2020
Shelterin and the replisome: at the intersection of telomere repair and replication
Cicconi A, Chang S. Shelterin and the replisome: at the intersection of telomere repair and replication. Current Opinion In Genetics & Development 2020, 60: 77-84. PMID: 32171974, DOI: 10.1016/j.gde.2020.02.016.Peer-Reviewed Original Research
2013
SLX4 Assembles a Telomere Maintenance Toolkit by Bridging Multiple Endonucleases with Telomeres
Wan B, Yin J, Horvath K, Sarkar J, Chen Y, Wu J, Wan K, Lu J, Gu P, Yu EY, Lue NF, Chang S, Liu Y, Lei M. SLX4 Assembles a Telomere Maintenance Toolkit by Bridging Multiple Endonucleases with Telomeres. Cell Reports 2013, 4: 861-869. PMID: 24012755, PMCID: PMC4334113, DOI: 10.1016/j.celrep.2013.08.017.Peer-Reviewed Original ResearchConceptsTRFH domainRecombination-based telomere maintenanceTelomeric protein TRF2Nucleolytic resolutionTelomeric localizationMultiple endonucleasesPeptide-binding siteDNA metabolismProtein TRF2Telomere maintenanceSLX4TRF2Molecular mechanismsDNA structureHuman cellsTelomeresSLX1Protein levelsEndonucleaseMotifMus81ComplexesNucleaseXPFDomain
2012
CTC1 deletion results in defective telomere replication, leading to catastrophic telomere loss and stem cell exhaustion
Gu P, Min J, Wang Y, Huang C, Peng T, Chai W, Chang S. CTC1 deletion results in defective telomere replication, leading to catastrophic telomere loss and stem cell exhaustion. The EMBO Journal 2012, 31: 2309-2321. PMID: 22531781, PMCID: PMC3364752, DOI: 10.1038/emboj.2012.96.Peer-Reviewed Original ResearchConceptsMammalian CSTTelomere lossDefective telomere replicationDeletion resultsG2/M checkpointComplete bone marrow failureStem cell exhaustionTelomere deprotectionGenome stabilityTEN1 (CST) complexTelomere replicationReplication forksTelomere maintenanceLength maintenanceCTC1-STN1Efficient restartM checkpointVivo functionCTC1TelomeresAcute deletionBone marrow failureProliferative defectEfficient replicationEssential roleRPA and POT1
Flynn RL, Chang S, Zou L. RPA and POT1. Cell Cycle 2012, 11: 652-657. PMID: 22373525, PMCID: PMC3318101, DOI: 10.4161/cc.11.4.19061.Peer-Reviewed Original ResearchConceptsReplication protein ATelomere maintenanceDNA replicationProtein complex shelterinTTAGGG telomeric repeatsTelomeric ssDNACheckpoint responseTelomeric repeatsPOT1Ataxia telangiectasiaCell cycleAberrant accumulationCycling cellsSpecific mannerInteresting modelTelomeresProtein ACritical roleProteinRecent studiesReplicationShelterinRad3KinaseRepeats
2009
Gcn5 and SAGA Regulate Shelterin Protein Turnover and Telomere Maintenance
Atanassov BS, Evrard YA, Multani AS, Zhang Z, Tora L, Devys D, Chang S, Dent SY. Gcn5 and SAGA Regulate Shelterin Protein Turnover and Telomere Maintenance. Molecular Cell 2009, 35: 352-364. PMID: 19683498, PMCID: PMC2749492, DOI: 10.1016/j.molcel.2009.06.015.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedChromosome AberrationsDNA Breaks, Double-StrandedDNA RepairGene DeletionHumansMiceModels, BiologicalP300-CBP Transcription FactorsProteasome InhibitorsProtein StabilityShelterin ComplexTelomereTelomere-Binding ProteinsTelomeric Repeat Binding Protein 1Thiolester HydrolasesUbiquitin ThiolesteraseConceptsSAGA complexTelomeric shelterin complexDeletion of GCN5Accessibility of chromatinBona fide componentTRF1 levelsGene regulationShelterin complexTelomere maintenanceMammalian cellsTranscription factorsGCN5DNA repairFide componentRepair proteinsTelomere dysfunctionProtein turnoverHuman cellsUbiquitin-specific protease 22Biochemical studiesOverexpression of USP22USP22ComplexesTurnoverChromatinMultiple roles for MRE11 at uncapped telomeres
