2024
Decoding transcriptomic signatures of cysteine string protein alpha–mediated synapse maintenance
Wang N, Zhu B, Allnutt M, Grijalva R, Zhao H, Chandra S. Decoding transcriptomic signatures of cysteine string protein alpha–mediated synapse maintenance. Proceedings Of The National Academy Of Sciences Of The United States Of America 2024, 121: e2320064121. PMID: 38833477, PMCID: PMC11181078, DOI: 10.1073/pnas.2320064121.Peer-Reviewed Original ResearchConceptsSynapse maintenanceTranscriptional changesSynaptogenic adhesion moleculesGene ontology analysisKO miceKO brainMaintenance in vivoCell-cell interactionsGlial cellsSingle-nucleus transcriptomesOntology analysisCspADifferential expressionNeuron-glia interactionsAutophagy-related genesProtein AGenesCell typesNeurodegenerative diseasesInhibitory synapsesLittermate controlsSynaptic pathwaysAdhesion moleculesGlial responseSynapse
2023
cAMP−EPAC−PKCε−RIM1α signaling regulates presynaptic long-term potentiation and motor learning
Wang X, Zhou L, Dong B, Xu F, Wang D, Shen E, Cai X, Wang Y, Wang N, Ji S, Chen W, Schonewille M, Zhu J, De Zeeuw C, Shen Y. cAMP−EPAC−PKCε−RIM1α signaling regulates presynaptic long-term potentiation and motor learning. ELife 2023, 12: e80875. PMID: 37159499, PMCID: PMC10171863, DOI: 10.7554/elife.80875.Peer-Reviewed Original ResearchConceptsPresynaptic long-term potentiationSignaling cascadesPresynaptic plasticityRelease of synaptic vesiclesLong-term potentiationThreonine phosphorylationSynaptic vesiclesFunctional relevancePurkinje cell synapsesMotor behaviorPresynaptic formLearning mechanismCerebellar learning mechanismsCascadeLearningCell synapsesBasic performanceMotor learningThreonineMotor skillsSignalVesicles
2022
KIF2C regulates synaptic plasticity and cognition in mice through dynamic microtubule depolymerization
Zheng R, Du Y, Wang X, Liao T, Zhang Z, Wang N, Li X, Shen Y, Shi L, Luo J, Xia J, Wang Z, Xu J. KIF2C regulates synaptic plasticity and cognition in mice through dynamic microtubule depolymerization. ELife 2022, 11: e72483. PMID: 35138249, PMCID: PMC8828051, DOI: 10.7554/elife.72483.Peer-Reviewed Original ResearchConceptsDynamic microtubulesFunctional plasticity of synapsesRegulate synaptic developmentConditional knockout approachFunction of KIF2CNervous systemNeuronal activity-dependent mannerPlasticity of synapsesProtein traffickingActivity-dependent mannerDepolymerizing proteinsRNAi knockdownSynaptic plasticityExpression of AMPA receptorsMicrotubule dynamicsMicrotubule invasionMicrotubule depolymerizationImpaired excitatory transmissionKnockout approachRegulatory mechanismsKIF2CDepolymerization capabilityLong-term potentiationSynaptic developmentMicrotubules
2020
Gab1 mediates PDGF signaling and is essential to oligodendrocyte differentiation and CNS myelination
Zhou L, Shao C, Xie Y, Wang N, Xu S, Luo B, Wu Z, Ke Y, Qiu M, Shen Y. Gab1 mediates PDGF signaling and is essential to oligodendrocyte differentiation and CNS myelination. ELife 2020, 9: e52056. PMID: 31944179, PMCID: PMC6984811, DOI: 10.7554/elife.52056.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCateninsCell DifferentiationCell LineageCentral Nervous SystemGene Expression RegulationMiceMice, KnockoutOligodendrocyte Precursor CellsOligodendrogliaPlatelet-Derived Growth FactorProtein-Tyrosine KinasesReceptors, Growth FactorRNA, Small InterferingSignal TransductionTranscription FactorsTranscriptomeConceptsPlatelet-derived growth factorCentral nervous system myelinDownstream targetsPlatelet-derived growth factor stimulationPDGF signalingPlatelet-derived growth factor signalingReceptor tyrosine kinasesAdaptor proteinCentral nervous system hypomyelinationGab1Nuclear accumulationOligodendrocyte precursor cellsTyrosine kinaseCentral nervous systemOligodendrocyte precursor cell differentiationConditional deletionOL lineage cellsOligodendrocyte differentiationDifferentiationSteady-state numberB-cateninPrecursor cellsLineage cellsTrophic supportGrowth factor
2019
Numb deficiency causes impaired trafficking of mGlu5 in neurons and autistic-like behaviors
Wang N, Wang D, Shen Y. Numb deficiency causes impaired trafficking of mGlu5 in neurons and autistic-like behaviors. Neuroscience Letters 2019, 707: 134291. PMID: 31129078, DOI: 10.1016/j.neulet.2019.134291.Peer-Reviewed Original ResearchConceptsMembrane expressionExpression of metabotropic glutamate receptor 1Expression of metabotropic glutamate receptor 5Metabotropic glutamate receptor 1Expression of mGlu5Metabotropic glutamate receptor 5Clathrin-coated vesiclesGlutamate receptor 1Functions of NumbGlutamate receptor 5Three-chamber social testNumb deficiencyAutistic-like behaviorsTrans-membrane receptorsHomologous genesExcitatory neuronsReceptor 5MGlu5Receptor 1Impaired traffickingConditional knockoutNumbnessBrain regionsSocial testMotor coordination
