Publications

Selected Publications

Nat Struct Mol Biol 2022

Cryo-EM structures of prefusion SIV envelope trimer

Emilia Krypotou 1 2, Guy E Townsend 1 2 3, Xiaohui Gao 1, Shoichi Tachiyama 1 2, Jun Liu 1 2, Nick D Pokorzynski 1, Andrew L Goodman 1 2, Eduardo A Groisman 1 2. Bacteria require phase separation for fitness in the mammalian gut. Science, 2023.
Kurni Kurniyati, Yunjie Chang, Wangbiao Guo, Jun Liu, Michael G. Malkowski, Chunhao Li. Anti-σ28 Factor FlgM Regulates Flagellin Gene Expression and Flagellar Polarity of Treponema denticola. J Bact, 2023.
Jason Gorman, Chunyan Wang…Jun Liu, Mario Roederer, Peter D Kwong, et al. Cryo-EM structures of prefusion SIV envelope trimer. Nat Struct Mol Biol, 2022.
Donghyun Park, Samuel Steiner, Meng Shao, Craig R. Roy, Jun Liu. Developmental Transitions Coordinate Assembly of the Coxiella burnetii Dot/Icm Type IV Secretion System, Infect Immun, 2022.
Shuaiqi Guo1,2 and Jun Liu1,2*: The Bacterial Flagellar Motor: Insig hts Into Torque Generation, Rotational Switching, and Mechanosensing, Front Microbiol, 2022.
Shoichi Tachiyama, Kar L. Chan, Xiaolin Liu, Skander Hathroubi, Wenwei Li, Briana Peterson, Mohammad F. Khan, Karen M. Ottemann, Jun Liu , and Anna Roujeinikova: The flagellar motor protein FliL forms a scaffold of circumferentially positioned rings required for stator activation, PNAS, 2022.
Shuaiqi Guo, Hui Xu, Yunjie Chang, Md A. Motaleb, and Jun Liu: FliL ring enhances the function of periplasmic flagella, PNAS, 2022. Also highlighted in: How Bacteria Swim: Yale Researchers Discover New Mechanisms
Yunije Chang, Brittany L. Carroll & Jun Liu: Structural basis of bacterial flagellar motor rotation and switching, Trends in Microbiology, 2021.
Donghyun Park, David Chetrit, Bo Hu, Craig R Roy, Jun Liu: Analysis of Dot/Icm Type IVB Secretion System Subassemblies by Cryoelectron Tomography Reveals Conformational Changes Induced by DotB Binding, mBio, 2020.
Brittany L. Carroll and Jun Liu: Structural conservation and adaptation of the bacterial flagellar motor, Biomolecules, 2020.
Yunjie Chang, Kai Zhang, Brittany L. Carroll, Xiaowei Zhao, Nyles W. Charon, Steven J. Norris, Md A. Motaleb, Chunhao Li & Jun Liu: Molecular mechanism for rotational switching of the bacterial flagellar motor, Nat Struct Mol Biol, 2020.
Ze Li, Wenwei Li, Maolin Lu, Julian Bess Jr, Cara W. Chao, Jason Gorman, Daniel S. Terry, Baoshan Zhang, Tongqing Zhou, Scott C. Blanchard, Peter D. Kwong, Jeffrey D . Lifson, Walther Mothes & Jun Liu: Subnanometer structures of HIV-1 envelope trimers on aldrithiol-2-inactivated virus particles, Nat Struct Mol Biol, 2020.
Chang Y, Moon KH, Zhao X, Norris SJ, Motaleb MA, Liu J. Structural insights into flagellar stator-rotor interactions, Elife, 2019.
Wang C, Tu J, Liu J, Molineux IJ. Structural dynamics of bacteriophage P22 infection initiation revealed by cryo-electron tomography, Nat Microbiol, 2019.
