2020
Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells
Benedetti L, Marvin JS, Falahati H, Guillén-Samander A, Looger LL, De Camilli P. Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells. ELife 2020, 9: e63230. PMID: 33174843, PMCID: PMC7735757, DOI: 10.7554/elife.63230.Peer-Reviewed Original ResearchConceptsProtein fusion partnersSmall subcellular volumesSubcellular optogeneticsOrganelle contactsHomodimerization interfaceProtein modulesMammalian cellsBiological processesPhysiological processesSubcellular organellesLow temperature preincubationSimultaneous photoactivationFusion partnerCell preincubationWhole cellsSubcellular volumesConcatemerizationSpatial controlSpatiotemporal confinementCellsNeurosporaOrganellesHeterodimersVIVIDProtein
2010
Cloning, Expression, and Characterization of a Novel Methylglyoxal Synthase from Thermus sp. Strain GH5
Pazhang M, Khajeh K, Asghari SM, Falahati H, Naderi-Manesh H. Cloning, Expression, and Characterization of a Novel Methylglyoxal Synthase from Thermus sp. Strain GH5. Applied Biochemistry And Biotechnology 2010, 162: 1519-1528. PMID: 20419481, DOI: 10.1007/s12010-010-8933-0.Peer-Reviewed Original ResearchConceptsMethylglyoxal synthaseThermus spAmino acid sequenceGel filtration experimentsAcid sequenceHexameric enzymeAllosteric inhibitorsQuaternary structureEnzyme subunitMolecular massAmino acidsArg-150GH5Q-SepharoseGenesTMGSSpEnzymeSynthasePresence of phosphateOptimum activityHill coefficientCloningSubunitsPolypeptide