Breeding Based Remobilization of Tol2 Transposon in Xenopus tropicalis
Lane MA, Kimber M, Khokha MK. Breeding Based Remobilization of Tol2 Transposon in Xenopus tropicalis. PLOS ONE 2013, 8: e76807. PMID: 24116167, PMCID: PMC3792888, DOI: 10.1371/journal.pone.0076807.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedBase SequenceBinding SitesBreedingCrosses, GeneticDNA Transposable ElementsEmbryo, NonmammalianFemaleGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsMaleMicroscopy, FluorescenceModels, GeneticMutagenesis, InsertionalReverse Transcriptase Polymerase Chain ReactionTransposasesXenopusConceptsTol2 transposonEnhancer-trapping strategySimple breeding strategiesTransgenic reporter linesX. tropicalisTransgenic linesGene trapBreeding strategiesReporter linesTrap strategyBiological processesTransposonDiverse arrayTransgenesisPowerful modelXenopusGenomeGermlineLinesExpressingRemobilizationTropicalisNew opportunities