2023
Disturbed flow increases endothelial inflammation and permeability via a Frizzled-4-β-catenin-dependent pathway
Rickman M, Ghim M, Pang K, von Huelsen Rocha A, Drudi E, Sureda-Vives M, Ayoub N, Tajadura-Ortega V, George S, Weinberg P, Warboys C. Disturbed flow increases endothelial inflammation and permeability via a Frizzled-4-β-catenin-dependent pathway. Journal Of Cell Science 2023, 136: jcs260449. PMID: 36846872, PMCID: PMC10112981, DOI: 10.1242/jcs.260449.Peer-Reviewed Original ResearchConceptsEndothelial dysfunctionEndothelial cellsFrizzled-4Β-cateninCultured human aortic endothelial cellsR-spondinsHuman aortic endothelial cellsPro-inflammatory genesEndothelial paracellular permeabilityDisturbed flowAortic endothelial cellsCultured endothelial cellsRole of WntEndothelial inflammationAortic archEarly atherogenesisCanonical Wnt pathwayDysfunctionReduced expressionWnt5a signalingParacellular permeabilityWnt pathwayInhibitionKnockdownExpression
2021
Leucine-Rich α-2-Glycoprotein 1 Suppresses Endothelial Cell Activation Through ADAM10-Mediated Shedding of TNF-α Receptor
Pang K, Ghim M, Liu C, Tay H, Fhu C, Chia R, Qiu B, Sarathchandra P, Chester A, Yacoub M, Wilkinson F, Weston R, Warboys C, Hou H, Weinberg P, Wang X. Leucine-Rich α-2-Glycoprotein 1 Suppresses Endothelial Cell Activation Through ADAM10-Mediated Shedding of TNF-α Receptor. Frontiers In Cell And Developmental Biology 2021, 9: 706143. PMID: 34291056, PMCID: PMC8288075, DOI: 10.3389/fcell.2021.706143.Peer-Reviewed Original ResearchTumor necrosis factor receptor 1Critical limb ischemiaSera of patientsEndothelial activationEndothelial cellsLRG1 expressionElevated serum concentrationsPotential therapeutic roleLeucine-Rich αTumor necrosis factorTNF-α receptorWild-type miceEndothelial cell activationNecrosis factor receptor 1Role of LRG1NF-κB activationNF-κB signalingFactor receptor 1STNFR1 concentrationCLI patientsLimb ischemiaSerum concentrationsHealthy controlsInflammatory diseasesTherapeutic role
2018
Investigation of Nanodroplet Adhesion to Endothelial Cells Under Atheroprone Flow Conditions
Zhang G, Pang K, Ghim M, Weinberg P, Tang M. Investigation of Nanodroplet Adhesion to Endothelial Cells Under Atheroprone Flow Conditions. 2018, 00: 1-4. DOI: 10.1109/ultsym.2018.8579749.Peer-Reviewed Original ResearchA novel method for segmenting growth of cells in sheared endothelial culture reveals the secretion of an anti-inflammatory mediator
Ghim M, Pang K, Arshad M, Wang X, Weinberg P. A novel method for segmenting growth of cells in sheared endothelial culture reveals the secretion of an anti-inflammatory mediator. Journal Of Biological Engineering 2018, 12: 15. PMID: 30127849, PMCID: PMC6092814, DOI: 10.1186/s13036-018-0107-6.Peer-Reviewed Original ResearchVascular cell adhesion molecule-1Intercellular adhesion molecule-1Adhesion molecule-1Molecule-1Cell adhesion molecule-1Anti-inflammatory mediatorsAnti-inflammatory effectsPathogenesis of atherosclerosisEndothelial cellsBlood vesselsNormal physiologyEndothelial culturesGrowth of cellsMediatorsConclusionsThe resultsEndothelium