2021
Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice
Xu S, Li J, Yang L, Wang CJ, Liu T, Weinstein AM, Palmer LG, Wang T. Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice. Pflügers Archiv - European Journal Of Physiology 2021, 473: 1749-1760. PMID: 34455480, PMCID: PMC8528772, DOI: 10.1007/s00424-021-02611-5.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateFemale miceUrine volumeNaCl cotransporterLow K intakeMeasurement of functionSex differencesNatriuretic responseFractional excretionK intakeTransport protein expressionFiltration rateNCC abundanceNCC expressionRenal clearanceDistal nephronLK dietCation excretionPlasma KNCC proteinNKCC2 expressionWestern blottingProtein expressionMiceFENa
2019
Sex difference in kidney electrolyte transport II: impact of K+ intake on thiazide-sensitive cation excretion in male and female mice
Li J, Xu S, Yang L, Yang J, Wang CJ, Weinstein AM, Palmer LG, Wang T. Sex difference in kidney electrolyte transport II: impact of K+ intake on thiazide-sensitive cation excretion in male and female mice. American Journal Of Physiology. Renal Physiology 2019, 317: f967-f977. PMID: 31390232, PMCID: PMC6843050, DOI: 10.1152/ajprenal.00125.2019.Peer-Reviewed Original ResearchConceptsHK dietCation excretionWT animalsFemale animalsMale animalsSex differencesRenal clearance experimentsGlomerular filtration rateR KO miceExchanger isoform 3Distal NaHK intakeWestern blot analysisFractional excretionUrine volumeFiltration rateFemale miceKO miceNHE3 abundanceClearance experimentsNCC abundanceLittle sex differenceKnockout miceENaC expressionExcretion
2018
Potassium conservation is impaired in mice with reduced renal expression of Kir4.1
Malik S, Lambert E, Zhang J, Wang T, Clark H, Cypress M, Goldman B, Porter GA, Pena S, Nino W, Gray D. Potassium conservation is impaired in mice with reduced renal expression of Kir4.1. American Journal Of Physiology. Renal Physiology 2018, 315: f1271-f1282. PMID: 30110571, PMCID: PMC6293297, DOI: 10.1152/ajprenal.00022.2018.Peer-Reviewed Original ResearchMeSH KeywordsAlkalosisAnimalsAquaporin 3Gene Knockdown TechniquesGenotypeHypercalcemiaHyperkalemiaHypernatremiaKidney Concentrating AbilityMice, Inbred C57BLMice, KnockoutNephronsPhenotypePhosphorylationPotassium Channels, Inwardly RectifyingPotassium, DietaryRenal ReabsorptionSolute Carrier Family 12, Member 3ConceptsMg-free dietSevere urinary concentrating defectReduced renal expressionRenal K wastingSeSAME/EAST syndromeDistal convoluted tubuleKir4.1 protein expressionWild-type miceUrinary concentrating defectCre-LoxP methodologyAquaporin 3 expressionRelative hypercalcemiaRenal expressionPotassium conservationMetabolic alkalosisNCC expressionChannel Kir4.1Distal nephronKnockout miceConvoluted tubulesCotransporter expressionEAST syndromeHypokalemiaMiceReduced expression
2015
Regulation of glomerulotubular balance. III. Implication of cytosolic calcium in flow-dependent proximal tubule transport
Du Z, Weinbaum S, Weinstein A, Wang T. Regulation of glomerulotubular balance. III. Implication of cytosolic calcium in flow-dependent proximal tubule transport. American Journal Of Physiology. Renal Physiology 2015, 308: f839-f847. PMID: 25651568, PMCID: PMC4398834, DOI: 10.1152/ajprenal.00601.2014.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBicarbonatesBiological TransportCalciumCalcium-Transporting ATPasesChelating AgentsCytosolEnzyme InhibitorsIn Vitro TechniquesInositol 1,4,5-Trisphosphate ReceptorsKidney Tubules, ProximalKineticsMice, Inbred C57BLPerfusionRenal ReabsorptionSodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsNa/H exchanger 3Luminal Ca(2Flow-induced increaseCytosolic calciumProximal tubulesCa(2+) chelator BAPTA-AMAntagonist 2-aminoethoxydiphenyl borateCa-ATPase inhibitor thapsigarginProximal tubule transportNa(+) reabsorptionMicroperfused in vitroMouse proximal tubuleChelator BAPTA-AMH-ATPase activityNo effectControl tubulesInhibitor thapsigarginBAPTA-AMIntracellular Ca(2Decreased transportTubule transportBaseline transportStimulates Na(+Modulate Na(+Glomerulotubular balance