Deng Y, Guo X, Ferguson DO, Chang S. Multiple roles for MRE11 at uncapped telomeres. Nature 2009, 460: 914-918. PMID: 19633651, PMCID: PMC2760383, DOI: 10.1038/nature08196.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAnimalsAtaxia Telangiectasia Mutated ProteinsATP-Binding Cassette TransportersCell Cycle ProteinsCell LineChromosomal Proteins, Non-HistoneChromosome AberrationsDNA DamageDNA Ligase ATPDNA LigasesDNA Repair EnzymesDNA-Binding ProteinsFibroblastsIntracellular Signaling Peptides and ProteinsMiceMRE11 Homologue ProteinNuclear ProteinsShelterin ComplexTelomereTelomere-Binding ProteinsTelomeric Repeat Binding Protein 2Tumor Suppressor p53-Binding Protein 1Tumor Suppressor ProteinsConceptsMRN complexLinear eukaryotic chromosomesDNA double-strand breaksDNA damage repair pathwaysDouble-strand breaksDamage repair pathwaysGenome integrityEukaryotic chromosomesUncapped telomeresTelomere maintenanceRepair factorsDNA endsRepair pathwaysTelomeric endNuclease activityTelomeresMultiple rolesMre11Major playersPathogenic lesionsMre1ChromosomesComplexesProteinAlleles
2007
WRN at telomeres: implications for aging and cancer
Multani AS, Chang S. WRN at telomeres: implications for aging and cancer. Journal Of Cell Science 2007, 120: 713-721. PMID: 17314245, DOI: 10.1242/jcs.03397.Peer-Reviewed Original ResearchConceptsWerner syndromeHuman Werner syndromePremature aging syndromesRecent genetic evidenceAge-related pathologiesGenome stabilityWRN deficiencyTelomere maintenanceDNA replicationGenetic evidenceSingle gene defectsTelomere dysfunctionCellular senescenceAging syndromesMolecular levelPremature agingEarly cancer onsetWRNGene defectsCancer onsetMajor roleTelomeresSenescenceRapid onsetProtein
2006
GCN5 Functions in Telomere Maintenance and Neural Development
Dent S, Evrard Y, Lin W, Bu P, Phan H, Chang S, Multani A. GCN5 Functions in Telomere Maintenance and Neural Development. The FASEB Journal 2006, 20: a1472-a1472. DOI: 10.1096/fasebj.20.5.a1472-e.Peer-Reviewed Original ResearchMutant embryosNeural developmentTelomere maintenanceDouble mutant embryosCatalytic site mutationsNeural tube closureGcn5 functionsTelomere defectsTelomere fusionEmbryonic lethalityHypomorphic alleleProper expressionGCN5Tube closureSite mutationSimilar defectsEnd associationEmbryosFirst evidenceApoptosisBrain developmentP53CellsTelomeresGenes
1999
Longevity, Stress Response, and Cancer in Aging Telomerase-Deficient Mice
Rudolph K, Chang S, Lee H, Blasco M, Gottlieb G, Greider C, DePinho R. Longevity, Stress Response, and Cancer in Aging Telomerase-Deficient Mice. Cell 1999, 96: 701-712. PMID: 10089885, DOI: 10.1016/s0092-8674(00)80580-2.Peer-Reviewed Original ResearchConceptsOrganismal aging processTelomerase-null miceTelomerase-deficient miceTelomere functionOrganismal levelTelomere maintenanceCellular senescenceOverall fitnessPhysiological processesStress responseHematopoietic ablationGenetic instabilityTelomere lengthNull miceCritical roleLife spanWound healingAging processSpontaneous malignanciesSenescenceOrganismsFitnessPathophysiological symptomsRoleMice