2017
Mechanisms of bile acid mediated inflammation in the liver
Li M, Cai SY, Boyer JL. Mechanisms of bile acid mediated inflammation in the liver. Molecular Aspects Of Medicine 2017, 56: 45-53. PMID: 28606651, PMCID: PMC5662014, DOI: 10.1016/j.mam.2017.06.001.Peer-Reviewed Original ResearchConceptsLiver injuryBile acidsCholestatic animal modelsCauses of cholestasisCholestatic liver injuryInnate immune cellsBiliary injuryNeutrophil recruitmentBile flowImmune cellsEffective therapyInflammatory responseAnimal modelsMolecular mediatorsCholestasisInjuryNovel targetLiverElevated levelsPathological processesMolecular mechanismsHepatocytesInflammationPatientsPathogenesis
2015
Canalicular membrane MRP2/ABCC2 internalization is determined by Ezrin Thr567 phosphorylation in human obstructive cholestasis
Chai J, Cai SY, Liu X, Lian W, Chen S, Zhang L, Feng X, Cheng Y, He X, He Y, Chen L, Wang R, Wang H, Boyer JL, Chen W. Canalicular membrane MRP2/ABCC2 internalization is determined by Ezrin Thr567 phosphorylation in human obstructive cholestasis. Journal Of Hepatology 2015, 63: 1440-1448. PMID: 26212029, PMCID: PMC4686151, DOI: 10.1016/j.jhep.2015.07.016.Peer-Reviewed Original ResearchMeSH KeywordsAdultBile CanaliculiCase-Control StudiesCholestasisCytoskeletal ProteinsFemaleGallstonesGene Knockdown TechniquesHep G2 CellsHumansLiverMaleMembrane ProteinsMiddle AgedModels, BiologicalMultidrug Resistance-Associated Protein 2Multidrug Resistance-Associated ProteinsPhosphorylationProtein Kinase CReceptors, Autocrine Motility FactorRNA, MessengerThreonineConceptsObstructive cholestasisCholestatic liverMRP2 expressionMrp2 internalizationHepG2 cellsHuman obstructive cholestasisMRP2 protein expressionMembrane expressionHepatic MRP2 expressionNon-cholestatic controlsExpression of PKCαTotal protein levelsBile ductCholestatic patientsCholestasisBile acidsPatientsAbstractTextHuman liverProtein expressionProtein levelsLiverMRP2JaundiceAIMS
2006
The organic anion transport polypeptide 1d1 (Oatp1d1) mediates hepatocellular uptake of phalloidin and microcystin into skate liver
Meier-Abt F, Hammann-Hänni A, Stieger B, Ballatori N, Boyer J. The organic anion transport polypeptide 1d1 (Oatp1d1) mediates hepatocellular uptake of phalloidin and microcystin into skate liver. Toxicology And Applied Pharmacology 2006, 218: 274-279. PMID: 17198718, DOI: 10.1016/j.taap.2006.11.015.Peer-Reviewed Original ResearchConceptsSkate liverToxic liver injuryFunctional transport activityLiver-specific OATPsOrganic anion transporting polypeptidesLiver injuryOrganic anion transportersWater-injected oocytesXenobiotic toxinsHepatocellular uptakeBile saltsSkate hepatocytesOATP substratesHuman brainLiverPresent studyOatps/OATPsAnion transportersOATPDemethylphalloinHepatocytesTransport activityToxinCellular uptakeUptake
2001
Cellular localization and up‐regulation of multidrug resistance–associated protein 3 in hepatocytes and cholangiocytes during obstructive cholestasis in rat liver
Soroka C, Lee J, Azzaroli F, Boyer J. Cellular localization and up‐regulation of multidrug resistance–associated protein 3 in hepatocytes and cholangiocytes during obstructive cholestasis in rat liver. Hepatology 2001, 33: 783-791. PMID: 11283840, DOI: 10.1053/jhep.2001.23501.Peer-Reviewed Original ResearchConceptsObstructive cholestasisMultidrug resistance-associated protein 3Bile duct-ligated animalsMultidrug resistanceToxic bile acidsModel of cholestasisDuct-ligated animalsExpression of MRP3Intensity of stainingHepatocytes of liverWestern blot analysisBile ductHepatic expressionCentral veinCholestasisMRP3 expressionNormal liverBile acidsPericentral regionsIndirect immunofluorescenceProtein 3Days postligationMRP3Mrp2 proteinLiver
