2019
Patient-Derived Organoids from Human Bile: An In Vitro Method to Study Cholangiopathies
Soroka CJ, Assis DN, Boyer JL. Patient-Derived Organoids from Human Bile: An In Vitro Method to Study Cholangiopathies. Methods In Molecular Biology 2019, 1981: 363-372. PMID: 31016667, DOI: 10.1007/978-1-4939-9420-5_24.BooksConceptsPrimary sclerosing cholangitisPrimary sclerosing cholangitis patientsEndoscopic retrograde cholangiopancreatographySclerosing cholangitisCholangitis patientsRetrograde cholangiopancreatographyExtrahepatic bile duct epitheliumTherapeutic endoscopic retrograde cholangiopancreatographyChemo/cytokinesEnd-stage diseaseBile duct epitheliumPatient-derived organoidsPharmacotherapeutic treatmentClinical indicationsIndividual patientsInflammatory stimuliHeterogeneous diseaseBiliary phenotypeDuct epitheliumDisease statusCholangitisCholangiopathyHuman bileBiliary cellsPatients
2001
Cl−-dependent secretory mechanisms in isolated rat bile duct epithelial units
Singh S, Mennone A, Gigliozzi A, Fraioli F, Boyer J. Cl−-dependent secretory mechanisms in isolated rat bile duct epithelial units. AJP Gastrointestinal And Liver Physiology 2001, 281: g438-g446. PMID: 11447024, DOI: 10.1152/ajpgi.2001.281.2.g438.Peer-Reviewed Original ResearchMeSH KeywordsAmmonium ChlorideAnimalsBariumBicarbonatesBile Ducts, IntrahepaticBumetanideCarbon DioxideCarrier ProteinsCell PolarityCells, CulturedChloridesColforsinEpithelial CellsHydrogen-Ion ConcentrationIon TransportMaleModels, BiologicalRatsRats, Sprague-DawleySodiumSodium-Potassium-Chloride SymportersConceptsCO2/HCOFluid secretionCotransport activityIntrahepatic bile duct epitheliumBile duct epitheliumDependent fluid secretionHigh intracellular Cl(-) concentrationIntracellular Cl- concentrationBile duct unitsApical Cl(-) secretionNH entryInitial acidification rateIntracellular cAMP concentrationNH4Cl exposureDuct epitheliumInhibitor bumetanideChannel inhibitorsCl(-) secretionCanalicular bileDuct unitsSecretionDependent secretionSecretory mechanismBumetanideForskolinIsolation of functional polarized bile duct units from mouse liver
Cho W, Mennone A, Boyer J. Isolation of functional polarized bile duct units from mouse liver. AJP Gastrointestinal And Liver Physiology 2001, 280: g241-g246. PMID: 11208546, DOI: 10.1152/ajpgi.2001.280.2.g241.Peer-Reviewed Original ResearchConceptsVasoactive intestinal peptideBile duct epitheliumMurine animal modelBile duct unitsPortal perfusionIntestinal peptideSecretory responseAnimal modelsBlunt dissectionDuct epitheliumSecretory stimuliFluid secretionDuct unitsCholangiocyte secretionFurther enzymatic digestionMouse liverSecretionMurine animalsIBDUMice
1996
Bile duct epithelium: frontiers in transport physiology
Boyer J. Bile duct epithelium: frontiers in transport physiology. American Journal Of Physiology 1996, 270: g1-g5. PMID: 8772494, DOI: 10.1152/ajpgi.1996.270.1.g1.Peer-Reviewed Original Research