Skip to Main Content
The De Camilli Lab
YSM Home

INFORMATION FOR

Multimedia

Images: Experiments and Findings

  • 1_amphiphysin tubules

    Tubulation of a liposome by the N-BAR domain of amphiphysin (see: Takei K, Slepnev VI, Haucke V, and De Camilli P. 1999. Nat. Cell Biol. 1: 33-39)

  • 2_dynamin 13 DKO

    Tomographic EM reconstruction of plasma membrane invaginations enriched in clathrin coated pits at a nerve terminal lacking dynamin 1 and 3. (from: Raimondi A, Ferguson SM, Lou X, Armbruster M, Paradise S, Giovedi S, Messa M, Kono N, Takashi J, Cappello V, O’Toole E, Ryan TA, and De Camilli P. 2010. Neuron. 70(6):1100-14.)

  • 3_membrane sheet EM

    Endocytic intermediates generated by the incubation of isolated plasma membrane sheets with brain cytosol. (see: Wu M, Huang B, Graham M, Raimondi A, Heuser JE, Zhuang X, and De Camilli P. 2010. Nat. Cell Biol. 9: 902-908.)

  • 4_z_dynamin KO EM

    Electron micrograph of a synapse lacking dynamin 1. Note abundance of clathrin coated profiles. (see: Ferguson SM, Brasnjo G, Hayashi M, Wölfel M, Collesi C, Giovedi S, Raimondi A, Gong LW, Paradise S, O’Toole E, Flavell R, Cremona O, Miesenböck, Ryan TA, and De Camilli P. 2007. Science. 316: 570-574.)

  • 5_synapses WT dynKO sjKO

    Tomographic EM reconstruction of thick sections of synapses from wild type mouse neurons (left), dynamin 1 KO neurons (center) and synaptojanin 1 KO neurons (right). Blue = synaptic vesicles; green = clathrin coated profiles. (from: Hayashi M, Raimondi A, O’Toole E, Paradise S, Collesi C, Cremona O, Ferguson SM, and De Camilli P. 2008. Proc. Nat. Acad. Sci. 105: 2175-2180.)

  • 6_synapse EM

    Electron microscopic image of a synapse in the mouse cerebellum

  • 7_dynamin12DKO fibroblast EM

    Electron tomogram (left) and corresponding reconstruction of tubular plasma membrane invaginations capped by clathrin coated pits (red) in a fibroblast the lack dynamin. (from: Ferguson S, Raimondi A, Paradise S, Shen H, Mesaki K, Destaing O, Ko G, Cremona O, O'Toole E, and De Camilli P. 2009. Dev. Cell. 17: 811-822.)

  • 8_neuron staining

    Immunofluorescence of neurons in culture: presynaptic nerve terminals are visualized by anti-synapsin antibodies (green) (from the work of Shawn Ferguson)

  • 9_dynamin13DKO synapse

    Electron micrograph of a synapse lacking both dynamin 1 and 3. Note the abundance of clathrin coated profiles. (from: Raimondi A, Ferguson SM, Lou X, Armbruster M, Paradise S, Giovedi S, Messa M, Kono N, Takashi J, Cappello V, O’Toole E, Ryan TA, and De Camilli P. 2010. Neuron. 70(6):1100-14.)

  • 10_VPS13A and VPS13C localization

    VPS13A and VPS13C are localized at contacts between the ER and either mitochondria or endo/lysosomes respectively. Top: schematic representation showing that both proteins can also localize at contacts between the ER and lipid droplets. Bottom fluorescence image (green: ER; blue: mitochondria or endo/lysosomes respectively; magenta: VPS13)(Kumar, Leonzino et al. 2018, J. Cell Biol 2018, PMID:30093493)2025 Yale School of Medicine. Updated 12/12/2025