2019
Comparative 3D genome organization in apicomplexan parasites
Bunnik EM, Venkat A, Shao J, McGovern KE, Batugedara G, Worth D, Prudhomme J, Lapp SA, Andolina C, Ross LS, Lawres L, Brady D, Sinnis P, Nosten F, Fidock DA, Wilson EH, Tewari R, Galinski MR, Ben Mamoun C, Ay F, Le Roch KG. Comparative 3D genome organization in apicomplexan parasites. Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 3183-3192. PMID: 30723152, PMCID: PMC6386730, DOI: 10.1073/pnas.1810815116.Peer-Reviewed Original ResearchConceptsGenome organizationGene expressionApicomplexan parasitesVirulence genesSpatial genome organizationPositioning of chromosomesRelated apicomplexan parasitesVirulence gene clusterClustering of centromeresHi-C experimentsStrong repressive effectHuman malaria parasiteChromosome foldingEukaryotic cellsGene familyGene clusterGenome modelGenomeGene clusteringMore virulent pathogensNuclear spaceRepressive effectGenesAntigenic variationVirulent pathogens
2018
Insights into the evolution and drug susceptibility of Babesia duncani from the sequence of its mitochondrial and apicoplast genomes
Virji AZ, Thekkiniath J, Ma W, Lawres L, Knight J, Swei A, Roch KL, Mamoun C. Insights into the evolution and drug susceptibility of Babesia duncani from the sequence of its mitochondrial and apicoplast genomes. International Journal For Parasitology 2018, 49: 105-113. PMID: 30176236, PMCID: PMC6395566, DOI: 10.1016/j.ijpara.2018.05.008.Peer-Reviewed Original ResearchConceptsMitochondrial genomeApicoplast genomeB. duncaniCytochrome c oxidaseB. microtiOrganelle proteinsCircular moleculeApicomplexan parasitesPhylogenetic analysisNew lineageGene transcriptionCytochrome bGenomeC oxidaseI proteinPlasmodium sppProteinTheileria sppBabesia bovisKbParasite factorsCausative agentParasitesAnnotationSpp
2016
Genome-wide diversity and gene expression profiling of Babesia microti isolates identify polymorphic genes that mediate host-pathogen interactions
Silva JC, Cornillot E, McCracken C, Usmani-Brown S, Dwivedi A, Ifeonu OO, Crabtree J, Gotia HT, Virji AZ, Reynes C, Colinge J, Kumar V, Lawres L, Pazzi JE, Pablo JV, Hung C, Brancato J, Kumari P, Orvis J, Tretina K, Chibucos M, Ott S, Sadzewicz L, Sengamalay N, Shetty AC, Su Q, Tallon L, Fraser CM, Frutos R, Molina DM, Krause PJ, Ben Mamoun C. Genome-wide diversity and gene expression profiling of Babesia microti isolates identify polymorphic genes that mediate host-pathogen interactions. Scientific Reports 2016, 6: 35284. PMID: 27752055, PMCID: PMC5082761, DOI: 10.1038/srep35284.Peer-Reviewed Original ResearchConceptsGene expression profilingExpression profilingGenome-wide diversityHost-parasite interactionsHost-pathogen interactionsParasite secretomeParasite evolutionIntraerythrocytic protozoan parasiteGenome compositionGenetic diversityImmuno-proteomic approachSurface proteomeGenetic variationMammalian hostsSmall mammalsSelective pressurePolymorphic genesGenesFirst insightProtozoan parasiteBabesia microtiSecretomeProteome arrayStrong host immune responseHost immune responseA targeted immunomic approach identifies diagnostic antigens in the human pathogen Babesia microti
Cornillot E, Dassouli A, Pachikara N, Lawres L, Renard I, Francois C, Randazzo S, Brès V, Garg A, Brancato J, Pazzi JE, Pablo J, Hung C, Teng A, Shandling AD, Huynh VT, Krause PJ, Lepore T, Delbecq S, Hermanson G, Liang X, Williams S, Molina DM, Ben Mamoun C. A targeted immunomic approach identifies diagnostic antigens in the human pathogen Babesia microti. Transfusion 2016, 56: 2085-2099. PMID: 27184823, PMCID: PMC5644385, DOI: 10.1111/trf.13640.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, ProtozoanBabesia microtiBabesiosisBiomarkersGenome, ProtozoanHumansKineticsMiceProtein Array AnalysisConceptsB. microti antibodiesTransfusion-transmitted babesiosisMicroti antibodiesB. microti-infected miceImmunomics approachBabesia microtiB. microti infectionPromising candidate biomarkerBlood screeningMicroti infectionPast infectionBlood donorsHealth burdenBlood supplyImmune responseAntibody assaysAcute babesiosisEndemic areasHuman studiesBabesiosis infectionDrug AdministrationCandidate biomarkersGood biomarkerStudy designInfection