1999
Hepatic Toxicity and Persistence of ser/thr Protein Phosphatase Inhibition by Microcystin in the Little Skate Raja erinacea
Runnegar M, Seward D, Ballatori N, Crawford J, Boyer J. Hepatic Toxicity and Persistence of ser/thr Protein Phosphatase Inhibition by Microcystin in the Little Skate Raja erinacea. Toxicology And Applied Pharmacology 1999, 161: 40-49. PMID: 10558922, DOI: 10.1006/taap.1999.8783.Peer-Reviewed Original ResearchConceptsDose-dependent inhibitionSkate hepatocytesUptake of microcystinNear complete inhibitionPP activityInflammatory changesHepatic toxicityHepatocyte necrosisHistological changesAccompanying inhibitionHepatic lesionsBile acidsHigh dosesOnly organSignificant inhibitionSkate Raja erinaceaLittle skate Raja erinaceaInhibitionLiverRectal gland
1998
Intracellular pH regulation in bombesin-stimulated secretion in isolated bile duct units from rat liver
Cho W, Mennone A, Boyer J. Intracellular pH regulation in bombesin-stimulated secretion in isolated bile duct units from rat liver. American Journal Of Physiology 1998, 275: g1028-g1036. PMID: 9815033, DOI: 10.1152/ajpgi.1998.275.5.g1028.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntiportersBicarbonatesBile Ducts, IntrahepaticBombesinCarrier ProteinsChloride-Bicarbonate AntiportersChloridesFluoresceinsFluorescent DyesHEPESHydrogen-Ion ConcentrationIn Vitro TechniquesMaleModels, BiologicalRatsRats, Sprague-DawleySodiumSodium-Bicarbonate SymportersSodium-Hydrogen ExchangersConceptsHCO-3 secretionBile duct unitsDuct unitsAcid-extruding mechanismsEffect of bombesinRat liverCl-/HCOBombesin-stimulated secretionRegulation of intracellularBiliary secretionNormal rat liverBombesinCholangiocytesSecretionUnderlying mechanismLiverBasalPH regulationCounterregulatoryNeuropeptidesExchange activityActivity
1997
Enterohepatic circulation of scymnol sulfate in an elasmobranch, the little skate (Raja erinacea)
Fricker G, Wössner R, Drewe J, Fricker R, Boyer J. Enterohepatic circulation of scymnol sulfate in an elasmobranch, the little skate (Raja erinacea). American Journal Of Physiology 1997, 273: g1023-g1030. PMID: 9374698, DOI: 10.1152/ajpgi.1997.273.5.g1023.Peer-Reviewed Original ResearchConceptsEnterohepatic circulationScymnol sulfateBile alcohol sulfatesBile flowBiliary secretionCholeretic effectMajor bile saltsHepatic uptakeHepatic eliminationIntestinal epitheliumBileLiverSkate liverHepatic transportSpecific transport mechanismsBile alcoholsMajor metaboliteFirst direct demonstrationBile saltsCholyltaurinePerfusateLittle skatePolar metabolitesCompetitive mannerDirect demonstrationBombesin stimulates bicarbonate secretion from rat cholangiocytes: Implications for neural regulation of bile secretion
Cho W, Mennone A, Rydberg, Boyer J. Bombesin stimulates bicarbonate secretion from rat cholangiocytes: Implications for neural regulation of bile secretion. Gastroenterology 1997, 113: 311-321. PMID: 9207292, DOI: 10.1016/s0016-5085(97)70109-4.Peer-Reviewed Original ResearchConceptsBile secretionBicarbonate secretionRat liverBile duct-ligated ratsRole of bombesinHepatocyte coupletsEffect of bombesinDose-dependent increaseDuct-ligated ratsBile duct unitsCanalicular bile secretionBile pHSite of actionBile flowSubstance PReceptor inhibitorsNeural regulationAbstractTextBombesinBiliary transportDuct unitsSecretionLiverLuminal pHRat cholangiocytes
1995
Isolation of small polarized bile duct units.
