2021
Knockdown of Claudin-19 in the Retinal Pigment Epithelium Is Accompanied by Slowed Phagocytosis and Increased Expression of SQSTM1
Liu F, Peng S, Adelman RA, Rizzolo LJ. Knockdown of Claudin-19 in the Retinal Pigment Epithelium Is Accompanied by Slowed Phagocytosis and Increased Expression of SQSTM1. Investigative Ophthalmology & Visual Science 2021, 62: 14-14. PMID: 33591357, PMCID: PMC7900869, DOI: 10.1167/iovs.62.2.14.Peer-Reviewed Original ResearchConceptsSequestosome 1/p62Photoreceptor outer segmentsInternalized photoreceptor outer segmentsOxidative stress responseApical junctional complexActivation of AMPKTransepithelial electrical resistanceFluorescence-activated cell sortingWestern blottingClaudin-19Expression of proteinsSteady-state levelsProtein kinaseFilamentous actinEffect of AMPStress responseQuantitative RT-PCRKnockdownPOS degradationAMPKJunctional complexesEnzymatic activityCell sortingExpression of metallothioneinProteasome inhibitors
2020
Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium
Singh D, Chen X, Xia T, Ghiassi-Nejad M, Tainsh L, Adelman RA, Rizzolo LJ. Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium. Investigative Ophthalmology & Visual Science 2020, 61: 9-9. PMID: 33151282, PMCID: PMC7671856, DOI: 10.1167/iovs.61.13.9.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumRetinal progenitor cellsTransepithelial electrical resistanceNeurosensory retinaPigment epitheliumHuman fetal retinal pigment epitheliumRecoverin-positive cellsFetal retinal pigment epitheliumGanglion cell layerAbility of RPERPE signature genesRed/green opsinTranswell filter insertsStem cellsQuantitative RT-PCRGlial markersHuman stem cellsExpression of rhodopsinInterneuron markersBipolar cellsRetinal culturesRetinaRT-PCRProgenitor cellsPresumptive photoreceptors
2017
A biodegradable scaffold enhances differentiation of embryonic stem cells into a thick sheet of retinal cells
Singh D, Wang SB, Xia T, Tainsh L, Ghiassi-Nejad M, Xu T, Peng S, Adelman RA, Rizzolo LJ. A biodegradable scaffold enhances differentiation of embryonic stem cells into a thick sheet of retinal cells. Biomaterials 2017, 154: 158-168. PMID: 29128844, DOI: 10.1016/j.biomaterials.2017.10.052.Peer-Reviewed Original ResearchConceptsRetinal degenerationNeurosensory retinaRetinal cellsHost retinal pigment epitheliumStem cellsOuter nuclear layerRetinal pigment epitheliumRetinal cell typesElaborate arborsQuantitative RT-PCRLeading causeImmune responseNuclear layerMouse modelPigment epitheliumSubretinal spaceForebrain cellsMinimal immune responseTherapeutic agentsRetinal laminationRT-PCRDegenerationRetinaRetinal organoidsConfocal immunocytochemistry
2015
TRP Channels Localize to Subdomains of the Apical Plasma Membrane in Human Fetal Retinal Pigment EpitheliumTRP Channels of Human Fetal RPE
Zhao PY, Gan G, Peng S, Wang SB, Chen B, Adelman RA, Rizzolo LJ. TRP Channels Localize to Subdomains of the Apical Plasma Membrane in Human Fetal Retinal Pigment EpitheliumTRP Channels of Human Fetal RPE. Investigative Ophthalmology & Visual Science 2015, 56: 1916-1923. PMID: 25736794, PMCID: PMC4364639, DOI: 10.1167/iovs.14-15738.Peer-Reviewed Original ResearchConceptsHuman fetal RPETRP channelsApical membraneFetal RPEApical plasma membraneCell-cell contactTransepithelial electrical resistanceTransient receptor potential channelsTight junctionsSubcellular localizationInhibitor of calpainPrimary ciliaRT-PCRPlasma membraneBasal channel activityQuantitative RT-PCRApical tight junctionsExpression of TRPC4Ion channelsBasolateral surfaceApical microvilliApical surfaceChannel activityConfocal microscopyTRPM3
2011
Claudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium
Peng S, Rao VS, Adelman RA, Rizzolo LJ. Claudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium. Investigative Ophthalmology & Visual Science 2011, 52: 1392-1403. PMID: 21071746, PMCID: PMC3101667, DOI: 10.1167/iovs.10-5984.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsCells, CulturedClaudinsElectric ImpedanceFluorescent Antibody Technique, IndirectGene SilencingGestational AgeHumansImmunoblottingMembrane ProteinsOccludinPermeabilityPolyethylene GlycolsRetinal Pigment EpitheliumReverse Transcriptase Polymerase Chain ReactionRNA, MessengerRNA, Small InterferingTight JunctionsConceptsRetinal pigment epitheliumTransepithelial electrical resistanceHuman retinal pigment epitheliumHuman fetal RPECultured human fetal retinal pigment epitheliumPigment epitheliumClaudin-19Retinal sideHuman fetal retinal pigment epitheliumSpread of edemaFetal retinal pigment epitheliumFetal human retinal pigment epitheliumEffect of serumTight junctionsRPE permeabilityQuantitative RT-PCRChoroidal capillariesSubpopulation of cellsClaudin-1RPE barrierClaudin-3RPE tight junctionsRT-PCREpitheliumSerum