Park D, Lara-Tejero M, Waxham MN, Li W, Hu B, Galán JE, Liu J. Visualization of the type III secretion mediated Salmonella-host cell interface using cryo-electron tomography, Elife, 2018.
Zhu S, Nishikino T, Hu B, Kojima S, Homma M, Liu J: Molecular architecture of the sheathed polar flagellum in Vibrio alginolyticus, Proc Natl Acad Sci U S A, 2017.
Hu B, Lara-Tejero M, Kong Q, Galán JE, Liu J: In situ molecular architecture of the Salmonella type III secretion machine, Cell, 2017.
Hu B, Margolin W, Molineux IJ, Liu J, Structural remodeling of bacteriophage T4 and host membranes during infection initiation, Proc Natl Acad Sci U S A, 2015.
Hu B, Morado DR, Margolin W, Rohde JR, Arizmendi O, Picking WL, Picking WD, Liu J: Visualization of the type III secretion sorting platform of Shigella flexneri. Proc Natl Acad Sci U S A, 2015.
Zhao X, Zhang K, Boquoi T, Hu B, Motaleb MA, Miller K, James M, Charon NW, Manson MD, Norris SJ, Li C, Liu J: Cryo-Electron Tomography Reveals the Sequential Assembly of Bacterial Flagella in Borrelia burgdorferi, Proc Natl Acad Sci U S A, 2013.
Hu B, Margolin W, Molineux IJ, Liu J: The Bacteriophage T7 Virion Undergoes Extensive Structural Remodeling during infection, Science, 2013.
Liu J, Hu B, Morado DR, Jani S, Manson MD, Margolin W: Molecular architecture of chemoreceptor arrays revealed by cryoelectron tomography of Escherichia coli minicells, Proc Natl Acad Sci U S A, 2012.
Luther PK, Winkler H, Taylor K, Zoghbi ME, Craig R, Padrón R, Squire JM, Liu J: Direct visualization of myosin-binding protein C bridging myosin and actin filaments in intact muscle, Proc Natl Acad Sci U S A, 2011.
Liu J, Lin T, Botkin DJ, McCrum E, Winkler H, Norris SJ: Intact Flagellar Motor of Borrelia burgdorferi Revealed by Cryo-Electron Tomography: Evidence for Stator Ring Curvature and Rotor/C Ring Assembly Flexion, J Bacteriol, 2009.

All Publications

A pleiotropic role of sialidase in the pathogenicity of Porphyromonas gingivalis.Pham C, Guo S, Han X, Coleman L, Sze CW, Wang H, Liu J, Li C. Infect Immun. 2024 Mar 12; 2024 Feb 20. PMID: 38376159.
Structure of mavacamten-free human cardiac thick filaments within the sarcomere by cryoelectron tomography.Chen L, Liu J, Rastegarpouyani H, Janssen PML, Pinto JR, Taylor KA. Proc Natl Acad Sci U S A. 2024 Feb 27; 2024 Feb 22. PMID: 38386705.
Outer membrane vesicles and the outer membrane protein OmpU govern Vibrio cholerae biofilm matrix assembly.Potapova A, Garvey W, Dahl P, Guo S, Chang Y, Schwechheimer C, Trebino MA, Floyd KA, Phinney BS, Liu J, Malvankar NS, Yildiz FH. mBio. 2024 Feb 14; 2024 Jan 11. PMID: 38206049.
Bacterial flagella hijack type IV pili proteins to control motility.Liu X, Tachiyama S, Zhou X, Mathias RA, Bonny SQ, Khan MF, Xin Y, Roujeinikova A, Liu J, Ottemann KM. Proc Natl Acad Sci U S A. 2024 Jan 23; 2024 Jan 18. PMID: 38236729.
Broadly conserved FlgV controls flagellar assembly and Borrelia burgdorferi dissemination in mice.Zamba-Campero M, Soliman D, Yu H, Lasseter AG, Chang YY, Liu J, Aravind L, Jewett MW, Storz G, Adams PP. bioRxiv. 2024 Jan 9; 2024 Jan 9. PMID: 38260563.
