2015
Characterization of a Small‐molecule Modulator of IRE1α Activity
Salami‐Oyenuga J, Raina K, Crews C. Characterization of a Small‐molecule Modulator of IRE1α Activity. The FASEB Journal 2015, 29 DOI: 10.1096/fasebj.29.1_supplement.723.2.Peer-Reviewed Original ResearchUnfolded protein responseHuman IRE1αIRE1α activityInositol-Requiring Enzyme 1X-box binding protein 1 (XBP1) mRNAER stress conditionsEnzymatic activitySmall molecule modulatorsMechanism of regulationER stress responseHEK293T cellsThermal shift assayER stress pathwayEndoplasmic reticulum stressCellular processesER lumenUPR signalingShift assaysIRE1α activationProtein responseHuman kidney cellsIRE1α kinaseStress responseIRE1α proteinCentral player
2014
Small‐Molecule Control of Intracellular Protein Levels through Modulation of the Ubiquitin Proteasome System
Buckley DL, Crews CM. Small‐Molecule Control of Intracellular Protein Levels through Modulation of the Ubiquitin Proteasome System. Angewandte Chemie International Edition 2014, 53: 2312-2330. PMID: 24459094, PMCID: PMC4348030, DOI: 10.1002/anie.201307761.Peer-Reviewed Original ResearchConceptsSmall molecule modulatorsProtein levelsSmall-molecule probesUbiquitin-proteasome systemActivity of proteinsIntracellular protein levelsBiological probesProteasome systemProtein degradationUbiquitin-proteasomeProtein activitySmall moleculesMolecule controlDruggable targetsProteomeProteasomeTargeted fashionProteinRemaining majorityGlobal increaseProbeUPSMoleculesDegradationMultiple strategies