2024
Regulatory CD4+ T cells redirected against pathogenic CD8+ T cells protect NOD mice from development of autoimmune diabetes
Kakabadse D, Chen D, Fishman S, Weinstein-Marom H, Davies J, Wen L, Gross G, Wong F. Regulatory CD4+ T cells redirected against pathogenic CD8+ T cells protect NOD mice from development of autoimmune diabetes. Frontiers In Immunology 2024, 15: 1463971. PMCID: PMC11439686, DOI: 10.3389/fimmu.2024.1463971.Peer-Reviewed Original ResearchCD8+ T cellsCD4+ T cellsAntigen-specific CD8+ T cellsDevelopment of autoimmune diabetesRegulatory T cellsCo-transfer experimentsT cellsNOD miceAutoimmune diabetesAntigen-specific CD4+ T cellsRegulatory CD4+ T cellsAntigen-specific cytotoxic CD8Pathogenic CD8+ T cellsPre-diabetic NOD micePolyclonal CD4+ T cellsDevelopment of type 1 diabetesSuppresses autoimmune diabetesAntigen-specific CD4Expression of Foxp3Young NOD miceT cell-T cellMarkers in vitroType 1 diabetesAdoptive transferTreg cellsBile acid-gut microbiota imbalance in cholestasis and its long-term effect in mice
Yang X, Xu Y, Li J, Ran X, Gu Z, Song L, Zhang L, Wen L, Ji G, Wang R. Bile acid-gut microbiota imbalance in cholestasis and its long-term effect in mice. MSystems 2024, 9: e00127-24. PMID: 38934542, PMCID: PMC11265269, DOI: 10.1128/msystems.00127-24.Peer-Reviewed Original ResearchGut microbiotaGut microbiota dysbiosisDysregulated gut microbiotaGut microbiota transplantationGerm-free miceVirulence factorsSmall intestinal bacteriaBile acid homeostasisMicrobiota dysbiosisDecreased diversityMicrobiota imbalanceMicrobiotaMouse model of cholestasisBile acidsMouse modelIntestinal bacteriaAcid homeostasisMicrobiota transplantationClinical management of patientsGutHepatic bile acidsLong-term effectsPre-clinical findingsModel of cholestasisManagement of patientsGut microbiota from B-cell-specific TLR9-deficient NOD mice promote IL-10+ Breg cells and protect against T1D
Yang X, Huang J, Peng J, Wang P, Wong F, Wang R, Wang D, Wen L. Gut microbiota from B-cell-specific TLR9-deficient NOD mice promote IL-10+ Breg cells and protect against T1D. Frontiers In Immunology 2024, 15: 1413177. PMID: 38903498, PMCID: PMC11187306, DOI: 10.3389/fimmu.2024.1413177.Peer-Reviewed Original ResearchGut microbiotaGerm-free miceToll-like receptor 9Increased gut permeabilityIntestinal microbiotaGut permeabilityT1D developmentGut microbiota compositionFecal samplesTransferred to germ-free miceGut barrier integrityBreg cell differentiationMicrobiota influenceMucin degradationMicrobiota compositionBreg cellsAltered microbiotaMicrobiota impactMicrobiotaGene expressionImmune regulationDevelopment of T1DCell differentiationGutNOD miceTlr9 deficiency in B cells leads to obesity by promoting inflammation and gut dysbiosis
Wang P, Yang X, Zhang L, Sha S, Huang J, Peng J, Gu J, Pearson J, Hu Y, Zhao H, Wong F, Wang Q, Wen L. Tlr9 deficiency in B cells leads to obesity by promoting inflammation and gut dysbiosis. Nature Communications 2024, 15: 4232. PMID: 38762479, PMCID: PMC11102548, DOI: 10.1038/s41467-024-48611-8.Peer-Reviewed Original ResearchConceptsToll-like receptor 9Gut microbiotaGut microbial communityTransferred to germ-free miceB cellsGerm-free miceTLR9 deficiencyKO miceGene sequencesGerminal center B cellsMicrobial communitiesMarginal zone B cellsGut dysbiosisFollicular helper cellsSelf-DNAMetabolic homeostasisAssociated with increased frequencyPro-inflammatory stateFat tissue inflammationGutHigh-fat dietMicrobiotaHelper cellsT cellsControl miceMulti-purposed diagnostic system for ovarian endometrioma using CNN and transformer networks in ultrasound
