsLevels of CD63Progressive cerebellar degenerationWild-type miceMutationsProtein levelsMutant micePotassium channelsDependent potassium channelsType mice
2017
Tuning Neuronal Potassium Channels to the Auditory Environment
Kaczmarek L. Tuning Neuronal Potassium Channels to the Auditory Environment. Springer Handbook Of Auditory Research 2017, 64: 133-159. DOI: 10.1007/978-3-319-21530-3_6.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsBrainstem nucleiPotassium channelsAuditory brainstem nucleiVoltage-dependent potassium channelsNeuronal potassium channelsAuditory discrimination taskAuditory neuronsAuditory environmentChannel isoformsNeuronsHigh rateAuditory informationKv3.1Molecular mechanismsDifferent auditory environmentsRapid alterationsDiscrimination task
2015
Kv3.3 potassium channels and spinocerebellar ataxia
Zhang Y, Kaczmarek LK. Kv3.3 potassium channels and spinocerebellar ataxia. The Journal Of Physiology 2015, 594: 4677-4684. PMID: 26442672, PMCID: PMC4983625, DOI: 10.1113/jp271343.Peer-Reviewed Original ResearchConceptsPurkinje cellsPotassium channelsAuditory brainstem nucleiCentral nervous systemUnique neurodegenerative diseaseCerebellar Purkinje cellsVoltage-dependent potassium channelsSpinocerebellar ataxia type 13Neuronal survivalBrainstem nucleiExtracerebellar symptomsCerebellar degenerationNervous systemNeurodegenerative diseasesComplex spikesNormal functionKv3.3Disease-causing mutationsType 13Kv3.3 potassium channelSpinocerebellar ataxiaHigh rateCerebellumDifferent mutationsPhysiological functions
2010
The Slack Sodium-Activated Potassium Channel Provides a Major Outward Current in Olfactory Neurons of Kv1.3−/− Super-Smeller Mice
Lu S, Das P, Fadool DA, Kaczmarek LK. The Slack Sodium-Activated Potassium Channel Provides a Major Outward Current in Olfactory Neurons of Kv1.3−/− Super-Smeller Mice. Journal Of Neurophysiology 2010, 103: 3311-3319. PMID: 20393063, PMCID: PMC2888249, DOI: 10.1152/jn.00607.2009.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBiophysicsCardiovascular AgentsCells, CulturedElectric StimulationGene Expression RegulationIn Vitro TechniquesKv1.3 Potassium ChannelMembrane PotentialsMiceMice, Inbred C57BLMice, KnockoutNerve Tissue ProteinsNeuronsOlfactory BulbPatch-Clamp TechniquesPotassium ChannelsPotassium Channels, Sodium-ActivatedPyrimidinesRNA InterferenceSodium Channel BlockersTetrodotoxinTransfectionConceptsMitral cellsOlfactory bulbOutward currentsOlfactory neuronsWildtype animalsPotassium channelsMajor outward currentVoltage-clamp recordingsVoltage-dependent potassium channelsNet outward currentIntracellular sodiumOB slicesPotassium channel genesCompensatory increaseFiring patternsWestern blottingRNA interference approachPrimary culturesEnhanced expressionDetection of odorsSame treatmentChannel genesMiceNeuronsOlfactory phenotypes
2005
Regulation of the timing of MNTB neurons by short-term and long-term modulation of potassium channels
Kaczmarek LK, Bhattacharjee A, Desai R, Gan L, Song P, von Hehn CA, Whim MD, Yang B. Regulation of the timing of MNTB neurons by short-term and long-term modulation of potassium channels. Hearing Research 2005, 206: 133-145. PMID: 16081004, DOI: 10.1016/j.heares.2004.11.023.Peer-Reviewed Original ResearchConceptsAnteroventral cochlear nucleusPotassium channelsAuditory pathwayAction potentialsCentral auditory pathwayVoltage-dependent potassium channelsMammalian auditory pathwayAmount of neurotransmitterProtein phosphorylationMNTB neuronsGene expressionBushy cellsPrincipal neuronsTrapezoid bodyCochlear nucleusIntrinsic excitabilityMedial nucleusVoltage-dependent channelsFiring patternsNeuronsAmplitude of currentsKv1 familySound stimuliLong-term modulationSound localization
1992
Expression of the H-ras oncogene induces potassium conductance and neuron-specific potassium channel mRNAs in the AtT20 cell line
Hemmick L, Perney T, Flamm R, Kaczmarek L, Birnberg N. Expression of the H-ras oncogene induces potassium conductance and neuron-specific potassium channel mRNAs in the AtT20 cell line. Journal Of Neuroscience 1992, 12: 2007-2014. PMID: 1607925, PMCID: PMC6575920, DOI: 10.1523/jneurosci.12-06-02007.1992.Peer-Reviewed Original ResearchConceptsRas-transfected cellsAtT20 cell lineAtT20 cellsEJ-ras oncogeneCell linesPotassium channelsVoltage-dependent potassium channelsRas proteinsPituitary-derived cellsMRNA speciesPlasma membraneSpliced productsPotassium channel mRNASame geneChannel mRNADifferential expressionPotassium currentExcitable cellsVoltage-dependent potassium currentsTwo- to threefold increaseMRNAOncogeneSpeciesExpressionPhenotype