2014
Kir1.1 (ROMK) and Kv7.1 (KCNQ1/KvLQT1) are essential for normal gastric acid secretion: importance of functional Kir1.1
Vucic E, Alfadda T, MacGregor GG, Dong K, Wang T, Geibel JP. Kir1.1 (ROMK) and Kv7.1 (KCNQ1/KvLQT1) are essential for normal gastric acid secretion: importance of functional Kir1.1. Pflügers Archiv - European Journal Of Physiology 2014, 467: 1457-1468. PMID: 25127675, DOI: 10.1007/s00424-014-1593-0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsGastric AcidGastric MucosaKCNQ1 Potassium ChannelMiceMice, Inbred C57BLPotassium Channels, Inwardly RectifyingStomachXenopusConceptsGastric parietal cellsPotassium channelsParietal cellsΒ-subunitKir1.1 channelsWild-type miceSecretagogue-stimulated gastric acid secretionApical poleGastric glandsLeak pathwayPotential therapeutic targetKir1.1Proton secretionRegulatory characteristicsKv7.1Therapeutic targetATPaseCell numberParietal cell numberCellsNormal gastric acid secretionSecretionInhibitorsAcid secretionMice
2013
Proximal tubule specific knockout of the Na+/H+ exchanger NHE3: effects on bicarbonate absorption and ammonium excretion
Li H, Du Z, Barone S, Rubera I, McDonough A, Tauc M, Zahedi K, Wang T, Soleimani M. Proximal tubule specific knockout of the Na+/H+ exchanger NHE3: effects on bicarbonate absorption and ammonium excretion. Journal Of Molecular Medicine 2013, 91: 951-963. PMID: 23508938, PMCID: PMC3730089, DOI: 10.1007/s00109-013-1015-3.Peer-Reviewed Original ResearchConceptsNa+/H+ exchanger NHE3Proximal convoluted tubulesKO miceMetabolic acidosisBicarbonate reabsorptionDays of acid loadingIsolated proximal convoluted tubulesConvoluted tubulesIn vitro microperfusion studyCompared to WT miceNHE3 knockout miceProximal tubule NHE3Acid loadNH4Cl acid loadMild metabolic acidosisWT miceNHE3Microperfusion studiesKnockout miceBicarbonate absorptionMutant miceVolume reabsorptionFloxed miceNH4Cl loadingTransgenic mice
2010
Identification and Regulation of Reticulon 4B (Nogo-B) in Renal Tubular Epithelial Cells
Marin EP, Moeckel G, Al-Lamki R, Bradley J, Yan Q, Wang T, Wright PL, Yu J, Sessa WC. Identification and Regulation of Reticulon 4B (Nogo-B) in Renal Tubular Epithelial Cells. American Journal Of Pathology 2010, 177: 2765-2773. PMID: 20971739, PMCID: PMC2993268, DOI: 10.2353/ajpath.2010.100199.Peer-Reviewed Original ResearchConceptsUnilateral ureteral obstructionAcute tubular necrosisEpithelial cellsRenal tubular epithelial cellsMurine kidneyIschemia/reperfusionMeasurement of fibrosisDistal nephron segmentsRecruitment of macrophagesWild-type miceInflammatory gene expressionTubular epithelial cellsDe novo expressionHuman biopsy specimensRenal injuryTubular necrosisUreteral obstructionWT miceVascular injuryHistological damageBiopsy specimensCortical tubulesDeficient miceMacrophage recruitmentTissue injury
2009
Inward rectifier channel, ROMK, is localized to the apical tips of glial‐like cells in mouse taste buds
Dvoryanchikov G, Sinclair M, Perea‐Martinez I, Wang T, Chaudhari N. Inward rectifier channel, ROMK, is localized to the apical tips of glial‐like cells in mouse taste buds. The Journal Of Comparative Neurology 2009, 517: 1-14. PMID: 19708028, PMCID: PMC3104395, DOI: 10.1002/cne.22152.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsFluorescent Antibody TechniqueGap JunctionsGlutamate DecarboxylaseGreen Fluorescent ProteinsImmunohistochemistryKidneyMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicNeurogliaPhospholipase C betaPotassium Channels, Inwardly RectifyingProtein IsoformsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTaste BudsTight JunctionsConceptsTaste budsTaste cellsTight junctionsHyperpolarized resting membrane potentialInwardly rectifying K channelsMouse taste budsRT-PCRApical tight junctionsMouse taste cellsInward rectifier channelsFungiform taste budsReverse-transcription polymerase chain reactionGlial cell markersQuantitative (q)RT-PCRGlial-like cellsROMK mRNAApical tipExtracellular K(+Rectifier channelsCell markersPolymerase chain reactionK channelsTransgenic miceAction potentialsBuds
