2002
Src Kinase Mediates Phosphatidylinositol 3-Kinase/Akt-dependent Rapid Endothelial Nitric-oxide Synthase Activation by Estrogen*
Haynes MP, Li L, Sinha D, Russell KS, Hisamoto K, Baron R, Collinge M, Sessa WC, Bender JR. Src Kinase Mediates Phosphatidylinositol 3-Kinase/Akt-dependent Rapid Endothelial Nitric-oxide Synthase Activation by Estrogen*. Journal Of Biological Chemistry 2002, 278: 2118-2123. PMID: 12431978, DOI: 10.1074/jbc.m210828200.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsBlotting, WesternCell LineCells, CulturedElectrophoresis, Polyacrylamide GelEndoplasmic ReticulumEndothelium, VascularEnzyme ActivationEnzyme InhibitorsEstrogensHumansMiceMutationNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IINitric Oxide Synthase Type IIIPhosphatidylinositol 3-KinasesPhosphorylationPrecipitin TestsProtein BindingProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktReceptors, EstrogenSignal TransductionSrc-Family KinasesTime FactorsTransfectionTyrosineConceptsC-SrcPI3-kinaseAkt phosphorylationSrc kinaseUpstream regulatorKinase-dead c-SrcC-Src associationActive c-SrcC-Src phosphorylationMurine embryonic fibroblastsBasal Akt phosphorylationC-Src expressionCritical upstream regulatorEndothelial nitric oxide synthaseSrc familyActive AktEmbryonic fibroblastsComplex formation resultsEndothelial cellsHuman endothelial cellsAkt activationPhosphorylationKinaseAktPhosphatidylinositol
1995
Conserved regions in the cytoplasmic domains of the leukocyte integrin alpha L beta 2 are involved in endoplasmic reticulum retention, dimerization, and cytoskeletal association.
Pardi R, Bossi G, Inverardi L, Rovida E, Bender J. Conserved regions in the cytoplasmic domains of the leukocyte integrin alpha L beta 2 are involved in endoplasmic reticulum retention, dimerization, and cytoskeletal association. The Journal Of Immunology 1995, 155: 1252-63. PMID: 7636193, DOI: 10.4049/jimmunol.155.3.1252.Peer-Reviewed Original ResearchMeSH KeywordsAllosteric RegulationAmino Acid SequenceAnimalsCell CompartmentationCell Line, TransformedChlorocebus aethiopsCytoskeletonEndoplasmic ReticulumLymphocyte Function-Associated Antigen-1Molecular Sequence DataProtein ConformationProtein MultimerizationProtein Structure, TertiaryRecombinant Fusion ProteinsSequence DeletionT-LymphocytesTetradecanoylphorbol AcetateTransfectionConceptsAlpha L beta 2Cytoplasmic domainEndoplasmic reticulum retentionCytoskeletal associationBeta subunitAlpha L subunitAlpha beta complexFunction of integrinsBeta 2GFFKR motifAdherent cellsDeletion mutantsSubstitution mutantsEctopic expressionIntegrin alphaPlasma membraneHeterodimer formationConserved regionsAdhesion receptorsBeta complexCytoplasmic truncationRegulated functionsL subunitsStructural determinantsAlpha L