FlgV forms a flagellar motor ring that is required for optimal motility of Helicobacter pylori.Botting JM, Tachiyama S, Gibson KH, Liu J, Starai VJ, Hoover TR. PLoS One. 2023; 2023 Nov 17. PMID: 37976320.
A peek inside the 2023 Bacterial Locomotion and Signal Transduction (BLAST) conference.Hadjifrangiskou M, Liu J, Ottemann KM, Roujeinikova A. Trends Microbiol. 2023 Jun; 2023 Apr 29. PMID: 37127442.
Bacteria require phase separation for fitness in the mammalian gut.Krypotou E, Townsend GE, Gao X, Tachiyama S, Liu J, Pokorzynski ND, Goodman AL, Groisman EA. Science. 2023 Mar 17; 2023 Mar 16. PMID: 36927025.
Anti-σ(28) Factor FlgM Regulates Flagellin Gene Expression and Flagellar Polarity of Treponema denticola.Kurniyati K, Chang Y, Guo W, Liu J, Malkowski MG, Li C. J Bacteriol. 2023 Feb 22; 2023 Jan 30. PMID: 36715541.
Gut colonization by Bacteroides requires translation by an EF-G paralog lacking GTPase activity.Han W, Peng BZ, Wang C, Townsend GE 2nd, Barry NA, Peske F, Goodman AL, Liu J, Rodnina MV, Groisman EA. EMBO J. 2023 Jan 16; 2022 Dec 6. PMID: 36472247.
An essential periplasmic protein coordinates lipid trafficking and is required for asymmetric polar growth in mycobacteria.Gupta KR, Gwin CM, Rahlwes KC, Biegas KJ, Wang C, Park JH, Liu J, Swarts BM, Morita YS, Rego EH. Elife. 2022 Nov 8; 2022 Nov 8. PMID: 36346214.
Cryo-EM structures of prefusion SIV envelope trimer.Gorman J, Wang C, Mason RD, Nazzari AF, Welles HC, Zhou T, Bess JW Jr, Bylund T, Lee M, Tsybovsky Y, Verardi R, Wang S, Yang Y, Zhang B, Rawi R, Keele BF, Lifson JD, Liu J, Roederer M, Kwong PD. Nat Struct Mol Biol. 2022 Nov; 2022 Nov 7. PMID: 36344847.
Developmental Transitions Coordinate Assembly of the Coxiella burnetii Dot/Icm Type IV Secretion System.Park D, Steiner S, Shao M, Roy CR, Liu J. Infect Immun. 2022 Oct 20; 2022 Oct 3. PMID: 36190257.
A Tripartite Efflux System Affects Flagellum Stability in Helicobacter pylori.Gibson K, Chu JK, Zhu S, Nguyen D, Mrázek J, Liu J, Hoover TR. Int J Mol Sci. 2022 Oct 1; 2022 Oct 1. PMID: 36232924.
Editorial: Biological rotary nanomotors.Manson MD, Nan B, Lele PP, Liu J, Duncan TM. Front Microbiol. 2022; 2022 Sep 21. PMID: 36212881.
Identification and Characterization of the Alternative σ(28) Factor in Treponema denticola.Kurniyati K, Chang Y, Liu J, Li C. J Bacteriol. 2022 Sep 20; 2022 Aug 31. PMID: 36043861.
Transcriptome of Epibiont Saccharibacteria Nanosynbacter lyticus Strain TM7x During the Establishment of Symbiosis.Hendrickson EL, Bor B, Kerns KA, Lamont EI, Chang Y, Liu J, Cen L, Schulte F, Hardt M, Shi W, He X, McLean JS. J Bacteriol. 2022 Sep 20; 2022 Aug 17. PMID: 35975994.