2018
Celecoxib Ameliorates Seizure Susceptibility in Autosomal Dominant Lateral Temporal Epilepsy
Zhou L, Zhou L, Su L, Cao S, Xie Y, Wang N, Shao C, Wang Y, Zhou J, Cowell J, Shen Y. Celecoxib Ameliorates Seizure Susceptibility in Autosomal Dominant Lateral Temporal Epilepsy. Journal Of Neuroscience 2018, 38: 3346-3357. PMID: 29491011, PMCID: PMC5884462, DOI: 10.1523/jneurosci.3245-17.2018.Peer-Reviewed Original ResearchConceptsLeucine-rich glioma-inactivated 1Autosomal dominant lateral temporal epilepsyIntrinsic excitability of pyramidal neuronsExcitability of pyramidal neuronsGlioma-inactivated 1Seizure susceptibilityPyramidal neuronsIntrinsic excitabilityLateral temporal epilepsyGlutamatergic transmissionInherited syndromeTemporal epilepsyNonsynaptic epileptiform activityCytosolic phospholipase A<sub>2</sub>Nonsteroidal anti-inflammatory drugsCortical pyramidal neuronsAnti-inflammatory drugsKv1.2 expressionEpileptiform activityMutant miceFDA-approved drugsPathogenic basisCOX2 inhibitionCortical neuronsCelecoxibPotassium channels-mediated electrophysiologic responses are inhibited by cytosolic phospholipase A2&agr; ablation
Wang N, Hu Y, Su L. Potassium channels-mediated electrophysiologic responses are inhibited by cytosolic phospholipase A2&agr; ablation. Neuroreport 2018, 29: 59-64. PMID: 29112675, DOI: 10.1097/wnr.0000000000000933.Peer-Reviewed Original ResearchConceptsA-type K channelsK channelsCA1 neuronsCa sensitivitySimultaneous patch-clamp recordingsHippocampal CA1 pyramidal neuronsPatch-clamp recordingsCA1 pyramidal neuronsExcitotoxic neuronal injuryElectrophysiological responsesCa transientsPyramidal neuronsNeuronal injuryCA imagingCerebral ischemiaCPLA2aElectrophysiological eventsWild-typeNeuronsCA1MiceBlockersA2AIschemiaInjury
2016
Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons
Xu J, Wang N, Luo J, Xia J. Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons. Scientific Reports 2016, 6: 20924. PMID: 26868290, PMCID: PMC4751430, DOI: 10.1038/srep20924.Peer-Reviewed Original ResearchMeSH KeywordsAcid Sensing Ion ChannelsAnimalsAxonsCarrier ProteinsChlorocebus aethiopsCOS CellsCytoskeletal ProteinsGene Knockdown TechniquesHEK293 CellsHumansIntracellular Signaling Peptides and ProteinsMembrane ProteinsMicrotubule-Associated ProteinsMicrotubulesNeuronsNuclear ProteinsProtein BindingProtein TransportRatsTransport VesiclesConceptsMembrane proteinsKinesin-binding proteinPeripheral membrane proteinsDiverse membrane proteinsVesicle traffickingGolgi complexSyntabulinMembrane localizationSynaptic vesiclesTrafficking dynamicsMicrotubule structurePICK1TraffickingPICK1 expressionMetabotropic glutamate receptor 7VesiclesProteinMicrotubulesProtein expressionCell systemExpressionGolgiMitochondriaSynaptic receptorsReceptors
2015
Functions of Kinesin Superfamily Proteins in Neuroreceptor Trafficking
Wang N, Xu J. Functions of Kinesin Superfamily Proteins in Neuroreceptor Trafficking. BioMed Research International 2015, 2015: 639301. PMID: 26075252, PMCID: PMC4449888, DOI: 10.1155/2015/639301.Peer-Reviewed Original ResearchConceptsKinesin superfamily proteinsSuperfamily proteinsSynaptic plasticityTransport various cargoesGlutamate receptorsKainate receptorsGABA receptorsAdaptor proteinTrafficking of NMDA receptorsN-methyl-D-aspartate-Cellular basis of learningMolecular mechanismsMolecular eventsTraffickingProteinNMDA receptorsAMPA receptorsSynaptic transmissionCellular basisNeuronal activityReceptorsBasis of learningKainateGABAAdaptor
2012
AMPA Receptors Regulate Exocytosis and Insulin Release in Pancreatic β Cells
Wu Z, Zhu L, Zou N, Bombek L, Shao C, Wang N, Wang X, Liang L, Xia J, Rupnik M, Shen Y. AMPA Receptors Regulate Exocytosis and Insulin Release in Pancreatic β Cells. Traffic 2012, 13: 1124-1139. PMID: 22540213, DOI: 10.1111/j.1600-0854.2012.01373.x.Peer-Reviewed Original ResearchConceptsK(ATP) channelsGlutamate applicationB cellsInsulin releaseA-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptorIonotropic glutamate receptorsKnock-out miceDepolarization-induced exocytosisMouse B cellsInsulin-containing granulesPancreatic B-cellsGlutamate receptorsCytosolic calciumCytosolic cGMPAMPARAcid receptorsInsulin secretionInsulinoma cellsInsulinGlutamateMiceReceptorsExocytosisIGluRsRelease