2024
Competition and synergy of Arp2/3 and formins in nucleating actin waves
Le Chua X, San Tong C, Su M, Xǔ X, Xiao S, Wu X, Wu M. Competition and synergy of Arp2/3 and formins in nucleating actin waves. Cell Reports 2024, 43: 114423. PMID: 38968072, PMCID: PMC11378572, DOI: 10.1016/j.celrep.2024.114423.Peer-Reviewed Original ResearchActin wavesActin regulatory proteinsActin cytoskeletal networkConstitutively active mutantArp2/3 complexActin assemblyMonomeric actinActive Cdc42Cdc42-interactingCytoskeletal networkArp2/3Active mutantCellular processesRegulatory proteinsUpstream regulatorCdc42ActinPlasma membraneFMNL1Complex feedback loopsForminPhysiological stateDelayed recruitmentFeedback loopGTPase
2018
Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System
Yong J, Chen Y, Wu M. Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System. Methods In Molecular Biology 2018, 1847: 177-187. PMID: 30129017, DOI: 10.1007/978-1-4939-8719-1_13.Peer-Reviewed Original ResearchExtracellular anti-angiogenic proteins augment an endosomal protein trafficking pathway to reach mitochondria and execute apoptosis in HUVECs
Chen M, Qiu T, Wu J, Yang Y, Wright GD, Wu M, Ge R. Extracellular anti-angiogenic proteins augment an endosomal protein trafficking pathway to reach mitochondria and execute apoptosis in HUVECs. Cell Death & Differentiation 2018, 25: 1905-1920. PMID: 29523874, PMCID: PMC6219483, DOI: 10.1038/s41418-018-0092-9.Peer-Reviewed Original ResearchMeSH KeywordsAngiostatinsApoptosisCell MembraneEndocytosisEndoplasmic Reticulum Chaperone BiPEndosomesFibronectinsFluorescence Recovery After PhotobleachingHeat-Shock ProteinsHuman Umbilical Vein Endothelial CellsHumansMicroscopy, FluorescenceMitochondriaNeovascularization, PhysiologicPhosphoproteinsProtein TransportRecombinant ProteinsRNA InterferenceRNA, Small InterferingSodium-Hydrogen ExchangersSynaptosomal-Associated Protein 25ThrombospondinsConceptsLate endosomesEndosomal proteinExtracellular proteinsT-SNARE proteinsCytosolic scaffold proteinsTotal internal reflection fluorescent microscopyPro-apoptotic functionFluorescent microscopyRegulatory factor 1Recycling endosomesRNAi knockdownCargo degradationScaffold proteinSuper-resolution fluorescent microscopyMitochondrial traffickingCell fractionationPlasma membraneIsthminProximity ligationMitochondriaProtein 25ProteinBiochemical analysisAnti-angiogenic proteinFactor 1
2017
Clathrin Assembly Defines the Onset and Geometry of Cortical Patterning
Yang Y, Xiong D, Pipathsouk A, Weiner OD, Wu M. Clathrin Assembly Defines the Onset and Geometry of Cortical Patterning. Developmental Cell 2017, 43: 507-521.e4. PMID: 29161594, PMCID: PMC5826602, DOI: 10.1016/j.devcel.2017.10.028.Peer-Reviewed Original ResearchConceptsPlasma membraneClathrin assemblyClathrin functionEndocytic proteinsMembrane traffickingEndocytic machineryN-WASPEndocytic eventsSignal transductionDownstream factorsCell surfaceCortical patterningPattern formationAssemblyMembraneImportant insightsActive processSingle-cell patternFBP17Cdc42ClathrinEndocytosisTransductionCortical pattern formationPhosphatidylinositol
2013
Calcium oscillations-coupled conversion of actin travelling waves to standing oscillations
Wu M, Wu X, De Camilli P. Calcium oscillations-coupled conversion of actin travelling waves to standing oscillations. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 1339-1344. PMID: 23297209, PMCID: PMC3557052, DOI: 10.1073/pnas.1221538110.Peer-Reviewed Original ResearchConceptsPlasma membraneActin regulatory proteinsActin cytoskeletonActin dynamicsActive Cdc42Cell cortexCell physiologyRegulatory proteinsActin polymerizationDynamic spatial patternsMacromolecular assembliesCalcium oscillationsSpatial patternsActinMembraneFBP17Cdc42CellsCytoskeletonFeedback mechanismProteinPhysiologyAntigen stimulationGlobal fluctuationsMechanism
2012
Chapter 1 Supported Native Plasma Membranes as Platforms for the Reconstitution and Visualization of Endocytic Membrane Budding
Wu M, De Camilli P. Chapter 1 Supported Native Plasma Membranes as Platforms for the Reconstitution and Visualization of Endocytic Membrane Budding. Methods In Cell Biology 2012, 108: 1-18. PMID: 22325595, DOI: 10.1016/b978-0-12-386487-1.00001-8.Peer-Reviewed Original ResearchConceptsPlasma membranePlasma membrane sheetsNative plasma membraneEndocytic budsCell-free assayMembrane buddingEndocytic eventsMolecular dissectionElucidation of mechanismsCytosolic extractsBiological membranesMembrane sheetsEndocytosisExtracellular materialRobust formationMembraneSubsequent incubationVariety of manipulationsReconstitutionCellsExperimental systemPowerful toolFissionBuddingBuds