Transcriptional and functional characterizations of multiple flagellin genes in spirochetes.Kurniyati K, Chang Y, Liu J, Li C. Mol Microbiol. 2022 Sep; 2022 Jul 18. PMID: 35776658.
The Bacterial Flagellar Motor: Insights Into Torque Generation, Rotational Switching, and Mechanosensing.Guo S, Liu J. Front Microbiol. 2022; 2022 May 30. PMID: 35711788.
Spindle-shaped archaeal viruses evolved from rod-shaped ancestors to package a larger genome.Wang F, Cvirkaite-Krupovic V, Vos M, Beltran LC, Kreutzberger MAB, Winter JM, Su Z, Liu J, Schouten S, Krupovic M, Egelman EH. Cell. 2022 Apr 14; 2022 Mar 23. PMID: 35325592.
FliL ring enhances the function of periplasmic flagella.Guo S, Xu H, Chang Y, Motaleb MA, Liu J. Proc Natl Acad Sci U S A. 2022 Mar 15; 2022 Mar 7. PMID: 35254893.
The flagellar motor protein FliL forms a scaffold of circumferentially positioned rings required for stator activation.Tachiyama S, Chan KL, Liu X, Hathroubi S, Peterson B, Khan MF, Ottemann KM, Liu J, Roujeinikova A. Proc Natl Acad Sci U S A. 2022 Jan 25. PMID: 35046042.
Characterization of the Flagellar Collar Reveals Structural Plasticity Essential for Spirochete Motility.Chang Y, Xu H, Motaleb MA, Liu J. mBio. 2021 Dec 21; 2021 Nov 23. PMID: 34809456.
Role of the major determinant of polar flagellation FlhG in the endoflagella-containing spirochete Leptospira.Fule L, Halifa R, Fontana C, Sismeiro O, Legendre R, Varet H, Coppée JY, Murray GL, Adler B, Hendrixson DR, Buschiazzo A, Guo S, Liu J, Picardeau M. Mol Microbiol. 2021 Nov; 2021 Oct 30. PMID: 34657338.
Structural basis of bacterial flagellar motor rotation and switching.Chang Y, Carroll BL, Liu J. Trends Microbiol. 2021 Nov; 2021 Apr 14. PMID: 33865677.
BB0259 Encompasses a Peptidoglycan Lytic Enzyme Function for Proper Assembly of Periplasmic Flagella in Borrelia burgdorferi.Xu H, Hu B, Flesher DA, Liu J, Motaleb MA. Front Microbiol. 2021; 2021 Oct 1. PMID: 34659138.
Interconnecting solvent quality, transcription, and chromosome folding in Escherichia coli.Xiang Y, Surovtsev IV, Chang Y, Govers SK, Parry BR, Liu J, Jacobs-Wagner C. Cell. 2021 Jul 8; 2021 Jun 28. PMID: 34186018.
Composition and Biophysical Properties of the Sorting Platform Pods in the Shigella Type III Secretion System.Tachiyama S, Skaar R, Chang Y, Carroll BL, Muthuramalingam M, Whittier SK, Barta ML, Picking WL, Liu J, Picking WD. Front Cell Infect Microbiol. 2021; 2021 Jun 3. PMID: 34150677.
The peptidoglycan-associated protein NapA plays an important role in the envelope integrity and in the pathogenesis of the lyme disease spirochete.Davis MM, Brock AM, DeHart TG, Boribong BP, Lee K, McClune ME, Chang Y, Cramer N, Liu J, Jones CN, Jutras BL. PLoS Pathog. 2021 May; 2021 May 13. PMID: 33984073.
A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi.Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C. Appl Environ Microbiol. 2021 Mar 1; 2020 Nov 30. PMID: 33257311.
Symmetrical arrangement of proteins under release-ready vesicles in presynaptic terminals.Radhakrishnan A, Li X, Grushin K, Krishnakumar SS, Liu J, Rothman JE. Proc Natl Acad Sci U S A. 2021 Feb 2. PMID: 33468631.
