Branching in poly(amine-co-ester) polyplexes impacts mRNA transfection
Shin K, Suh H, Suberi A, Whang C, Ene M, Grundler J, Grun M, Saltzman W. Branching in poly(amine-co-ester) polyplexes impacts mRNA transfection. Biomaterials 2024, 311: 122692. PMID: 38986360, PMCID: PMC11298310, DOI: 10.1016/j.biomaterials.2024.122692.Peer-Reviewed Original ResearchPolymer branchingTransfection in vivoPhysicochemical propertiesPoly(amine-co-esterMRNA deliveryTerminal groupsPolymer featuresIn vitroPolymerNucleic acid deliveryMonomer compositionAnalysis of physicochemical propertiesStructural parametersCationic polymersStability of polyplexesMRNA transfectionTransfection efficiencyAcid deliveryDelivery vehiclesTransfectionMRNAModifying the Backbone Chemistry of PEG‐Based Bottlebrush Block Copolymers for the Formation of Long‐Circulating Nanoparticles
Grundler J, Whang C, Shin K, Savan N, Zhong M, Saltzman W. Modifying the Backbone Chemistry of PEG‐Based Bottlebrush Block Copolymers for the Formation of Long‐Circulating Nanoparticles. Advanced Healthcare Materials 2024, 13: e2304040. PMID: 38734871, PMCID: PMC11368614, DOI: 10.1002/adhm.202304040.Peer-Reviewed Original ResearchBottlebrush block copolymersPerformance of nanoparticlesBottlebrush polymersBlock copolymersBiomedical applicationsLinear hydrophilic polymersBackbone chemistryLocation of functional groupsPoly(lactic acidBottlebrush backboneNanoparticle shellHydrophilic polymersPolymer backboneSurface functionalizationHeterobifunctional poly(ethylene glycolPoly(ethylene glycolHierarchical controlBlood circulation half-livesEnhanced tumor accumulationNanoparticlesPolymerReduced cellular uptakeNanoparticle physicochemical propertiesBottlebrushConventional nanoparticlesEnhancing in vivo cell and tissue targeting by modulation of polymer nanoparticles and macrophage decoys
Piotrowski-Daspit A, Bracaglia L, Eaton D, Richfield O, Binns T, Albert C, Gould J, Mortlock R, Egan M, Pober J, Saltzman W. Enhancing in vivo cell and tissue targeting by modulation of polymer nanoparticles and macrophage decoys. Nature Communications 2024, 15: 4247. PMID: 38762483, PMCID: PMC11102454, DOI: 10.1038/s41467-024-48442-7.Peer-Reviewed Original ResearchConceptsPoly(amine-co-esterPolymer nanoparticlesDelivery of nucleic acid therapeuticsCell-type tropismTissue tropismNucleic acid delivery vehiclesIn vivo deliveryIn vivo efficacyCirculation half-lifeNucleic acid therapeuticsVehicle characteristicsTunable propertiesBiodistribution assessmentPhysiological fatePolymer chemistrySurface propertiesPharmacokinetic modelTissue targetingNanoparticlesDistribution modifiersPolymeric nanoparticlesTropismPolymerDelivery vehiclesHalf-life