Li Y, Zhao B, Wen L, Huang R, Ni D. Multi-purposed diagnostic system for ovarian endometrioma using CNN and transformer networks in ultrasound. Biomedical Signal Processing And Control 2024, 91: 105923. DOI: 10.1016/j.bspc.2023.105923.Peer-Reviewed Original ResearchFirst deep learning methodDeep learning methodsAccuracy of classificationDeep learningSegmentation branchTransformer networkClassification branchPopular networksAttention mechanismDice scoreCyst segmentationLearning methodsClassification accuracyComparable performanceExperience of doctorsReader studyDiagnostic systemNetworkClassificationSpecific informationAccuracyImagesCNNCyst regionExtensive datasetGut microbiota and therapy for obesity and type 2 diabetes
Zhang L, Wang P, Huang J, Xing Y, Wong F, Suo J, Wen L. Gut microbiota and therapy for obesity and type 2 diabetes. Frontiers In Endocrinology 2024, 15: 1333778. PMID: 38596222, PMCID: PMC11002083, DOI: 10.3389/fendo.2024.1333778.Peer-Reviewed Original ResearchGut microbiota compositionGut microbiotaMicrobiota compositionType 2 diabetesFunction of gut microbiotaHomeostasis of gut microbiotaMicrobiota-based approachesModulating gut microbiotaBariatric surgeryEffective anti-obesity treatmentQuality of life of individualsMicrobiotaMildly obese patientsSustained weight lossAssociated with obesityDiminished quality of lifeAnti-obesity treatmentObesity management strategiesGutQuality of lifeNon-surgical interventionsIncreased medical costsPublic health crisisMusculoskeletal disordersObese patientsTLR5-deficiency controls dendritic cell subset development in an autoimmune diabetes-susceptible model
Pearson J, Hu Y, Peng J, Wong F, Wen L. TLR5-deficiency controls dendritic cell subset development in an autoimmune diabetes-susceptible model. Frontiers In Immunology 2024, 15: 1333967. PMID: 38482010, PMCID: PMC10935730, DOI: 10.3389/fimmu.2024.1333967.Peer-Reviewed Original ResearchConceptsToll-like receptor 5Antigen-presenting cellsDendritic cellsType 1 diabetesTLR5-deficientDC developmentCytokine secretionCD4<sup>+</sup> T cell proliferationPathogenesis of type 1 diabetesT cell responsesEnhanced cytokine secretionT cell proliferationWild-type miceSusceptibility to obesitySusceptibility to T1DProinflammatory cytokine secretionGut microbiotaSpontaneous T1DNOD miceAutoimmune diabetesNon-obeseHuman T1DReceptor 5Autoimmune diseasesHyper-secretion
2023
Comparison of the diagnostic efficiency between the O-RADS US risk stratification system and doctors’ subjective judgment
Zhou S, Guo Y, Wen L, Liu J, Fu Y, Xu F, Liu M, Zhao B. Comparison of the diagnostic efficiency between the O-RADS US risk stratification system and doctors’ subjective judgment. BMC Medical Imaging 2023, 23: 190. PMID: 37986051, PMCID: PMC10662783, DOI: 10.1186/s12880-023-01153-9.Peer-Reviewed Original ResearchNovel engineered B lymphocytes targeting islet-specific T cells inhibit the development of type 1 diabetes in non-obese diabetic Scid mice
Chen D, Kakabadse D, Fishman S, Weinstein-Marom H, Davies J, Boldison J, Thayer T, Wen L, Gross G, Wong F. Novel engineered B lymphocytes targeting islet-specific T cells inhibit the development of type 1 diabetes in non-obese diabetic Scid mice. Frontiers In Immunology 2023, 14: 1227133. PMID: 37731505, PMCID: PMC10507356, DOI: 10.3389/fimmu.2023.1227133.Peer-Reviewed Original ResearchConceptsAntigen-specific CD8Islet-specific T cellsT cellsAutoimmune diabetesB cellsSCID miceMouse modelB lymphocytesNon-obese diabetic (NOD) mouse modelRegulatory B cell functionsProtective cell typesAntigen-specific CD4Pathogenic T cellsT cell cytotoxicityAntigen-presenting cellsCo-transfer experimentsDiabetic mouse modelDiabetic SCID miceType 1 diabetesAntigen-specific cellsB cell functionNovel therapeutic approachesMHC II moleculesSplenic B cellsPD-1Distinct signatures of gut microbiota and metabolites in different types of diabetes: a population-based cross-sectional study
