Stefan Krimmer
Associate Research Scientist in PharmacologyCards
About
Research
Publications
Featured Publications
Cryo-EM analyses of KIT and oncogenic mutants reveal structural oncogenic plasticity and a target for therapeutic intervention
Krimmer S, Bertoletti N, Suzuki Y, Katic L, Mohanty J, Shu S, Lee S, Lax I, Mi W, Schlessinger J. Cryo-EM analyses of KIT and oncogenic mutants reveal structural oncogenic plasticity and a target for therapeutic intervention. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2300054120. PMID: 36943885, PMCID: PMC10068818, DOI: 10.1073/pnas.2300054120.Peer-Reviewed Original ResearchConceptsOncogenic KIT mutantsStem cell factorKIT mutantsHomotypic contactsCryo-EM analysisUnexpected structural plasticityLigand stem cell factorElectron microscopy structural analysisReceptor tyrosine kinase KITOncogenic mutantsHematopoietic stem cellsKIT dimerizationTyrosine kinase KITD5 regionPlasma membraneMutational analysisMutantsExtracellular domainGerm cellsHuman cancersSomatic gainCell factorStructural plasticityStem cellsKinase KIT
2022
Unveiling molecular insights into the mechanism of activation of oncogenic phosphoinositide 3-kinase mutants
Krimmer S, Schlessinger J. Unveiling molecular insights into the mechanism of activation of oncogenic phosphoinositide 3-kinase mutants. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2218237119. PMID: 36475945, PMCID: PMC9897451, DOI: 10.1073/pnas.2218237119.Peer-Reviewed Original ResearchInsights on JAK2 Modulation by Potent, Selective, and Cell-Permeable Pseudokinase-Domain Ligands
Liosi ME, Ippolito JA, Henry SP, Krimmer SG, Newton AS, Cutrona KJ, Olivarez RA, Mohanty J, Schlessinger J, Jorgensen WL. Insights on JAK2 Modulation by Potent, Selective, and Cell-Permeable Pseudokinase-Domain Ligands. Journal Of Medicinal Chemistry 2022, 65: 8380-8400. PMID: 35653642, PMCID: PMC9939005, DOI: 10.1021/acs.jmedchem.2c00283.Peer-Reviewed Original ResearchConversion of a False Virtual Screen Hit into Selective JAK2 JH2 Domain Binders Using Convergent Design Strategies
Henry SP, Liosi ME, Ippolito JA, Cutrona KJ, Krimmer SG, Newton AS, Schlessinger J, Jorgensen WL. Conversion of a False Virtual Screen Hit into Selective JAK2 JH2 Domain Binders Using Convergent Design Strategies. ACS Medicinal Chemistry Letters 2022, 13: 819-826. PMID: 35586418, PMCID: PMC9109162, DOI: 10.1021/acsmedchemlett.2c00051.Peer-Reviewed Original Research
2021
Structural basis for ligand reception by anaplastic lymphoma kinase
Li T, Stayrook SE, Tsutsui Y, Zhang J, Wang Y, Li H, Proffitt A, Krimmer SG, Ahmed M, Belliveau O, Walker IX, Mudumbi KC, Suzuki Y, Lax I, Alvarado D, Lemmon MA, Schlessinger J, Klein DE. Structural basis for ligand reception by anaplastic lymphoma kinase. Nature 2021, 600: 148-152. PMID: 34819665, PMCID: PMC8639777, DOI: 10.1038/s41586-021-04141-7.Peer-Reviewed Original ResearchIndoloxytriazines as binding molecules for the JAK2 JH2 pseudokinase domain and its V617F variant
Newton AS, Liosi ME, Henry SP, Deiana L, Faver JC, Krimmer SG, Puleo DE, Schlessinger J, Jorgensen WL. Indoloxytriazines as binding molecules for the JAK2 JH2 pseudokinase domain and its V617F variant. Tetrahedron Letters 2021, 77: 153248. PMID: 34393283, PMCID: PMC8357305, DOI: 10.1016/j.tetlet.2021.153248.Peer-Reviewed Original ResearchEngineering diaminotriazole ligands into ATP-mimetics for selective targeting of the Janus kinase 2 (JAK2) pseudokinase domain (JH2)
Liosi M, Krimmer S, Sofia Newton A, Dawson T, Puleo D, Cutrona K, Suzuki Y, Henry S, Schlessinger J, Jorgensen W. Engineering diaminotriazole ligands into ATP-mimetics for selective targeting of the Janus kinase 2 (JAK2) pseudokinase domain (JH2). 2021 DOI: 10.1021/scimeetings.1c00737.Peer-Reviewed Original Research
2020
Metadynamics as a Postprocessing Method for Virtual Screening with Application to the Pseudokinase Domain of JAK2
Cutrona KJ, Newton AS, Krimmer SG, Tirado-Rives J, Jorgensen WL. Metadynamics as a Postprocessing Method for Virtual Screening with Application to the Pseudokinase Domain of JAK2. Journal Of Chemical Information And Modeling 2020, 60: 4403-4415. PMID: 32383599, PMCID: PMC7927942, DOI: 10.1021/acs.jcim.0c00276.Peer-Reviewed Original ResearchSelective Janus Kinase 2 (JAK2) Pseudokinase Ligands with a Diaminotriazole Core
Liosi ME, Krimmer SG, Newton AS, Dawson T, Puleo DE, Cutrona KJ, Suzuki Y, Schlessinger J, Jorgensen WL. Selective Janus Kinase 2 (JAK2) Pseudokinase Ligands with a Diaminotriazole Core. Journal Of Medicinal Chemistry 2020, 63: 5324-5340. PMID: 32329617, PMCID: PMC7949251, DOI: 10.1021/acs.jmedchem.0c00192.Peer-Reviewed Original Research
2018
Bayesian analysis of isothermal titration calorimetry for binding thermodynamics
Nguyen T, Rustenburg A, Krimmer S, Zhang H, Clark J, Novick P, Branson K, Pande V, Chodera J, Minh D. Bayesian analysis of isothermal titration calorimetry for binding thermodynamics. PLOS ONE 2018, 13: e0203224. PMID: 30212471, PMCID: PMC6136728, DOI: 10.1371/journal.pone.0203224.Peer-Reviewed Original Research