2007
Myosin 1E interacts with synaptojanin‐1 and dynamin and is involved in endocytosis
Krendel M, Osterweil EK, Mooseker MS. Myosin 1E interacts with synaptojanin‐1 and dynamin and is involved in endocytosis. FEBS Letters 2007, 581: 644-650. PMID: 17257598, PMCID: PMC1861834, DOI: 10.1016/j.febslet.2007.01.021.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsChlorocebus aethiopsClathrin-Coated VesiclesCOS CellsDynaminsEndocytosisHeLa CellsHumansImmunoprecipitationMiceMyosin Type INerve Tissue ProteinsPhosphoric Monoester HydrolasesProtein BindingProtein TransportRatsSrc Homology DomainsSynapsesTissue ExtractsTransferrinTwo-Hybrid System TechniquesConceptsSH3 domainMyosin 1eSynaptojanin 1Myosin IIntact SH3 domainDominant-negative mannerReceptor-mediated endocytosisHigher eukaryotesArp2/3 complexInhibits endocytosisPlasma membraneActin polymerizationImportant regulatorEndocytosisHuman class IProminent functionDynaminTail regionDomainEukaryotesClathrinYeastRegulatorProteinBinds
2004
A role for myosin-1A in the localization of a brush border disaccharidase
Tyska M, Mooseker M. A role for myosin-1A in the localization of a brush border disaccharidase. Journal Of Cell Biology 2004, 165: 395-405. PMID: 15138292, PMCID: PMC2172191, DOI: 10.1083/jcb.200310031.Peer-Reviewed Original Research
2002
MYO1A (Brush Border Myosin I) Dynamics in the Brush Border of LLC-PK1-CL4 Cells
Tyska M, Mooseker M. MYO1A (Brush Border Myosin I) Dynamics in the Brush Border of LLC-PK1-CL4 Cells. Biophysical Journal 2002, 82: 1869-1883. PMID: 11916846, PMCID: PMC1301984, DOI: 10.1016/s0006-3495(02)75537-9.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBiophysical PhenomenaBiophysicsCloning, MolecularCytoskeletonElectrophoresis, Polyacrylamide GelGreen Fluorescent ProteinsHumansKidneyKineticsLLC-PK1 CellsLuminescent ProteinsMicroscopy, ConfocalMicroscopy, FluorescenceMicrovilliMyosin Type IPrecipitin TestsProtein Structure, TertiaryRecombinant Fusion ProteinsSubcellular FractionsSwineTime FactorsTransfectionConceptsTail domainLLC-PK1Actin core bundleKidney epithelial cell lineApical targetingActin dynamicsBrush borderActin turnoverGFP-actinBB populationsEpithelial cell lineActin domainsFluorescence recoveryCl4 cellsRapid turnoverApical surfaceMotor domainCore bundleATP depletionCell linesActinTurnoverCellsDomain
2001
Myosin-I nomenclature
Gillespie P, Albanesi J, Bähler M, Bement W, Berg J, Burgess D, Burnside B, Cheney R, Corey D, Coudrier E, de Lanerolle P, Hammer J, Hasson T, Holt J, Hudspeth AJ, Ikebe M, Kendrick-Jones J, Korn E, Li R, Mercer J, Milligan R, Mooseker M, Ostap E, Petit C, Pollard T, Sellers J, Soldati T, Titus M. Myosin-I nomenclature. Journal Of Cell Biology 2001, 155: 703-704. PMID: 11724811, PMCID: PMC2150864, DOI: 10.1083/jcb.200110032.Peer-Reviewed Original Research
2000
Class V myosins
Reck-Peterson S, Provance D, Mooseker M, Mercer J. Class V myosins. Biochimica Et Biophysica Acta 2000, 1496: 36-51. PMID: 10722875, DOI: 10.1016/s0167-4889(00)00007-0.Peer-Reviewed Original Research
1999
An invasion-associated Salmonella protein modulates the actin-bundling activity of plastin
Zhou D, Mooseker M, Galán J. An invasion-associated Salmonella protein modulates the actin-bundling activity of plastin. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 10176-10181. PMID: 10468582, PMCID: PMC17862, DOI: 10.1073/pnas.96.18.10176.Peer-Reviewed Original ResearchConceptsActin-bundling activityT-plastinBacterial-host cell contactHost cell signal transduction pathwaysHost cellsType III secretion systemBacterial effector proteinsActin-binding proteinsSignal transduction pathwaysBacterial effectorsMembrane rufflesEffector proteinsActin cytoskeletonMembrane rufflingSecretion systemSalmonella proteinsBacterial entryBacterial internalizationSalmonella entrySignaling processesActin filamentsF-actinNonphagocytic cellsProteinCell contactRole of the S. typhimurium Actin-Binding Protein SipA in Bacterial Internalization
Zhou D, Mooseker M, Galán J. Role of the S. typhimurium Actin-Binding Protein SipA in Bacterial Internalization. Science 1999, 283: 2092-2095. PMID: 10092234, DOI: 10.1126/science.283.5410.2092.Peer-Reviewed Original ResearchCloning and characterization of mouse brush border myosin‐I in adult and embryonic intestine