Mennone A, Alvaro D, Cho W, Boyer J. Isolation of small polarized bile duct units. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 6527-6531. PMID: 7541542, PMCID: PMC41551, DOI: 10.1073/pnas.92.14.6527.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthine4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsBicarbonatesBile DuctsBucladesineChloridesCholestasisColforsinFluoresceinsGluconatesHydrogen-Ion ConcentrationKineticsLiverMaleMicroscopy, ElectronMicroscopy, VideoRatsRats, Sprague-DawleySecretinTime FactorsConceptsBile duct unitsInterlobular bile ductsBile ductDuct unitsSmall interlobular bile ductsBile duct syndromeBile duct segmentsCholestatic liver disordersCl-/HCO3- exchange activityRat liverLiver disordersPrimary siteForskolin administrationMicroM forskolinDextran 40Duct segmentsDibutyryl cAMPUseful preparationLiverSecretionForskolinImaging techniquesDuctLuminal spaceSyndromeExpression of sodium-independent organic anion uptake systems of skate liver in Xenopus laevis oocytes
Jacquemin E, Hagenbuch B, Wolkoff A, Meier P, Boyer J. Expression of sodium-independent organic anion uptake systems of skate liver in Xenopus laevis oocytes. American Journal Of Physiology 1995, 268: g18-g23. PMID: 7840202, DOI: 10.1152/ajpgi.1995.268.1.g18.Peer-Reviewed Original ResearchConceptsBSP uptakeSkate liverTaurocholate uptakeXenopus laevis oocytesOrganic anion transport systemInjection of oocytesLaevis oocytesLiverAnion transport systemRat liverSulfobromophthalein uptakeUptake systemOocytesMRNADisulfonic acidUptake activityFunctional expressionLiver mRNAUptakeExpressionSerum albuminLower vertebrates
1994
A volume-activated taurine channel in skate hepatocytes: membrane polarity and role of intracellular ATP
Ballatori N, Simmons T, Boyer J. A volume-activated taurine channel in skate hepatocytes: membrane polarity and role of intracellular ATP. American Journal Of Physiology 1994, 267: g285-g291. PMID: 7521136, DOI: 10.1152/ajpgi.1994.267.2.g285.Peer-Reviewed Original ResearchConceptsCell swellingSkate hepatocytesIntracellular ATPExtracellular taurine concentrationMetabolic inhibitorsSinusoidal circulationHypotonic stimuliTaurine concentrationsTaurine effluxTaurine uptakeIndependent uptakeTaurine channelSkate liverEfflux mechanismTaurineLiverHepatocytesInhibitorsEffluxBasolateral membraneOsmolyte taurineSwellingAntimycin ADisulfonic acidTransport inhibitors
1993
Intracellular alkalinization stimulates bile flow and vesicular-mediated exocytosis in IPRL
Bruck R, Benedetti A, Strazzabosco M, Boyer J. Intracellular alkalinization stimulates bile flow and vesicular-mediated exocytosis in IPRL. American Journal Of Physiology 1993, 265: g347-g353. PMID: 8368316, DOI: 10.1152/ajpgi.1993.265.2.g347.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAlkaliesAnimalsAnionsBariumBicarbonatesBileBiological TransportColchicineExocytosisHorseradish PeroxidaseHydrogen-Ion ConcentrationIn Vitro TechniquesIntracellular MembranesLiverMalePotassium ChannelsRatsRats, Sprague-DawleyConceptsBiliary HCO3- excretionBile flowHCO3- excretionIntracellular alkalinizationHCO3- exchangerAcute alkaline loadVesicle-mediated exocytosisBiliary excretionTransient increasePH recoveryAlkaline loadMembrane depolarizationRat liverChannel blocker BaCl2Intact liverLiverAlkalinizationMM NH4ClHCO3- concentrationDisulfonic acidDIDSRat hepatocytesColchicinePretreatmentExocytosis
1992
Papaverine inhibits transcytotic vesicle transport and lipid excretion into bile in isolated perfused rat liver
Hayakawa T, Katagiri K, Hoshino M, Nakai T, Ohiwa T, Kumai T, Miyaji M, Takeuchi T, Corasanti J, Boyer J. Papaverine inhibits transcytotic vesicle transport and lipid excretion into bile in isolated perfused rat liver. Hepatology 1992, 16: 1036-1042. PMID: 1398483, DOI: 10.1002/hep.1840160429.Peer-Reviewed Original ResearchConceptsSodium taurocholate infusionBiliary excretionTaurocholate infusionPhosphodiesterase inhibitorBile acidsNonspecific smooth muscle relaxantSmooth muscle relaxantRat liver modelRat liverLipid excretionContinuous infusionBile flowMuscle relaxantsPapaverine inhibitsConstant infusionHorseradish peroxidaseInfusionPapaverineExcretionInhibitory effectCyclic GMPBileLiverLiver modelCyclic AMPRegulatory volume decrease stimulates bile flow, bile acid excretion, and exocytosis in isolated perfused rat liver