Hu J, Ding J, Li X, Li J, Zheng T, Xie L, Li C, Tang Y, Guo K, Huang J, Liu S, Yan J, Peng W, Hou C, Wen L, Xu A, Zhou Z, Xiao Y. Distinct signatures of gut microbiota and metabolites in different types of diabetes: a population-based cross-sectional study. EClinicalMedicine 2023, 62: 102132. PMID: 37593224, PMCID: PMC10430172, DOI: 10.1016/j.eclinm.2023.102132.Peer-Reviewed Original ResearchAdult-onset T1DHealthy controlsGut microbiotaT1D patientsShort-chain fatty acid-producing bacteriaPopulation-based cross-sectional studyGut microbial alterationsRecent epidemiological dataType 1 diabetesCross-sectional studyT2D patientsIntestinal disturbancesDifferential diagnosisSerum metabolitesEpidemiological dataPatientsStudy subjectsT1DDiabetesSignificant differencesAcid-producing bacteriaMicrobiotaMicrobial profilesNational Key ResearchMicrobial alterationsAssessing Obstetric Anal Sphincter Injuries
Zhao B, Li Y, Tang Y, Guo Y, Yang Y, Wen L, Dietz H. Assessing Obstetric Anal Sphincter Injuries. Journal Of Ultrasound In Medicine 2023, 42: 2031-2038. PMID: 36916688, DOI: 10.1002/jum.16221.Peer-Reviewed Original ResearchNLRP6 deficiency expands a novel CD103+ B cell population that confers immune tolerance in NOD mice
Pearson J, Peng J, Huang J, Yu X, Tai N, Hu Y, Sha S, Flavell R, Zhao H, Wong F, Wen L. NLRP6 deficiency expands a novel CD103+ B cell population that confers immune tolerance in NOD mice. Frontiers In Immunology 2023, 14: 1147925. PMID: 36911699, PMCID: PMC9995752, DOI: 10.3389/fimmu.2023.1147925.Peer-Reviewed Original ResearchConceptsNlrp6-deficient miceType 1 diabetesNLRP6 deficiencyB cellsIL-10Non-obese diabetic (NOD) miceType 1 diabetes developmentRole of NLRP6Germ-free miceT cell proliferationB cell populationsIntestinal epithelial cellsBreg populationAutoimmune diabetesNOD miceCrohn's diseaseImmune toleranceDiabetes developmentDiabetic miceImmune cellsCD103Inflammasome proteinsImmune responseNLRP6Gut microbiotaDifferences in clinical features and gut microbiota between individuals with methamphetamine casual use and methamphetamine use disorder
He L, Yang B, Ma Y, Wen L, Liu F, Zhang X, Liu T. Differences in clinical features and gut microbiota between individuals with methamphetamine casual use and methamphetamine use disorder. Frontiers In Cellular And Infection Microbiology 2023, 13: 1103919. PMID: 36909722, PMCID: PMC9996337, DOI: 10.3389/fcimb.2023.1103919.Peer-Reviewed Original ResearchConceptsMA use disorderClinical featuresGut microbiotaWithdrawal symptomsMA usersEnterotype 2Use disordersGM diversityBody mass indexMethamphetamine use disorderSemi-Structured AssessmentMass indexNeuropsychiatric symptomsHigh incidenceDiagnostic criteriaDifferential microbesDistinct enterotypesDrug dependenceGM differencesSymptomsClinical traitsPerformed network analysisStrong cravingsFecal samplesFurther studies
2022
Correction: Toll-like receptor 9 deficiency induces osteoclastic bone loss via gut microbiota-associated systemic chronic inflammation
Ding P, Tan Q, Wei Z, Chen Q, Wang C, Qi L, Wen L, Zhang C, Yao C. Correction: Toll-like receptor 9 deficiency induces osteoclastic bone loss via gut microbiota-associated systemic chronic inflammation. Bone Research 2022, 10: 47. PMID: 35798700, PMCID: PMC9263109, DOI: 10.1038/s41413-022-00221-0.Peer-Reviewed Original ResearchCarbonyl Posttranslational Modification Associated With Early-Onset Type 1 Diabetes Autoimmunity.