Skowron J, Mooseker M. Cloning and characterization of mouse brush border myosin‐I in adult and embryonic intestine. Journal Of Experimental Zoology 1999, 283: 242-257. PMID: 9933937, DOI: 10.1002/(sici)1097-010x(19990215)283:3<242::aid-jez3>3.0.co;2-f.Peer-Reviewed Original ResearchConceptsBrush border myosinMicrovillar actin bundlesEmbryonic intestineSubcellular localization profileBasolateral membrane domainsGenomic clonesCalmodulin light chainsVertebrate speciesMembrane domainsActin corePlasma membraneKnockout strategiesActin bundlesImmunolocalization studiesIntestinal epithelial cellsGolgi apparatusNorthern analysisPrimary structureNorthern blotSitu localizationLocalization profilesRNA expressionEmbryosDirect targetingEmbryogenesis
1998
Human brush border myosin‐I and myosin‐Ic expression in human intestine and Caco‐2BBe cells
Skowron J, Bement W, Mooseker M. Human brush border myosin‐I and myosin‐Ic expression in human intestine and Caco‐2BBe cells. Cytoskeleton 1998, 41: 308-324. PMID: 9858156, DOI: 10.1002/(sici)1097-0169(1998)41:4<308::aid-cm4>3.0.co;2-j.Peer-Reviewed Original ResearchHuman myosin-IXb is a mechanochemically active motor and a GAP for rho
Post P, Bokoch G, Mooseker M. Human myosin-IXb is a mechanochemically active motor and a GAP for rho. Journal Of Cell Science 1998, 111: 941-950. PMID: 9490638, DOI: 10.1242/jcs.111.7.941.Peer-Reviewed Original ResearchActinsAdenosine TriphosphateAlternative SplicingAmino Acid SequenceAnimalsCalciumCalmodulinGTP PhosphohydrolasesGTPase-Activating ProteinsGTP-Binding ProteinsHumansLeukocytesMolecular Sequence DataMuscle ContractionMyosinsProtein BindingProteinsRas GTPase-Activating ProteinsRas ProteinsRatsRho GTP-Binding ProteinsUnconventional Myosins in Cell Movement, Membrane Traffic, and Signal Transduction
Mermall V, Post P, Mooseker M. Unconventional Myosins in Cell Movement, Membrane Traffic, and Signal Transduction. Science 1998, 279: 527-533. PMID: 9438839, DOI: 10.1126/science.279.5350.527.Peer-Reviewed Original ResearchConceptsSignal transductionCell movementMembrane trafficMembrane traffickingDisease-causing mutationsCellular functionsMyosin genesImportance of myosinUnconventional myosinMyosin functionCellular levelMolecular motorsMyosin structureTransductionMyosinGenesTraffickingActinMutationsBiochemicalFunctionMembersTargetIdentification
1997
The growing family of myosin motors and their role in neurons and sensory cells
Hasson T, Mooseker M. The growing family of myosin motors and their role in neurons and sensory cells. Current Opinion In Neurobiology 1997, 7: 615-623. PMID: 9384540, DOI: 10.1016/s0959-4388(97)80080-3.Peer-Reviewed Original ResearchUnconventional Myosins in Inner-Ear Sensory Epithelia
Hasson T, Gillespie P, Garcia J, MacDonald R, Zhao Y, Yee A, Mooseker M, Corey D. Unconventional Myosins in Inner-Ear Sensory Epithelia. Journal Of Cell Biology 1997, 137: 1287-1307. PMID: 9182663, PMCID: PMC2132524, DOI: 10.1083/jcb.137.6.1287.Peer-Reviewed Original ResearchThe Genomic Structure of the Gene Defective in Usher Syndrome Type Ib (MYO7A)
Kelley P, Weston M, Chen Z, Orten D, Hasson T, Overbeck L, Pinnt J, Talmadge C, Ing P, Mooseker M, Corey D, Sumegi J, Kimberling W. The Genomic Structure of the Gene Defective in Usher Syndrome Type Ib (MYO7A). Genomics 1997, 40: 73-79. PMID: 9070921, DOI: 10.1006/geno.1996.4545.Peer-Reviewed Original ResearchEffects of shaker‐1 mutations on myosin‐VIIa protein and mRNA expression
Hasson T, Walsh J, Cable J, Mooseker M, Brown S, Steel K. Effects of shaker‐1 mutations on myosin‐VIIa protein and mRNA expression. Cytoskeleton 1997, 37: 127-138. PMID: 9186010, DOI: 10.1002/(sici)1097-0169(1997)37:2<127::aid-cm5>3.0.co;2-5.Peer-Reviewed Original ResearchConceptsShaker-1 mutationsWild-type levelsNorthern blot analysisMammalian diseasesActin cytoskeletonMyosin VIIaShaker-1 miceGene expressionUnconventional myosinMRNA expressionProteinMyosin-VIIa mutationsImmunoblot analysisMotor domainRange of expressionMutationsBlot analysisAllelesProtein expressionTissue functionExpressionLife spanSH1Inner earLight microscopic levelActin-binding membrane proteins identified by F-actin blot overlays.
