Preactivation of AMPK by metformin may ameliorate the epithelial cell damage caused by renal ischemia
Seo-Mayer PW, Thulin G, Zhang L, Alves DS, Ardito T, Kashgarian M, Caplan MJ. Preactivation of AMPK by metformin may ameliorate the epithelial cell damage caused by renal ischemia. American Journal Of Physiology. Renal Physiology 2011, 301: f1346-f1357. PMID: 21849490, PMCID: PMC3233870, DOI: 10.1152/ajprenal.00420.2010.Peer-Reviewed Original ResearchConceptsEpithelial cell polarityMDCK cellsPlasma membrane domainsIon transport proteinsEpithelial cell organizationCellular energy sensorAMPK activator metforminMadin-Darby canine kidney cellsBasolateral plasma membraneShort hairpin RNACanine kidney cellsCell polarityImmunofluoresence localizationRenal epithelial cellsMembrane domainsNa-K-ATPaseProtein kinaseAMPK activatorPlasma membraneVesicular compartmentsAMPK activityTransport proteinsEnergy sensorMolecular consequencesBasolateral localization