Yang ML, Connolly SE, Gee RJ, Lam TT, Kanyo J, Peng J, Guyer P, Syed F, Tse HM, Clarke SG, Clarke CF, James EA, Speake C, Evans-Molina C, Arvan P, Herold KC, Wen L, Mamula MJ. Carbonyl Posttranslational Modification Associated With Early-Onset Type 1 Diabetes Autoimmunity. Diabetes 2022, 71: 1979-1993. PMID: 35730902, PMCID: PMC9450849, DOI: 10.2337/db21-0989.Peer-Reviewed Original ResearchConceptsType 1 diabetesNOD miceMurine type 1 diabetesHuman type 1 diabetesDecreased glucose-stimulated insulin secretionAnti-insulin autoimmunityPrediabetic NOD miceGlucose-stimulated insulin secretionOnset Type 1T cell responsesOnset of hyperglycemiaCirculation of patientsAutoreactive CD4Insulin ratioInsulin secretionDiabetesPancreatic isletsType 1Islet proteinsOxidative stressAutoimmunitySelect groupMiceCarbonyl modificationOnsetToll-like receptor 9 deficiency induces osteoclastic bone loss via gut microbiota-associated systemic chronic inflammation
Ding P, Tan Q, Wei Z, Chen Q, Wang C, Qi L, Wen L, Zhang C, Yao C. Toll-like receptor 9 deficiency induces osteoclastic bone loss via gut microbiota-associated systemic chronic inflammation. Bone Research 2022, 10: 42. PMID: 35624094, PMCID: PMC9142495, DOI: 10.1038/s41413-022-00210-3.Peer-Reviewed Original ResearchToll-like receptorsSystemic chronic inflammationBone lossGut microbiotaSystemic inflammationChronic inflammationBone metabolismLow-grade systemic chronic inflammationActivation of TLRsInflammation-induced osteoclastogenesisOsteoclastic bone lossExpansion of CD4Low bone massSubsequent bone lossInflammatory cytokinesBone massT cellsInflammationOsteoclast differentiationBone marrowMyeloid-biased hematopoiesisImmune systemHematopoietic stem cellsSingle-cell RNA sequencingMiceIgM-associated gut bacteria in obesity and type 2 diabetes in C57BL/6 mice and humans
Pearson JA, Ding H, Hu C, Peng J, Galuppo B, Wong FS, Caprio S, Santoro N, Wen L. IgM-associated gut bacteria in obesity and type 2 diabetes in C57BL/6 mice and humans. Diabetologia 2022, 65: 1398-1411. PMID: 35587276, PMCID: PMC9283171, DOI: 10.1007/s00125-022-05711-8.Peer-Reviewed Original ResearchConceptsFecal microbiota transplantType 2 diabetesNormal glucose toleranceB6 miceWild-type miceGlucose toleranceIgM antibodiesObese youthGut microbiotaWeight gainGut bacteriaObese young individualsImpaired glucose toleranceDiet-induced obesityConclusions/interpretationOur resultsBody weight gainGreater weight gainMice fecal microbiotaHuman stool samplesGlucose intoleranceClinical featuresC57BL/6 miceMicrobiota transplantRecipient miceStool samplesObesity aggravates contact hypersensitivity reaction in mice
Majewska‐Szczepanik M, Kowalczyk P, Marcińska K, Strzępa A, Lis GJ, Wong FS, Szczepanik M, Wen L. Obesity aggravates contact hypersensitivity reaction in mice. Contact Dermatitis 2022, 87: 28-39. PMID: 35234303, PMCID: PMC9949724, DOI: 10.1111/cod.14088.Peer-Reviewed Original ResearchConceptsContact hypersensitivityFecal microbiota transplantationQuantitative polymerase chain reactionIL-17AObese miceEnhanced contact hypersensitivityGut microbiota dysbiosisLow-grade inflammationContact hypersensitivity reactionInfluence of obesityInflammatory skin diseaseT helper 1Antigen-specific responsesHigh-fat dietSubcutaneous adipose tissueProinflammatory CD4Proinflammatory milieuCytokine profileMicrobiota dysbiosisDendritic cellsLymph nodesMicrobiota transplantationHelper 1Hypersensitivity reactionsImmune cellsEditorial: Immunopathology of Type 1 Diabetes
Green EA, Cooke AC, Piganelli JD, Richardson SJ, Wen L, Wong FS. Editorial: Immunopathology of Type 1 Diabetes. Frontiers In Immunology 2022, 13: 852963. PMID: 35359940, PMCID: PMC8960371, DOI: 10.3389/fimmu.2022.852963.Peer-Reviewed Original Research
2021
Circadian Rhythm Modulation of Microbes During Health and Infection
Pearson JA, Voisey AC, Boest-Bjerg K, Wong FS, Wen L. Circadian Rhythm Modulation of Microbes During Health and Infection. Frontiers In Microbiology 2021, 12: 721004. PMID: 34512600, PMCID: PMC8430216, DOI: 10.3389/fmicb.2021.721004.Peer-Reviewed Original ResearchGut bacteriaProtective immune responseBeneficial therapeutic responseCircadian rhythm modulationVaccination responseTherapeutic responseTherapeutic administrationImmune responseTherapeutic successCircadian rhythm effectsHost immunityRhythm modulationAdverse effectsInfectionDay-dependent changesAdministration strategiesAltered susceptibilityRhythm effectsCircadian rhythmHealthCircadian modulationVirusOptimal timePhysiological processesResponse