Applied Biosystems (ABI)

The ABI PRISM 7900HT Sequence Detection System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5' nuclease assay.

Fluorescence Detection

To induce fluorescence, the 7900HT system distributes light from an argon-ion laser excitation source to all sample wells. It then directs the resulting fluorescent emission through a spectrograph to a charge-coupled device (CCD) camera. Emission wavelenghts from 500-600 nm are monitored allowing the simultaneous detection of multiple fluorophores. The 7900HT instrumentation is seen below.