Luna E, Pestonjamasp K, Cheney R, Strassel C, Lu T, Chia C, Hitt A, Fechheimer M, Furthmayr H, Mooseker M. Actin-binding membrane proteins identified by F-actin blot overlays. Society Of General Physiologists Series 1997, 52: 3-18. PMID: 9210216.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsActinsAmino Acid SequenceAnimalsBlotting, WesternBrainBreast NeoplasmsCattleChick EmbryoDictyosteliumElectrophoresis, Polyacrylamide GelHeLa CellsHumansIodine RadioisotopesMammalsMembrane ProteinsMiceMicrofilament ProteinsNeuroblastomaNeuropeptidesNeutrophilsSodium Dodecyl SulfateTumor Cells, CulturedConceptsBlot overlayApparent molecular massMembrane proteinsF-actinF-actin binding proteinMolecular massControl cell shapePeripheral membrane proteinsSpecialized membrane domainsCell-cell adhesionPlasma membrane-enriched fractionActin-binding proteinsMammalian cell linesCell surface extensionsMembrane-enriched fractionMembrane rufflesProtein 4.1Membrane domainsMammalian cellsDictyostelium discoideumSoil amoebaPseudopod dynamicsCell shapeEfficient chemotaxisMembrane bilayer
1996
Myosin VIIA mutation screening in 189 Usher syndrome type 1 patients.
Weston M, Kelley P, Overbeck L, Wagenaar M, Orten D, Hasson T, Chen Z, Corey D, Mooseker M, Sumegi J, Cremers C, Moller C, Jacobson S, Gorin M, Kimberling W. Myosin VIIA mutation screening in 189 Usher syndrome type 1 patients. American Journal Of Human Genetics 1996, 59: 1074-83. PMID: 8900236, PMCID: PMC1914835.Peer-Reviewed Original ResearchConceptsCongenital profound hearing lossMyosin-VIIa mutationsType 1 patientsProfound hearing lossUsher syndrome type 1BUnique mutationsPresence of mutationsAutosomal recessive disorderVestibular abnormalitiesMyosin VIIA geneHearing lossRetinitis pigmentosaPatientsType 1bI casesRecessive disorderSingle caseCompound heterozygotesPremature stop codonSimple heterozygotesGreater percentageUnrelated control samplesDisordersMutant allelesCurrent studyMapping of Unconventional Myosins in Mouse and Human
Hasson T, Skowron J, Gilbert D, Avraham K, Perry W, Bement W, Anderson B, Sherr E, Chen Z, Greene L, Ward D, Corey D, Mooseker M, Copeland N, Jenkins N. Mapping of Unconventional Myosins in Mouse and Human. Genomics 1996, 36: 431-439. PMID: 8884266, DOI: 10.1006/geno.1996.0488.Peer-Reviewed Original ResearchMolecular Cloning and Domain Structure of Human Myosin-VIIa, the Gene Product Defective in Usher Syndrome 1B
Chen Z, Hasson T, Kelley P, Schwender B, Schwartz M, Ramakrishnan M, Kimberling W, Mooseker M, Corey D. Molecular Cloning and Domain Structure of Human Myosin-VIIa, the Gene Product Defective in Usher Syndrome 1B. Genomics 1996, 36: 440-448. PMID: 8884267, DOI: 10.1006/geno.1996.0489.Peer-Reviewed Original ResearchConceptsUsher syndrome 1BIntestinal epithelium cell lineProgressive retinal degenerationEpithelium cell lineVestibular functionMyosin VIIaPigment epitheliumRetinal degenerationInner earReceptor cellsRT-PCRBand 4.1 familyRestricted expressionShort formCell linesMembrane-associated portionHuman myosin VIIaMolecular cloningLong transcriptsTestisShort transcriptsUnconventional myosinLong repeatsHigh abundanceAmino acidsVertebrate Unconventional Myosins*
Hasson T, Mooseker M. Vertebrate Unconventional Myosins*. Journal Of Biological Chemistry 1996, 271: 16431-16434. PMID: 8690736, DOI: 10.1074/jbc.271.28.16431.Peer-Reviewed Original Research