2015
Rapid chemical decontamination of infectious CJD and scrapie particles parallels treatments known to disrupt microbes and biofilms
Botsios S, Tittman S, Manuelidis L. Rapid chemical decontamination of infectious CJD and scrapie particles parallels treatments known to disrupt microbes and biofilms. Virulence 2015, 6: 787-801. PMID: 26556670, PMCID: PMC4826107, DOI: 10.1080/21505594.2015.1098804.Peer-Reviewed Original ResearchConceptsInfectious titerInnate immune responseHuman CJDResistant virusesIatrogenic infectionGT1 cellsBrain changesImmune responseInfectious agentsTSE agentsScrapie agentCJDPrion protein amyloidSheep scrapieVirulent microbesInfectious particlesMin exposureIntrinsic resistanceTreatmentTitersPrP amyloidScrapieAmyloidCultured cellsDeep proteomic analysis
2011
Replication and spread of CJD, kuru and scrapie agents in vivo and in cell culture
Miyazawa K, Emmerling K, Manuelidis L. Replication and spread of CJD, kuru and scrapie agents in vivo and in cell culture. Virulence 2011, 2: 188-199. PMID: 21527829, PMCID: PMC3149681, DOI: 10.4161/viru.2.3.15880.Peer-Reviewed Original ResearchConceptsGT1 cellsSporadic CJDTSE agentsScrapie agentAgent-specific patternsTransmissible spongiform encephalopathy agentsComplex innate immune responseSpongiform encephalopathy agentInnate immune responseHost prion proteinK scrapie agentHuman CJDCJD agentNeuropathological sequelaeBrain titersImmune responseHuman kuruClearance mechanismsCJDInhibitory effectEnvironmental agentsCell-based assaysKuruInfectious formDistinct incubation times
2010
Agent-specific Shadoo Responses in Transmissible Encephalopathies
Miyazawa K, Manuelidis L. Agent-specific Shadoo Responses in Transmissible Encephalopathies. Journal Of Neuroimmune Pharmacology 2010, 5: 155-163. PMID: 20112073, PMCID: PMC2875883, DOI: 10.1007/s11481-010-9191-1.Peer-Reviewed Original ResearchConceptsAgent-specific differencesTransmissible spongiform encephalopathiesGT1 cellsTga20 miceTSE agentsHost prion proteinKuru agentRegional neuropathologyIntracellular amyloidSporadic CJDPrP changesTSE infectionUninfected controlsPrP levelsInfectious agentsAbnormal PrPTransmissible encephalopathiesMiceNeurodegenerative diseasesScrapie agentCJDInfectionInfected cellsSheep scrapieSpongiform encephalopathies
2009
The kuru infectious agent is a unique geographic isolate distinct from Creutzfeldt–Jakob disease and scrapie agents
Manuelidis L, Chakrabarty T, Miyazawa K, Nduom NA, Emmerling K. The kuru infectious agent is a unique geographic isolate distinct from Creutzfeldt–Jakob disease and scrapie agents. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 13529-13534. PMID: 19633190, PMCID: PMC2715327, DOI: 10.1073/pnas.0905825106.Peer-Reviewed Original ResearchConceptsSporadic Creutzfeldt-Jakob diseaseCreutzfeldt-Jakob diseaseBovine spongiform encephalopathyBSE agentHuman sporadic Creutzfeldt-Jakob diseaseInfectious agentsEpidemic bovine spongiform encephalopathyTSE agentsScrapie agentTransmissible spongiform encephalopathy agentsSpongiform encephalopathy agentKuru agentLymphoreticular involvementBrain neuropathologySporadic CJDGT1 cellsNormal miceInfected humansNeurodegenerative diseasesDiseaseViral receptorsInfectious neurodegenerative diseasesSheep scrapieSpongiform encephalopathiesHigh levels
2008
Strain‐specific viral properties of variant Creutzfeldt–Jakob disease (vCJD) are encoded by the agent and not by host prion protein
Manuelidis L, Liu Y, Mullins B. Strain‐specific viral properties of variant Creutzfeldt–Jakob disease (vCJD) are encoded by the agent and not by host prion protein. Journal Of Cellular Biochemistry 2008, 106: 220-231. PMID: 19097123, PMCID: PMC2762821, DOI: 10.1002/jcb.21988.Peer-Reviewed Original ResearchConceptsVariant Creutzfeldt-Jakob diseaseBovine spongiform encephalopathyTransmissible spongiform encephalopathiesVCJD agentTSE strainsInfectious agentsNeuronal culturesEpidemic bovine spongiform encephalopathyMost viral infectionsCreutzfeldt-Jakob diseaseSpongiform encephalopathiesHost prion proteinHost PrP.Human CJDRegional neuropathologyVCJD brainPrion proteinSheep scrapie agentVariant CJDStrain-specific characteristicsBSE strainBrain homogenatesViral infectionPrimate brainScrapie agent