Bruck R, Haddad P, Graf J, Boyer J. Regulatory volume decrease stimulates bile flow, bile acid excretion, and exocytosis in isolated perfused rat liver. American Journal Of Physiology 1992, 262: g806-g812. PMID: 1590390, DOI: 10.1152/ajpgi.1992.262.5.g806.Peer-Reviewed Original ResearchEffects of Ca2+ agonists on cytosolic Ca2+ in isolated hepatocytes and on bile secretion in the isolated perfused rat liver
Nathanson M, Gautam A, Bruck R, Isales C, Boyer J. Effects of Ca2+ agonists on cytosolic Ca2+ in isolated hepatocytes and on bile secretion in the isolated perfused rat liver. Hepatology 1992, 15: 107-116. PMID: 1727785, DOI: 10.1002/hep.1840150119.Peer-Reviewed Original ResearchConceptsBile flowPerfusion pressureBile secretionCytosolic Ca2Rat liverProtein kinase CIonophore-induced increaseBromo-A23187Hepatic vasoconstrictionVasodilator papaverineProtein kinase C inhibitor HExtracellular Ca2Kinase CSecretionLiverVasopressinAgonist vasopressinBenzohydroquinoneEffect of Ca2HepatocytesActivate protein kinase CInhibitor HTwo-thirdsIsolated hepatocytesSensitive photoprotein aequorin
1991
Role of chloride ions in liver cell volume regulation
Haddad P, Beck J, Boyer J, Graf J. Role of chloride ions in liver cell volume regulation. American Journal Of Physiology 1991, 261: g340-g348. PMID: 1872402, DOI: 10.1152/ajpgi.1991.261.2.g340.Peer-Reviewed Original ResearchConceptsRegulatory volume decreaseM omegaLiver cellsSingle isolated rat hepatocytesBiphasic responseHypotonic stressInitial hyperpolarizationPl/minIsolated rat hepatocytesGradual depolarizationCell volume regulationExternal ClRole of C1Hypotonic swellingVolume regulationMembrane potentialFacilitated releaseRat hepatocytesCellsMinVolume decreaseCell volumeInitial volumeInitial rateLiverEffect of UDCA on intracellular and biliary pH in isolated rat hepatocyte couplets and perfused livers
Strazzabosco M, Sakisaka S, Hayakawa T, Boyer J. Effect of UDCA on intracellular and biliary pH in isolated rat hepatocyte couplets and perfused livers. American Journal Of Physiology 1991, 260: g58-g69. PMID: 1987808, DOI: 10.1152/ajpgi.1991.260.1.g58.Peer-Reviewed Original ResearchConceptsBiliary HCO3- concentrationUrsodeoxycholic acidRat hepatocyte coupletsBiliary pHAcid loadEffect of UDCABile duct lumenHepatocyte coupletsIsolated rat hepatocyte coupletsBiliary epitheliumHCO3- concentrationDuct lumenRemoval of ClHCO3- exchangeRat liverLiverIPRLHepatocytesHypercholeresisPerfusateBileExcretionEpitheliumAmiloride
1990
DBcAMP stimulates vesicle transport and HRP excretion in isolated perfused rat liver
Hayakawa T, Bruck R, Ng O, Boyer J. DBcAMP stimulates vesicle transport and HRP excretion in isolated perfused rat liver. American Journal Of Physiology 1990, 259: g727-g735. PMID: 2173414, DOI: 10.1152/ajpgi.1990.259.5.g727.Peer-Reviewed Original ResearchConceptsBiliary excretionBile acidsBile acid excretionLiver 1Effects of adenosineRat liverElectron microscopic morphometric analysisTranscytotic vesicle pathwayLate peakHRP excretionHorseradish peroxidaseMin infusionBile flowExcretory functionAcid excretionO-dibutyryl cAMPPrimary hepatocyte culturesExcretionLiverDbcAMPCyclic monophosphateInfusionPericanalicular area
1989
Voltage-driven, taurocholate-dependent secretion in isolated hepatocyte couplets
Weinman S, Graf J, Boyer J. Voltage-driven, taurocholate-dependent secretion in isolated hepatocyte couplets. American Journal Of Physiology 1989, 256: g826-g832. PMID: 2719107, DOI: 10.1152/ajpgi.1989.256.5.g826.Peer-Reviewed Original ResearchConceptsBile secretionBile acidsSecretion rateFluid secretionIntracellular current injectionCanalicular areaHepatocyte coupletsMembrane potentialRat hepatocyte coupletsAbsence of taurocholateMicroM taurocholateFluid secretion rateBile formationSecretionCarrier-mediated transportTaurocholateLiverIntracellular voltageDeterminants of glutathione efflux and biliary GSH/GSSG ratio in perfused rat liver
Ballatori N, Truong A, K. A, Boyer J. Determinants of glutathione efflux and biliary GSH/GSSG ratio in perfused rat liver. American Journal Of Physiology 1989, 256: g482-g490. PMID: 2564253, DOI: 10.1152/ajpgi.1989.256.3.g482.Peer-Reviewed Original ResearchConceptsGamma-glutamyl transferase activityO2 deliveryBiliary glutathioneHepatic effluxSinusoidal effluxKrebs-Ringer bicarbonate bufferGlutathione effluxRat liverEfflux of glutathioneGSH/GSSG ratioRed blood cellsPerfusion flow rateFluorocarbon emulsionsBile flowTissue GSHOxygen deliveryTransferase activityAT-125LiverBovine red blood cellsPerfusate compositionBlood cellsBileGSSG levelsGSSG ratio