Molecular mechanism for rotational switching of the bacterial flagellar motor.Chang Y, Zhang K, Carroll BL, Zhao X, Charon NW, Norris SJ, Motaleb MA, Li C, Liu J. Nat Struct Mol Biol. 2020 Nov; 2020 Sep 7. PMID: 32895555.
Structural Conservation and Adaptation of the Bacterial Flagella Motor.Carroll BL, Liu J. Biomolecules. 2020 Oct 29; 2020 Oct 29. PMID: 33138111.
The flagellar motor of Vibrio alginolyticus undergoes major structural remodeling during rotational switching.Carroll BL, Nishikino T, Guo W, Zhu S, Kojima S, Homma M, Liu J. Elife. 2020 Sep 7; 2020 Sep 7. PMID: 32893817.
Subnanometer structures of HIV-1 envelope trimers on aldrithiol-2-inactivated virus particles.Li Z, Li W, Lu M, Bess J Jr, Chao CW, Gorman J, Terry DS, Zhang B, Zhou T, Blanchard SC, Kwong PD, Lifson JD, Mothes W, Liu J. Nat Struct Mol Biol. 2020 Aug; 2020 Jun 29. PMID: 32601441.
Seeing a Contractile Bactericidal Nanomachine in Action at Near-Atomic Resolution.Gong Q, Liu J. Biochemistry. 2020 Jun 23; 2020 Jun 9. PMID: 32517471.
Establishing rod shape from spherical, peptidoglycan-deficient bacterial spores.Zhang H, Mulholland GA, Seef S, Zhu S, Liu J, Mignot T, Nan B. Proc Natl Acad Sci U S A. 2020 Jun 23; 2020 Jun 8. PMID: 32513721.
FlhF regulates the number and configuration of periplasmic flagella in Borrelia burgdorferi.Zhang K, He J, Cantalano C, Guo Y, Liu J, Li C. Mol Microbiol. 2020 Jun; 2020 Feb 21. PMID: 32039533.
Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System.Chetrit D, Park D, Hu B, Liu J, Roy CR. J Vis Exp. 2020 Mar 10; 2020 Mar 10. PMID: 32225141.
Phylogenetic Distribution, Ultrastructure, and Function of Bacterial Flagellar Sheaths.Chu J, Liu J, Hoover TR. Biomolecules. 2020 Feb 27; 2020 Feb 27. PMID: 32120823.
Analysis of Dot/Icm Type IVB Secretion System Subassemblies by Cryoelectron Tomography Reveals Conformational Changes Induced by DotB Binding.Park D, Chetrit D, Hu B, Roy CR, Liu J. mBio. 2020 Feb 18; 2020 Feb 18. PMID: 32071271.
BB0326 is responsible for the formation of periplasmic flagellar collar and assembly of the stator complex in Borrelia burgdorferi.Xu H, He J, Liu J, Motaleb MA. Mol Microbiol. 2020 Feb; 2019 Dec 8. PMID: 31743518.
In Situ Structure of the Vibrio Polar Flagellum Reveals a Distinct Outer Membrane Complex and Its Specific Interaction with the Stator.Zhu S, Nishikino T, Takekawa N, Terashima H, Kojima S, Imada K, Homma M, Liu J. J Bacteriol. 2020 Jan 29; 2020 Jan 29. PMID: 31767780.
The cytoplasmic domain of MxiG interacts with MxiK and directs assembly of the sorting platform in the Shigella type III secretion system.Tachiyama S, Chang Y, Muthuramalingam M, Hu B, Barta ML, Picking WL, Liu J, Picking WD. J Biol Chem. 2019 Dec 13; 2019 Nov 7. PMID: 31699894.
High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway.Butan C, Lara-Tejero M, Li W, Liu J, Galán JE. Proc Natl Acad Sci U S A. 2019 Dec 3; 2019 Nov 19. PMID: 31744874.
