2021
3D super-resolution deep-tissue imaging in living mice
Velasco MGM, Zhang M, Antonello J, Yuan P, Allgeyer ES, May D, M’Saad O, Kidd P, Barentine AES, Greco V, Grutzendler J, Booth MJ, Bewersdorf J. 3D super-resolution deep-tissue imaging in living mice. Optica 2021, 8: 442-450. PMID: 34239948, PMCID: PMC8243577, DOI: 10.1364/optica.416841.Peer-Reviewed Original ResearchWater-immersion objective lensTwo-photon excitationSuper-resolution imagingEmission depletion (STED) microscopyAdaptive opticsSTED systemObjective lensOptical aberrationsDepletion microscopyBiological tissuesNanoscale structuresLiving mouseOrganic dyesOpticsSTEDExcitationMouse brain tissueLiving cellsThree-dimensional visualizationMicroscopyLightUnique insightsImagingLens
2020
Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging
Zhang Y, Schroeder LK, Lessard MD, Kidd P, Chung J, Song Y, Benedetti L, Li Y, Ries J, Grimm JB, Lavis LD, De Camilli P, Rothman JE, Baddeley D, Bewersdorf J. Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging. Nature Methods 2020, 17: 225-231. PMID: 31907447, PMCID: PMC7028321, DOI: 10.1038/s41592-019-0676-4.Peer-Reviewed Original ResearchConceptsSuper-resolution microscopeProtein complexesNanoscale resolutionSubcellular architectureMammalian cellsCellular organellesPlasma membraneGolgi apparatusEndoplasmic reticulumElectron microscopyThree-dimensional imagingFluorescence approachMulticolor imagingSpatial organizationLocalization precisionFluorescence imagingMolecular specificityFluorescence imagesMicroscopeFluorescent probeOrganellesReticulumMicroscopyImagingMembrane