2005
Axial flow modulates proximal tubule NHE3 and H-ATPase activities by changing microvillus bending moments
Du Z, Yan Q, Duan Y, Weinbaum S, Weinstein A, Wang T. Axial flow modulates proximal tubule NHE3 and H-ATPase activities by changing microvillus bending moments. American Journal Of Physiology. Renal Physiology 2005, 290: f289-f296. PMID: 16144961, DOI: 10.1152/ajprenal.00255.2005.Peer-Reviewed Original ResearchConceptsProximal tubules in vitroHCO3- absorptionTubules in vitroBrush-border microvilliHCO3- permeabilityH(+)-ATPase inhibitor bafilomycinInhibition of NHE3Proximal tubule NHE3Transepithelial potential differenceH-ATPase activityLuminal cell membraneH(+)-ATPaseTransepithelial PDNa+ absorptionParacellular permeabilityH(+)-ATPase activityInhibitor EIPAMembrane transport activityProximal tubulesHCO3- reabsorptionNHE3HCO3- transportH-ATPaseBicarbonate transportPermeability changes
2004
Mechanosensory function of microvilli of the kidney proximal tubule
Du Z, Duan Y, Yan Q, Weinstein A, Weinbaum S, Wang T. Mechanosensory function of microvilli of the kidney proximal tubule. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 13068-13073. PMID: 15319475, PMCID: PMC516518, DOI: 10.1073/pnas.0405179101.Peer-Reviewed Original ResearchConceptsKidney proximal tubulesProximal tubulesNa+ reabsorptionNa+ transportNa+-H+ exchange activityProximal tubule Na+ reabsorptionNa+-H+ exchangeLuminal cell membraneMechanosensory functionNa+ absorptionTubular diameterLuminal diameterMouse kidneyEpithelial microvilliReabsorptionTubulesKidneyAutoregulatory mechanismNa+ uptakeHormonal systemsMiceActin cytoskeletonCell membraneTerminal webBending moment
2000
Defective fluid and HCO3 − absorption in proximal tubule of neuronal nitric oxide synthase-knockout mice
Wang T, Inglis F, Kalb R. Defective fluid and HCO3 − absorption in proximal tubule of neuronal nitric oxide synthase-knockout mice. American Journal Of Physiology. Renal Physiology 2000, 279: f518-f524. PMID: 10966931, DOI: 10.1152/ajprenal.2000.279.3.f518.Peer-Reviewed Original ResearchMeSH KeywordsAcid-Base EquilibriumAnimalsBicarbonatesBiological TransportBlood Gas AnalysisBody FluidsElectrolytesEnzyme InhibitorsInjections, IntravenousKidney Tubules, ProximalMiceMice, Inbred C57BLMice, KnockoutNG-Nitroarginine Methyl EsterNitric Oxide SynthaseNitric Oxide Synthase Type IPotassiumSodiumConceptsNNOS knockout miceWild-type miceL-NAMEProximal tubulesMetabolic acidosisAbsorption of fluidDecreased absorption of fluidBlood pressureIncreased mean blood pressureResponse to L-NAMEWild-type control animalsFluid absorptionAdministration of L-NAMEIn situ microperfusionProximal tubule transportNeuronal nitric oxide synthaseLuminal perfusion solutionMean blood pressureRenal clearance techniquesNitric oxide synthaseRates of HCO(3)(-Urinary Na(+HCO3- absorptionRegulation of acid-base balanceTubule transport
1998
A tyrosine-based signal regulates H-K-ATPase-mediated potassium reabsorption in the kidney
Wang T, Courtois-Coutry N, Giebisch G, Caplan M. A tyrosine-based signal regulates H-K-ATPase-mediated potassium reabsorption in the kidney. American Journal Of Physiology 1998, 275: f818-f826. PMID: 9815140, DOI: 10.1152/ajprenal.1998.275.5.f818.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsH(+)-K(+)-Exchanging ATPaseKidney Tubules, CollectingMaleMiceMice, Inbred C57BLMice, TransgenicPotassiumSignal TransductionTyrosineConceptsGlomerular filtration rateTransgenic miceGastric acid outputPlasma K concentrationK pumpK-ATPaseRenal collecting tubulesK clearanceBlood pressurePotassium reabsorptionAcid outputUrine volumeK excretionFiltration rateGastric acidK reabsorptionPump functionCollecting tubuleMicePlasma NaTyrosine-based sequenceTyrosine-based signalsKidneyExcretionCytoplasmic tail