Loss of a Cardiolipin Synthase in Helicobacter pylori G27 Blocks Flagellum Assembly.Chu JK, Zhu S, Herrera CM, Henderson JC, Liu J, Trent MS, Hoover TR. J Bacteriol. 2019 Nov 1; 2019 Oct 4. PMID: 31427391.
Structural insights into flagellar stator-rotor interactions.Chang Y, Moon KH, Zhao X, Norris SJ, Motaleb MA, Liu J. Elife. 2019 Jul 17; 2019 Jul 17. PMID: 31313986.
Architecture and Assembly of Periplasmic Flagellum.Chang Y, Liu J. Microbiol Spectr. 2019 Jul. PMID: 31373267.
In Situ Structures of Polar and Lateral Flagella Revealed by Cryo-Electron Tomography.Zhu S, Schniederberend M, Zhitnitsky D, Jain R, Galán JE, Kazmierczak BI, Liu J. J Bacteriol. 2019 Jul 1; 2019 Jun 10. PMID: 31010901.
Structural dynamics of bacteriophage P22 infection initiation revealed by cryo-electron tomography.Wang C, Tu J, Liu J, Molineux IJ. Nat Microbiol. 2019 Jun; 2019 Mar 18. PMID: 30886360.
Analysis of a flagellar filament cap mutant reveals that HtrA serine protease degrades unfolded flagellin protein in the periplasm of Borrelia burgdorferi.Zhang K, Qin Z, Chang Y, Liu J, Malkowski MG, Shipa S, Li L, Qiu W, Zhang JR, Li C. Mol Microbiol. 2019 Jun; 2019 Apr 26. PMID: 30883947.
A pleiotropic role of FlaG in regulating the cell morphogenesis and flagellar homeostasis at the cell poles of Treponema denticola.Kurniyati K, Liu J, Zhang JR, Min Y, Li C. Cell Microbiol. 2019 Feb; 2018 Jul 23. PMID: 29935042.
Cryo-electron tomography of periplasmic flagella in Borrelia burgdorferi reveals a distinct cytoplasmic ATPase complex.Qin Z, Tu J, Lin T, Norris SJ, Li C, Motaleb MA, Liu J. PLoS Biol. 2018 Nov; 2018 Nov 9. PMID: 30412577.
A tetratricopeptide repeat domain protein has profound effects on assembly of periplasmic flagella, morphology and motility of the lyme disease spirochete Borrelia burgdorferi.Moon KH, Zhao X, Xu H, Liu J, Motaleb MA. Mol Microbiol. 2018 Nov; 2018 Oct 15. PMID: 30303576.
The Vibrio H-Ring Facilitates the Outer Membrane Penetration of the Polar Sheathed Flagellum.Zhu S, Nishikino T, Kojima S, Homma M, Liu J. J Bacteriol. 2018 Nov 1; 2018 Oct 10. PMID: 30104237.
Visualization of the type III secretion mediated Salmonella-host cell interface using cryo-electron tomography.Park D, Lara-Tejero M, Waxham MN, Li W, Hu B, Galán JE, Liu J. Elife. 2018 Oct 3; 2018 Oct 3. PMID: 30281019.
A unique cytoplasmic ATPase complex defines the Legionella pneumophila type IV secretion channel.Chetrit D, Hu B, Christie PJ, Roy CR, Liu J. Nat Microbiol. 2018 Jun; 2018 May 21. PMID: 29784975.
Visualizing Chemoreceptor Arrays in Bacterial Minicells by Cryo-Electron Tomography and Subtomogram Analysis.Qin Z, Hu B, Liu J. Methods Mol Biol. 2018. PMID: 29429093.
Molecular architecture of the sheathed polar flagellum in Vibrio alginolyticus.Zhu S, Nishikino T, Hu B, Kojima S, Homma M, Liu J. Proc Natl Acad Sci U S A. 2017 Oct 10; 2017 Sep 25. PMID: 28973904.